Patents by Inventor Giuliano Galli

Giuliano Galli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6566105
    Abstract: A process is described for the production of D-&agr;-amino acids by the stereospecific conversion of racemic mixtures of 5-substituted hydantoins using a microorganism transformed with a plasmid which expresses an enzymatic system which converts the hydantoins into the corresponding D-&agr;-amino acids without the requirement for inducers. Also described is a plasmid containing genes encoding the enzymatic system, and a microorganism transformed with the plasmid.
    Type: Grant
    Filed: April 3, 1995
    Date of Patent: May 20, 2003
    Assignee: Eniricerche S.p.A.
    Inventors: Renata Grifantini, Gianni Frascotti, Giuliano Galli, Guido Grandi
  • Patent number: 5877002
    Abstract: The present invention relates to thermostable mutants of Agrobacterium radiobacter D-N-.alpha.-carbamoylase and means and methods for their preparation. The thermostable mutants have an unaltered or improved activity with respect to the wild-type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids.
    Type: Grant
    Filed: December 9, 1996
    Date of Patent: March 2, 1999
    Assignee: Eniricerche S.p.A.
    Inventors: Renata Grifantini, Giovanna Carpani, Giuliano Galli, Guido Grandi
  • Patent number: 5877003
    Abstract: The present invention relates to thermostable mutants of D-N-.alpha.-carbamoylase and means and methods for their preparation. The thermostable mutants have an unaltered or improved activity with respect to the wild-type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids.
    Type: Grant
    Filed: December 30, 1997
    Date of Patent: March 2, 1999
    Assignee: Eniricerche S.p.A
    Inventors: Renata Grifantini, Giovanna Carpani, Giuliano Galli, Guido Grandi
  • Patent number: 5869298
    Abstract: The present invention relates to mutants of D-N-.alpha.-carbamylase wherein at least one of the cysteines in position 243, 250 and 279 of the amino acid sequence of the wild type enzyme is substituted with a different residue selected from natural amino acids; a recombinant plasmid comprising a nucleotidic sequence which encodes for at least one of the mutants of D-N-.alpha.-carbamylase, host microorganisms transformed with said plasmid and a process for the preparation of these mutants by the culture of said microorganisms. The mutants of D-N-.alpha.-carbamylase have a higher enzymatic stability than that of the wild type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids providing an improvement in the production yields.
    Type: Grant
    Filed: April 3, 1995
    Date of Patent: February 9, 1999
    Assignee: Eniricerche S.p.A.
    Inventors: Giuliano Galli, Renata Grifantini, Guido Grandi
  • Patent number: 5869278
    Abstract: Mature Human Growth Hormone is prepared by enzymatic hydrolysis of a soluble precursor with immobilized Factor Xa. The soluble precursor contains a cleavage recognition sequence, Ile--Glu--Gly--Arg, for Factor Xa. The Factor Xa is preferably immobilized on CNBr activated Sepharose Cl-4B containing cyclic imido carbonate groups. Immobilization is carried out by reaction of amine groups on Factor X with the cyclic imido carbonate groups of the CNBr activated Sepharose Cl-4B to form covalently immobilized Factor X, which is then activated to Factor Xa with a protein contained in Russell's viper venom (RVV) in the presence of Ca.sup.++ ions.
    Type: Grant
    Filed: February 28, 1994
    Date of Patent: February 9, 1999
    Assignee: Eniricerche S.p.A.
    Inventors: Guido Grandi, Giuliano Galli
  • Patent number: 5834258
    Abstract: In an improved process for the preparation of D-.alpha.-amino acids by the stereoselective conversion of racemic mixtures of 5-substituted hydantoins with an enzymatic system produced by a microorganism, the improvement consists in the fact that a microorganism is used which is transformed with the plasmid pSM700 cultivated at a temperature of between 20.degree. C. and 28.degree. C. The use of this microorganism allows an improvement in the expression of the enzymatic system and an increase in the conversion rate of the racemic hydantoin to D-.alpha.-amino acid.
    Type: Grant
    Filed: November 6, 1996
    Date of Patent: November 10, 1998
    Assignee: Eniricerche S.p.A.
