Patents by Inventor Georg Thierbach

Georg Thierbach has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6822085
    Abstract: Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.
    Type: Grant
    Filed: September 26, 2001
    Date of Patent: November 23, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Natalie Schischka, Brigitte Bathe, Walter Pfefferle, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Patent number: 6815196
    Abstract: The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: November 9, 2004
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Patent number: 6812016
    Abstract: The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: November 2, 2004
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20040213874
    Abstract: The invention provides a process for the preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of microorganisms of the Bacillus group, in particular those which already produce D-pantothenic acid, which comprises enhancing, in particular over-expressing, in the microorganisms one or more of the nucleotide sequence(s) which code(s) for the gene or ORF ybbT, ywkA, yjmC, ytsJ, mdh, cysK, iolJ, pdhD, yuiE, dhaS, adk, yusH, yqhJ, yqhK and yqhI.
    Type: Application
    Filed: June 2, 2004
    Publication date: October 28, 2004
    Inventors: Daniela Kruse, Georg Thierbach
  • Publication number: 20040197878
    Abstract: The invention provides a process for the fermentative preparation of D-pantothenic acid and/or salts thereof or feed-stuffs additives comprising these by fermentation of microorganisms of the Bacillus group, in particular those which produce D-pantothenic acid, which comprises attenuating, eliminating or expressing at a low level in the microorganisms one or more of the nucleotide sequence(s) which code(s) for the gene or ORF azlC, azlD, ydaP and pckA or of the proteins coded by these.
    Type: Application
    Filed: May 18, 2004
    Publication date: October 7, 2004
    Inventors: Daniela Kruse, Georg Thierbach
  • Patent number: 6787334
    Abstract: The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: September 7, 2004
    Assignee: Degussa AG
    Inventors: Frank Elischweski, Jorn Kalinowski, Alfred Puhler, Nicole Dusch, Jurgen Dohmen, Mike Farwick, Georg Thierbach
  • Patent number: 6777229
    Abstract: This invention relates to the mutually compatible plasmids pTET3 and pCRY4, isolated from the strain of Corynebacterium glutamicum deposited under DSM number 5616, wherein plasmid pTET3 is characterised by 1.1 a length of ˜27.8 kbp and the restriction map shown in FIG. 1, 1.2 a replication region comprising the nucleotide sequence shown in SEQ ID no. 1 and 1.3 an antibiotic resistance region consisting of a tetA gene imparting tetracycline resistance and an aadA gene imparting streptomycin and spectinomycin resistance, shown in SEQ ID no. 6, and plasmid pCRY4 is characterised by 1.4 a length of ˜48 kbp and the restriction map shown in FIG. 2 and 1.5 a replication region comprising the nucleotide sequence shown in SEQ ID no. 4 to composite plasmid vectors of these plasmids which are capable of autonomous replication in coryneform bacteria and to processes for the production of L-amino acids, vitamins and nucleotides using these bacteria.
    Type: Grant
    Filed: November 3, 2000
    Date of Patent: August 17, 2004
    Assignee: Degussa AG
    Inventors: Andreas Tauch, Jorn Kalinowski, Alfred Puhler, Georg Thierbach
  • Publication number: 20040152173
    Abstract: The invention provides a process for the fermentative preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of microorganisms of the Bacillus group, in particular those which already produce D-pantothenic acid, which comprises enhancing, in particular over-expressing, in the microorganisms one or more of the nucleotide sequence(s) which code(s) for the gene or ORF serA, serc, ywpJ or glyA.
    Type: Application
    Filed: December 15, 2003
    Publication date: August 5, 2004
    Inventor: Georg Thierbach
  • Patent number: 6759224
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: July 6, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Jennifer Brehme, Walter Pfefferle, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20040043458
    Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: February 5, 2003
    Publication date: March 4, 2004
    Applicant: Degussa AG
    Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
  • Publication number: 20040038372
    Abstract: The invention relates to polynucleotide sequences of pgi genes encoding polypeptide sequences having phosphoglucose isomerase activity from coryneform bacteria, to coryneform bacteria containing such polynucleotides and/or polypeptides, a process for the production of L-amino acids using such polynucleotides and/or polypeptides, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of phosphoglucose isomerase activity.
