Patents by Inventor George M. Church

George M. Church has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230051627
    Abstract: The technology described herein is directed to methods for obtaining partial sequence information from a target protein. Also described herein are systems, devices, and kits for obtaining partial sequence information from a target protein.
    Type: Application
    Filed: August 1, 2022
    Publication date: February 16, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Mingjie DAI, George M. CHURCH, Marc W. KIRSCHNER
  • Patent number: 11566277
    Abstract: Methods of analyzing nucleic acids of a cell are provided.
    Type: Grant
    Filed: February 15, 2022
    Date of Patent: January 31, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Jehyuk Lee, Daniel Levner, Michael Super
  • Patent number: 11566276
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Grant
    Filed: July 2, 2021
    Date of Patent: January 31, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Jehyuk Lee, Daniel Levner, Michael Super
  • Publication number: 20230028318
    Abstract: Provided herein are compounds (i.e., fluorogenic amino acids (FgAAs), e.g., compounds of Formulae (I), (II), (III), and (IV)) that can be used in fluorescent labeling of biomolecules (e.g., proteins) and/or cells. Also described herein are methods of labeling and detecting biomolecules and/or cells by incorporating the FgAA compounds described herein into the biomolecules and/or cells (e.g., by enzymatic incorporation). Also provided herein are biomolecules, cells, compositions, and kits comprising the FgAA compounds described herein.
    Type: Application
    Filed: November 6, 2020
    Publication date: January 26, 2023
    Applicant: President and Fellows of Harvard College
    Inventors: Erkin Kuru, Jonathan Rittichier, George M. Church
  • Publication number: 20230022775
    Abstract: This disclosure provides riboregulators specific for particular viruses or for particular human transcription factors. The viral-specific riboregulators may be used to detect the presence of the particular virus, and this may enable diagnosis of an infection. The transcription factor specific riboregulators may be used to detect the presence and/or measure the level of the particular transcription factor, and this may enable diagnosis or prognosis of a particular condition such as cancer.
    Type: Application
    Filed: December 11, 2020
    Publication date: January 26, 2023
    Applicants: President and Fellows of Harvard College, Massachusetts Institute of Technology
    Inventors: Luis Ruben Soenksen Martinez, Nicolaas Angenent-Mari, Alexander S. Garruss, James J. Collins, George M. Church, Katherine Collins, Diogo M. Camacho
  • Publication number: 20230024183
    Abstract: Methods are provided for treating osteoarthritis by administering ?Klotho protein and sTGF?-R2 protein to a site within a mammal exhibiting symptoms of osteoarthritis, such as a knee joint. The ?Klotho protein and the sTGF?-R2 protein are both present at the osteoarthritic site.
    Type: Application
    Filed: December 4, 2020
    Publication date: January 26, 2023
    Inventors: Paloma Martinez Redondo, Isabel Guillen-Guillen, Juan Carlos Izpisua Belmonte, Noah Davidsohn, George M. Church, Pedro Guillen Garcia
  • Patent number: 11549136
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Grant
    Filed: July 2, 2021
    Date of Patent: January 10, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Jehyuk Lee, Daniel Levner, Michael Super
  • Publication number: 20230002451
    Abstract: The technology described herein provides variant adeno-associated viral capsid polypeptides and viruses comprising the same. Further provided herein are methods for delivering a viral payload using viruses comprising variant capsid polypeptides described herein.
