Abstract: The present invention provides a method for the targeted insertion of a nucleotide of interest into a specific chromosomal site within a plant cell. The method comprises the steps of: (a) providing a plant cell, the plant cell optionally but preferably having a heterologous target site on a chromosome thereof, wherein said target site is flanked by at least one recombination site; and then (b) transforming said plant cell with a transformation vector (e.g., with an Agrobacterium transformation vector) carrying a nucleotide sequence of interest, wherein said nucleotide sequence of interest is flanked by at least one recombination site that corresponds to the recombination sites of said target site, so that said nucleotide of interest is inserted into said chromosome at said target site (when a target site is employed).

Abstract: A method for introducing a heterologous nucleic acid of interest into a plant to thereby produce a recombinant plant is carried out by (a) providing a recombinant nucleic acid of interest, the recombinant nucleic acid comprising the heterologous nucleic acid of interest, and preferably including (i) a matrix attachment region (MAR) positioned 5′ to the heterologous DNA, (ii) a MAR positioned 3′ to the heterologous nucleic acid of interest, or (iii) a MAR positioned 5′ to the heterologous nucleic acid of interest and a MAR positioned 3′ to the heterologous nucleic acid of interest; (b) providing meristem tissue of the plant of interest; (c) introducing the recombinant nucleic acid of interest into the meristem tissue; and then (d) propagating a recombinant plant from the meristem tissue, preferably by a direct propagation technique.

Abstract: A method for introducing a heterologous nucleic acid of interest into a plant to thereby produce a recombinant plant is carried out by (a) providing a recombinant nucleic acid of interest, the recombinant nucleic acid comprising the heterologous nucleic acid of interest, and preferably including (i) a matrix attachment region (MAR) positioned 5′ to the heterologous DNA, (ii) a MAR positioned 3′ to the heterologous nucleic acid of interest, or (iii) a MAR positioned 5′ to the heterologous nucleic acid of interest and a MAR positioned 3′ to the heterologous nucleic acid of interest; (b) providing meristem tissue of the plant of interest; (c) introducing the recombinant nucleic acid of interest into the meristem tissue; and then (d) propagating a recombinant plant from the meristem tissue, preferably by a direct propagation technique.