Abstract: The invention relates to (1) pressure-mediated dissociation of an analyte complexed with an endogenous binding partner to enable detection of a complex formed from the analyte and an exogenous binding factor, (2) pressure-mediated association of an analyte and an exogenous binding partner to enable more rapid and/or more sensitive detection of an analyte, and (3) pressure-mediated association and dissociation of biomolecular complexes to enable separation of one biomolecule from a complex mixture. Pressure can be used to improve assays by dissociating endogenous analyte complexes and improving assay speed and sensitivity by associating the analyte molecules with exogenously supplied binding partners. Pressure can also be used to improve the separation of compounds from contaminated mixtures.

Abstract: The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system.In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.

Abstract: A substrate for detecting peroxidase activity in a peroxidase-based assay which includes a substantially aqueous solution of at least a 1-naphthol derivative and tetramethylbenzidine is described.

Abstract: The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.

Abstract: Apparatus for indicating the presence of a liquid on a member includes a substrate having a pattern thereon that is perceptibly visually modified by the presence of a liquid on the member.

Abstract: A process of forming indicia on autoradiograms is disclosed wherein a substrate containing a distribution of radioactive material, such as radioactively tagged proteins, is marked with a hexagonal Wurtzite form of zinc sulfide doped with trace metals phosphor. The thusly marked substrate is exposed to actinic radiation to charge the phosphor. The substrate is then superposed on an X-ray film to form a latent photographic image in the film which is developed by conventional means to form a photographic image of the phosphorescent indicia and the distribution of radioactive material in the substrate.

Abstract: An improved solid-phase radioimmunological assay method for assay of an antigen in a biological fluid, e.g., blood plasma, by use of a solid phase polymeric substrate on which is adsorbed in solid phase an antibody composite layer, comprising a solid phase precoat of an anti-antibody (Ab.sup.2) (i.e., an antibody to an animal blood serum and sometimes referred to as a second antibody) bound to said substrate and a solid phase antibody Ab specific to said antigen (sometimes referred to as a primary antibody) immunologically bound to said anti-antibody precoat.