Abstract: The present disclosure relates to microfluidic devices adapted for post-centrifugation use with selective sample extraction, and methods for their use. Certain embodiments make use of a dye-selective sample extraction. Other embodiments make use of a geographically-selective sample extraction. Other embodiments are also disclosed.

Abstract: A high speed fluid switch is used to separate desired particles from a source stream containing particles, such as cells. A droplet generator creates and regulates a stream of droplets, which intersects with a source stream containing the particles to be sorted. The stream of droplets can be regulated such that portions of the source stream containing desired particles are deflected into a collection vessel, or such that portions of the source stream containing desired particles are permitted to pass uninterrupted. The high speed fluid switch may be implemented on a microfluidic chip.

Abstract: The various embodiments disclosed herein utilize multiple lasers that have different wavelengths and a single detection path. The lasers are mounted orthogonal to one another so that one laser will provide a forward angle light scatter (FALS) signal in the detection path, and one laser will provide a side scatter signal in the detection path (i.e., the single detection optics are approximately in-line with the FALS laser and approximately orthogonal to the side scatter laser). The single detector path spectrally separates the forward and side scatter signals prior to applying them to their respective detectors for measurement.

Abstract: A system and method are disclosed in which particles sorted by a flow cytometer may be deposited directly into a deposition layer formed on the surface of an optical disc, and information regarding measurements made of the particle, as well as the storage location of the particle, can be written to the recording layer of the optical disc.

Abstract: The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices allow light collection from multiple directions. In certain other embodiments, the microfluidic devices use spatial intensity modulation. In certain other embodiments, the microfluidic devices have magnetic field separators. In certain other embodiments, the microfluidic devices have the ability to stack. In certain other embodiments, the microfluidic devices have 3-D hydrodynamic focusing to align sperm cells. In certain other embodiments, the microfluidic devices have acoustic energy couplers. In certain other embodiments, the microfluidic devices have phase variation producing lenses. In certain other embodiments, the microfluidic devices have transmissive and reflective lenses. In certain other embodiments, the microfluidic devices have integrally-formed optics.

Abstract: The present disclosure relates to microfluidic devices adapted for post-centrifugation use with selective sample extraction, and methods for their use. Certain embodiments make use of a dye-selective sample extraction. Other embodiments make use of a geographically-selective sample extraction. Other embodiments are also disclosed.

Abstract: The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices have onboard sterilization capabilities. In other embodiments, microfluidic devices have integral collection bags and methods for keeping the microfluidic channels clean.

Abstract: The present disclosure relates to microfluidic devices adapted for post-centrifugation use with selective sample extraction, and methods for their use. Certain embodiments make use of a dye-selective sample extraction. Other embodiments make use of a geographically-selective sample extraction. Other embodiments are also disclosed.

Abstract: A system and method are disclosed in which particles sorted by a flow cytometer may be deposited directly into a deposition layer formed on the surface of an optical disc, and information regarding measurements made of the particle, as well as the storage location of the particle, can be written to the recording layer of the optical disc.

Abstract: A sorting methodology in which bulk analysis of samples that have a low probability of containing a rare particle is performed first. Only those samples in which the bulk analysis has detected one or more rare particles are subjected to a flow cytometry process to isolate the rare particle(s). In one embodiment, a microwell plate provides for high throughput sorting in an enclosed environment. The microwell plate includes a plurality of wells and a microfluidic layer adapted for flow cytometry wherein particles from a given well are interrogated and sorted into other wells. The microfluidic layer advantageously includes fluid switches to permit bi-directional flow cytometry.

Abstract: The various embodiments disclosed herein utilize multiple lasers that have different wavelengths and a single detection path. The lasers are mounted orthogonal to one another so that one laser will provide a forward angle light scatter (FALS) signal in the detection path, and one laser will provide a side scatter signal in the detection path (i.e., the single detection optics are approximately in-line with the FALS laser and approximately orthogonal to the side scatter laser). The single detector path spectrally separates the forward and side scatter signals prior to applying them to their respective detectors for measurement.

Abstract: A sorting methodology in which bulk analysis of samples that have a low probability of containing a rare particle is performed first. Only those samples in which the bulk analysis has detected one or more rare particles are subjected to a flow cytometry process to isolate the rare particle(s). In one embodiment, a microwell plate provides for high throughput sorting in an enclosed environment. The microwell plate comprises a plurality of wells and a microfluidic layer adapted for flow cytometry wherein particles from a given well are interrogated and sorted into other wells. The microfluidic layer advantageously includes fluid switches to permit bi-directional flow cytometry.

Abstract: A high speed fluid switch is used to separate desired particles from a source stream containing particles, such as cells. A droplet generator creates and regulates a stream of droplets, which intersects with a source stream containing the particles to be sorted. The stream of droplets can be regulated such that portions of the source stream containing desired particles are deflected into a collection vessel, or such that portions of the source stream containing desired particles are permitted to pass uninterrupted. The high speed fluid switch may be implemented on a microfluidic chip.

Abstract: The present disclosure relates to microfluidic devices incorporating a gain medium, such as a laser gain medium, and methods for their use. Certain embodiments make use of mirrors integrated into the microfluidic device substrate. Other embodiments are also disclosed.

Abstract: The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices allow light collection from multiple directions. In certain other embodiments, the microfluidic devices use spatial intensity modulation. In certain other embodiments, the microfluidic devices have magnetic field separators. In certain other embodiments, the microfluidic devices have the ability to stack. In certain other embodiments, the microfluidic devices have 3-D hydrodynamic focusing to align sperm cells. In certain other embodiments, the microfluidic devices have acoustic energy couplers. In certain other embodiments, the microfluidic devices have phase variation producing lenses. In certain other embodiments, the microfluidic devices have transmissive and reflective lenses. In certain other embodiments, the microfluidic devices have integrally-formed optics.

Abstract: The present disclosure relates to microfluidic devices adapted for post-centrifugation use with selective sample extraction, and methods for their use. Certain embodiments make use of a dye-selective sample extraction. Other embodiments make use of a geographically-selective sample extraction. Other embodiments are also disclosed.

Abstract: A system and method for the measurement of multiple emissions in multiple flow channels in a flow cytometry system is disclosed where each excitation source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all sources in all flow channels, and the emissions are segregated using Fourier Transform techniques. The system and method are well-suited to microfluidic applications.

Abstract: The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices have onboard sterilization capabilities. In other embodiments, microfluidic devices have integral collection bags and methods for keeping the microfluidic channels clean.

Abstract: The present disclosure is generally directed to systems for the storage and preservation of an original tissue or cell sample onboard a microfluidic device, such as a cytometry chip. In some embodiments, the sample may be disassociated while onboard the microfluidic device.

Abstract: The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices have onboard data storage capabilities. In certain other embodiments, the microfluidic devices have onboard anticoagulants. In certain other embodiments, the microfluidic devices have onboard test and control channels. In certain other embodiments, the microfluidic devices have integrated collection media. In certain other embodiments, the microfluidic devices have multiple onboard test channels. In certain other embodiments, the microfluidic devices have localized temperature control. In certain other embodiments, the microfluidic devices have anatomy simulating regions. In certain other embodiments, the microfluidic devices have complete assay capabilities. In certain other embodiments, the microfluidic devices have dissociable sections.