    Inventors: Renata Grifantini, Giuliano Galli, Giovanna Carpani, Guido Grandi
  • Patent number: 5807710
    Abstract: The present invention relates to mutants of D-N-.alpha.-carbamylase wherein at least one of the cysteines in position 243, 250 and 279 of the amino acid sequence of the wild type enzyme is substituted with a different residue selected from natural amino acids; a recombinant plasmid comprising a nucleotidic sequence which encodes for at least one of the mutants of D-N-.alpha.-carbamylase, host microorganisms transformed with said plasmid and a process for the preparation of these mutants by the culture of said microorganisms. The mutants of D-N-.alpha.-carbamoylase have a higher enzymatic stability than that of the wild type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids providing an improvement in the production yields.
    Type: Grant
    Filed: July 25, 1997
    Date of Patent: September 15, 1998
    Assignee: Eniricerche S.p.A.
    Inventors: Giuliano Galli, Renata Grifantini, Guido Grandi
  • Patent number: 5728571
    Abstract: A new plasmid vector is described which can replicate in B. subtilis and which can express and secrete, in the culture medium, human beta interleukin-1 (beta IL-1) without amino acid sequences extraneous to the natural molecule, a strain of B. subtilis transformed by the vector and a method for the expression and secretion of mature human beta interleukin-1. The human beta interleukin-1 thus obtained shows a specific acitvity of 1.times.10.sup.9 U/mg of protein and is particularly suitable as a stimulant for the immune system, as an adjuvant in vaccines for the activation of the repair mechanism in cases of tissue damage, and for the treatment of auto-immune diseases in man.
    Type: Grant
    Filed: March 17, 1995
    Date of Patent: March 17, 1998
    Assignee: Eniricerche S.p.A.
    Inventors: Ada Velati Bellini, Giuliano Galli, Giuseppe Lucchese, Guido Grandi
  • Patent number: 5652116
    Abstract: Disclosed is a novel method for the in vivo and in vitro synthesis of modified peptides, using either engineered microorganisms capable of expressing peptide synthetases with modified substrate specificity, or peptide synthetases extracted from said microorganisms.
    Type: Grant
    Filed: July 6, 1994
    Date of Patent: July 29, 1997
    Assignee: Eniricerche S.p.A.
    Inventors: Guido Grandi, Francesca De Ferra, Giuliano Galli, Paola Cosmina
  • Patent number: 5457037
    Abstract: The structural gene encoding isoamylase from Pseudomonas SMP 1 is obtained. Also described are vectors comprising the isoamylase gene and host microorganisms transformed by these vectors. The transformed host microorganisms are capable of expressing and/or secreting mature isoamylase having biological activity.
    Type: Grant
    Filed: January 8, 1993
    Date of Patent: October 10, 1995
    Assignee: Enichem Synthesis, S.p.A.
    Inventors: Angelo Tognoni, Paolo Carrera, Barbara Camerini, Giuliano Galli, Giuseppe Lucchese, Guido Grandi, Carlo Di Gennaro
  • Patent number: 5437988
    Abstract: A new plasmid vector is described which can replicate in B.subtilis and which can express and secrete, in the culture medium, human beta interleukin-1 (beta IL-1) without amino acid sequences extraneous to the natural molecule, a strain of B.subtilis transformed by the vector and a method for the expression and secretion of mature human beta interleukin-1. The human beta interleukin-1 thus obtained shows a specific acitvity of 1.times.10.sup.9 U/mg of protein and is particularly suitable as a stimulant for the immune system, as an adjuvant in vaccines for the activation of the repair mechanism in cases of tissue damage, and for the treatment of auto-immune diseases in man.
    Type: Grant
    Filed: June 7, 1993
    Date of Patent: August 1, 1995
    Assignee: Eniricerche S.p.A.
    Inventors: Ada V. Bellini, Giuliano Galli, Giuseppe Lucchese, Guido Grandi
  • Patent number: 4065353
    Abstract: An enzymatic hydrolysis method is disclosed for the preparation of D-carbamyl aminoacids and the corresponding D-aminoacids.The method is based on the use of certain substituted hydantoins as the starting materials and these are hydrolized, in a temperature range from 10.degree. C to 70.degree. C, with hydropyrimidine hydrolase from calf livers. The pH range is from 8 to 9.The method in question is cheaper and more reliable than those of the prior art.
    Type: Grant
    Filed: May 11, 1976
    Date of Patent: December 27, 1977
    Assignee: Snamprogetti, S.p.A.
    Inventors: Francesco Cecere, Giuliano Galli, Gino Della Penna, Bruno Rappuoli