Abstract: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the conformal layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the matrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of PoIµ, PoI?, PoI?, and PoI? of any species or the homologous amino acid sequence of X family polymerases of any species in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases or homologous enzymes and 3?-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.

Abstract: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

Abstract: Disclosed an improved process for synthesising a nucleic acid strand using cycles of template independent enzyme extension. Having one or more of the amino groups on the base heterocyclic groups masked with protecting groups helps to prevent secondary structure in the extended strand, thereby improving access of the enzyme to the 3? OH terminus for extension.

Abstract: The invention provided herein relates to methods for protein synthesis, characterisation and purification on a digital microfluidic device.

Abstract: The present invention relates to a compound according to Formula (1c) or (1d): wherein, R1; R2; R3 and R6 are defined herein, and their use in methods of nucleic acid synthesis.

Abstract: The invention relates to improved methods of enzymatic solid-supported nucleic acid synthesis that make use of terminal deoxynucleotidyl transferase (TdT) enzymes or modified terminal deoxynucleotidyl transferase (TdT) enzymes on polyacrylamide type supports. The invention further relates to the use of kits comprising said enzymes in a method of solid-supported nucleic acid synthesis.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: A play apparatus has a pair of devices. Each device has: a capture arrangement such as a cavity to capture an object such as a ball; an object detector to detect that the object has been captured; a transmission module to transmit a release signal; a receiver module to receive a release signal; and a release actuator to release the captured object. A first device detects capture of a first object, transmits a first release signal to cause a second device to release a second object. The first device withholds the first object until releasing it responsive to receipt of a second release signal indicating that another object has been captured by the second device.

Abstract: The invention relates to methods and kits for the synthesis and assembly of oligonucleotides into contiguous strands. The oligonucleotides can be synthesised and assembled in the same device, allowing production of strands longer than can be prepared using base by base synthesis alone.

Abstract: A computerized system manages health documents in a computer database. Each document in the database is associated with an author, which is in turn associated with group. When documents for a patient are listed for a user, the computerized system uses the user's own group assignment within the database to organize and present the patient documents. Rules associated with the user's group create ad hoc categories of documents that are of special interest to the user.

Abstract: The invention relates to methods and kits for the synthesis of oligonucleotides via controlled, localised deprotection of 3?-ONH2 groups on a solid support.

Abstract: A computerized system manages health documents in a computer database. Each document in the database is associated with an author, which is in turn associated with group. When documents for a patient are listed for a user, the computerized system uses the user's own group assignment within the database to organize and present the patient documents. Rules associated with the user's group create ad hoc categories of documents that are of special interest to the user.

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Pol?, Pol?, Pol?, and Pol? of any species or the homologous amino acid sequence of X family polymerases of any species and uses thereof.

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Pol?, Pol?, Pol?, and Pol? of any species or the homologous amino acid sequence of X family polymerases of any species and their use in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of new terminal deoxynucleotidyl transferases and 3?-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.

Abstract: A play apparatus has a pair of devices. Each device has: a capture arrangement such as a cavity to capture an object such as a ball; an object detector to detect that the object has been captured; a transmission module to transmit a release signal; a receiver module to receive a release signal; and a release actuator to release the captured object. A first device detects capture of a first object, transmits a first release signal to cause a second device to release a second object. The first device withholds the first object until releasing it responsive to receipt of a second release signal indicating that another object has been captured by the second device.

Abstract: A computerized system manages health documents in a computer database. Each document in the database is associated with an author, which is in turn associated with group. When documents for a patient are listed for a user, the computerized system uses the user's own group assignment within the database to organize and present the patient documents. Rules associated with the user's group create ad hoc categories of documents that are of special interest to the user.