Patents by Inventor George G. Jokhadze

George G. Jokhadze has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230257735
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Application
    Filed: November 4, 2022
    Publication date: August 17, 2023
    Inventors: Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
  • Patent number: 11385226
    Abstract: An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample. In a homogenous assay, the method further comprises washing any such bound target. The bound target may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action on the substrate by the enzyme generates electrons, creating a potential across an anode and cathode which may be separated by a membrane.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: July 12, 2022
    Assignee: RED IVORY LLC
    Inventors: Marc Robert Labgold, George G. Jokhadze
  • Patent number: 11307201
    Abstract: An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample, and washing any such bound target. The bound target, if not immobilized, may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action of the enzyme upon the substrate frees electrons, creating a potential across an anode and cathode which may be separated by a membrane.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: April 19, 2022
    Assignee: RED IVORY LLC
    Inventors: Marc Robert Labgold, George G Jokhadze
  • Patent number: 11103831
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: August 31, 2021
    Assignee: Takara Bio USA, Inc.
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Patent number: 10954510
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: March 23, 2021
    Assignee: Takara Bio USA, Inc.
    Inventors: Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
  • Publication number: 20210002633
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Application
    Filed: August 13, 2020
    Publication date: January 7, 2021
    Inventors: Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
  • Publication number: 20200370095
    Abstract: The invention pertains to methods for assessing a section of a biological sample, e.g., for determining the location of an analyte in the section. In certain embodiments, the methods comprise: a) contacting the biological sample section with an oligonucleotide indexed surface comprising an addressable array of capture oligonucleotides; b) probing the oligonucleotide indexed surface contacted with the biological sample section with an analyte-specific binding member that specifically binds to the analyte, wherein the analyte-specific binding member comprises a detector oligonucleotide; c)linking the detector oligonucleotide to a barcoded capture oligonucleotide proximal thereto to produce a linked product nucleic acid, e.g., a ligated nucleic acid or an extension product nucleic acid; and d) sequencing the linked product nucleic acid to assess the biological sample for the analyte. Kits for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: May 22, 2020
    Publication date: November 26, 2020
    Inventors: Andrew Alan Farmer, George G. Jokhadze, Alain Mir
  • Patent number: 10781443
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: September 22, 2020
    Assignee: Takara Bio USA, Inc.
    Inventors: Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
  • Publication number: 20190111398
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Application
    Filed: December 12, 2018
    Publication date: April 18, 2019
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Patent number: 10195569
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: February 5, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Patent number: 9895665
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: February 20, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Publication number: 20180001266
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Application
    Filed: September 13, 2017
    Publication date: January 4, 2018
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Publication number: 20170198284
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Application
    Filed: March 24, 2017
    Publication date: July 13, 2017
    Inventors: Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
  • Publication number: 20170198285
    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
    Type: Application
    Filed: March 24, 2017
    Publication date: July 13, 2017
    Inventors: Craig Betts, Steve Oh, George G. Jokhadze, Nathalie Bolduc
  • Publication number: 20150238907
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Application
    Filed: December 23, 2014
    Publication date: August 27, 2015
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Publication number: 20150241417
    Abstract: Assay devices that include a poly(acid) membrane are provided. Aspects of the devices include a solid support and a poly(acid) membrane on a surface of the support, where the poly(acid) membrane includes an affinity element. In using the assay devices, a sample is contacted with the poly(acid) membrane and then a signal is obtained from the membrane. Also provided are kits that find use in practicing the methods described herein. The compositions and methods described herein find use in a variety of different applications, including analyte detection applications.
    Type: Application
    Filed: December 23, 2014
    Publication date: August 27, 2015
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Publication number: 20120269728
    Abstract: The present invention provides devices and methods for real time detection of target agents in a sample. These devices utilize tracking technology and selective binding to allow the identification of one or more target agents in a sample, and preferably in a biological sample. The present invention provides specific embodiments employing radio frequency identification devices.
    Type: Application
    Filed: June 21, 2007
    Publication date: October 25, 2012
    Applicant: Antara BioSciences Inc.
    Inventors: I-Min M. Jen, Mark O. Trulson, Kilian Dill, Marc R. Labgold, Dawei Sheng, George G. Jokhadze, Chandramohan V. Ammini, Peter Lobban
  • Publication number: 20090117574
    Abstract: An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample, and washing any such bound target. The bound target, if not immobilized, may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action of the enzyme upon the substrate frees electrons, creating a potential across an anode and cathode which may be separated by a membrane.
    Type: Application
    Filed: September 17, 2008
    Publication date: May 7, 2009
    Applicant: SIOMETRIX CORPORATION
    Inventors: MARC ROBERT LABGOLD, GEORGE G. JOKHADZE
  • Publication number: 20090107852
    Abstract: An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample. In a homogenous assay, the method further comprises washing any such bound target. The bound target may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action on the substrate by the enzyme generates electrons, creating a potential across an anode and cathode which may be separated by a membrane.
    Type: Application
    Filed: September 17, 2008
    Publication date: April 30, 2009
    Applicant: SIOMETRIX CORPORATION
    Inventors: Marc Robert Labgold, George G. Jokhadze
  • Publication number: 20090036315
    Abstract: The present invention provides a device and methods for the detection and quantification of one or more target agents in a sample by rapid and specific electrochemical detection. The present invention includes kits, devices and compositions capable of performing rapid, specific and accurate detection of one or more target agents in a sample.
    Type: Application
    Filed: February 7, 2007
    Publication date: February 5, 2009
    Applicant: Antara BioSciences Inc.
    Inventors: Marc R. Labgold, George G. Jokhadze, I-Min M. Jen, Naiping Shen, Mark T. Kozlowski, Chandramohan V. Ammini, David A. Suhy, Michael C. Norris, Peter E. Lobban