    Type: Application
    Filed: November 6, 2020
    Publication date: January 5, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Eric KELSIC, Sam SINAI, Pierce OGDEN, George M. CHURCH
  • Patent number: 11542554
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Grant
    Filed: August 6, 2021
    Date of Patent: January 3, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-Hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Patent number: 11535863
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Grant
    Filed: June 13, 2019
    Date of Patent: December 27, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Prashant G. Mali, Luhan Yang
  • Patent number: 11532380
    Abstract: A method of storing information using monomers such as nucleotides is provided including converting a format of information into a plurality of bit sequences of a bit stream with each having a corresponding bit barcode, converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding, synthesizing the plurality of corresponding oligonucleotide sequences on a substrate having a plurality of reaction locations, and storing the synthesized plurality of corresponding oligonucleotide sequences.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: December 20, 2022
    Assignee: President and Fellows of Harvard College
    Inventor: George M. Church
  • Publication number: 20220396808
    Abstract: The technology described herein provides variant adeno-associated viral capsid polypeptides and viruses comprising the same. Further provided herein are methods for delivering a viral payload using viruses comprising variant capsid polypeptides described herein. Described herein are viral vectors comprising a variant sequence of the capsid gene, VP1. In particular, viral vectors with capsid polypeptide mutations that modify tropism of the viral particles relative to particles with wild-type capsid polypeptide are described.
    Type: Application
    Filed: November 6, 2020
    Publication date: December 15, 2022
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Eric KELSIC, Sam SINAI, George M. CHURCH
  • Publication number: 20220380811
    Abstract: Methods of multiplex genome engineering in cells using Cas9 is provided which includes a cycle of steps of introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to the target DNA and which guide the enzyme to the target DNA, wherein the one or more RNAs and the enzyme are members of a co-localization complex for the target DNA, and introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, and wherein the cycle is repeated a desired number of times to multiplex DNA engineering in cells.
    Type: Application
    Filed: June 10, 2022
    Publication date: December 1, 2022
    Inventors: George M. Church, James Dicarlo
  • Patent number: 11512325
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Grant
    Filed: February 16, 2022
    Date of Patent: November 29, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Prashant G. Mali, Luhan Yang
  • Patent number: 11473139
    Abstract: A method of sequencing nucleic acids is provided using DNA origami as a barcode for a nucleic acid probe.
    Type: Grant
    Filed: March 26, 2020
    Date of Patent: October 18, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault
  • Patent number: 11459586
    Abstract: Inhibiting p53 or Bax can be used to improve nuclease-mediated gene targeting frequencies in stem cells. This inhibition can be achieved, e.g., by overexpression of anti-apoptosis proteins or by silencing or reducing p53 or Bax expression. This technique can be used in conjunction with other rapidly developing CRISPR technologies, including improvements in specificity, other types of nucleases, and further enrichment, screening, and selection schemes, to expand the use of stem cells in experimental studies and tissue engineering for therapeutic purposes.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: October 4, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Susan M. Byrne
  • Patent number: 11459585
    Abstract: Methods of multiplex genome engineering in cells using Cas9 is provided which includes a cycle of steps of introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to the target DNA and which guide the enzyme to the target DNA, wherein the one or more RNAs and the enzyme are members of a co-localization complex for the target DNA, and introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, and wherein the cycle is repeated a desired number of times to multiplex DNA engineering in cells.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: October 4, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, James Dicarlo
  • Publication number: 20220309092
    Abstract: Methods, systems, and computer-readable media for processing spatially related sequence data received from a sequencing device are presented. In one or more embodiments, a computing platform may receive, from a sequencing device, image data associated with a sample. The computing platform may identify, based on the image data received from the sequencing device, a first sequence located at first spatial coordinates. Subsequently, the computing platform may store, in a spatially searchable database, a first data element comprising the first spatial coordinates and a first identifier corresponding to the first sequence to spatially relate the first sequence to other sequences present in the sample.
    Type: Application
    Filed: May 27, 2022
    Publication date: September 29, 2022
    Inventors: Evan R. Daugharthy, Vivek Dasari, George M. Church
  • Patent number: 11447807
    Abstract: The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: September 20, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry
  • Publication number: 20220282301
    Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.
    Type: Application
    Filed: May 19, 2022
    Publication date: September 8, 2022
    Inventors: Daniel Levner, Je-hyuk Lee, George M. Church, Michael Super