    Type: Application
    Filed: May 8, 2003
    Publication date: February 26, 2004
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Achim Marx, Walter Pfefferle, Georg Thierbach
  • Patent number: 6692946
    Abstract: The present invention relates to polynucleotides corresponding to the nadA gene and which encode the quinolinate synthetase A protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having quinolinate synthetase A activity.
    Type: Grant
    Filed: February 22, 2001
    Date of Patent: February 17, 2004
    Assignee: Degussa AG
    Inventors: Christine Bastuck, Brigitte Bathe, Nicole Dusch, Bettina Moeckel, Georg Thierbach
  • Patent number: 6689587
    Abstract: The present invention relates to polynucleotides corresponding to the nadC gene and which encode the nicotinate nucleotide pyrophosphorylase protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having nicotinate nucleotide pyrophosphorylase activity.
    Type: Grant
    Filed: February 22, 2001
    Date of Patent: February 10, 2004
    Assignee: Degussa AG
    Inventors: Christine Bastuck, Brigitte Bathe, Nicole Dusch, Bettina Moeckel, Georg Thierbach
  • Patent number: 6667166
    Abstract: The present invention provides processes for preparing D-pantothenic acid using Coryneform bacteria having an enhanced pfkA gene.
    Type: Grant
    Filed: June 25, 2001
    Date of Patent: December 23, 2003
    Assignee: Degussa AG
    Inventors: Georg Thierbach, Nicole Dusch
  • Publication number: 20030199045
    Abstract: The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
    Type: Application
    Filed: March 6, 2002
    Publication date: October 23, 2003
    Inventors: Kevin Burke, Hermann Sahm, Lothar Eggeling, Bernd Moritz, L. K. Dunican, Ashling McCormack, Cliona Stapelton, Bettina Mockel, Georg Thierbach, Rita Dunican
  • Patent number: 6630332
    Abstract: The invention provides a process for the fermentative preparation of L-threonine using Enterobacteriaceae which in particular already produce L-threonine and in which the nucleotide sequence(s) which code(s) for the mqo gene are enhanced, in particular over-expressed.
    Type: Grant
    Filed: March 8, 2001
    Date of Patent: October 7, 2003
    Assignee: Degussa AG
    Inventors: Mechthild Rieping, Georg Thierbach, Michel Eduard Van Der Rest, Douwe Molenaar
  • Publication number: 20030175911
    Abstract: The invention relates to a process for the preparation of L-amino acids by the fermentation of coryneform bacteria. The process involves: fermenting an L-amino acid-producing bacteria in which at least the zwf gene is amplified; concentrating the L-amino acid in the medium or in the cells of the bacteria; and isolating the L-amino acid produced.
    Type: Application
    Filed: January 3, 2003
    Publication date: September 18, 2003
    Inventors: Stephen Hans, Brigitte Bathe, Alexander Reth, Georg Thierbach, Caroline Kreutzer, Bettina Mockel
  • Patent number: 6596516
    Abstract: A process for the preparation of L-amino acids, in which the following steps are carried out, a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is attenuated, in particular by removal of the natural promoter, and optionally b) concentrating the desired product in the medium or in the cells of the bacteria and c) isolating the L-amino acid, and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed, and nucleotide sequences of the lacI-tac-5′glyA or lacI-tac-glyA unit.
    Type: Grant
    Filed: December 8, 2000
    Date of Patent: July 22, 2003
    Assignees: Degussa AG, Forschungszentrum J├╝lich GmbH
    Inventors: Petra Ziegler, Lothar Eggeling, Hermann Sahm, Georg Thierbach, Walter Pfefferle
  • Patent number: 6582939
    Abstract: The preparation of alkaline earth salts of D-pantothenic acid, in which the fermentation is carried out in the presence of alkaline earth compounds.
    Type: Grant
    Filed: October 12, 2000
    Date of Patent: June 24, 2003
    Assignee: Degussa AG
    Inventors: Michael Binder, Walter Pfefferle, Ulrich Becker, IIona Walger, Klaus Erich Uffmann, Georg Thierbach, Achim Marx, Thomas Hermann
  • Publication number: 20030109014
    Abstract: The invention relates to a process for the preparation of L-amino acids by the fermentation of coryneform bacteria that over-express a gene encoding transketolase.
    Type: Application
    Filed: May 14, 2002
    Publication date: June 12, 2003
    Inventors: Kevin Burke, L. K. Dunican, Rita Duncian, Ashling McCormack, Cliona Stapleton, Bettina Mockel, Georg Thierbach