Abstract: The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system and to methods for monitoring real-time PCR in such systems.

Abstract: A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.

Abstract: A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.

Abstract: A system includes a first database to associate a sub-interface identifier with customer information, a second database to store a history of customer usage records, and a first device. The first device may receive information from a routing device, where the information includes the sub-interface identifier for a sub-interface of the routing device and information identifying an amount of network traffic received at or transmitted from the sub-interface over a time period, use the sub-interface identifier to obtain the associated customer information from the first database, associate the customer information with the information identifying an amount of network traffic received at or transmitted from the sub-interface over a time period to create a new customer usage record, and store the new customer usage record in the second database.

Abstract: Disclosed herein is a process of reducing the ash content of a biomass feedstock or a biomass fraction. Embodiments include a process of reducing the ash content of a biomass feedstock or of a biomass fraction comprising: providing a biomass or biomass fraction; adding alcohol and acid to the biomass to yield a reaction mixture; separating the reaction mixture into a solid fraction and a liquid fraction by centrifugation; and distillating the liquid and collecting the solid to recover reaction reagents. The biomass can comprise aquatic species such as lemna.

Abstract: The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. The present invention in one embodiment provides a system that includes a microfluidic channel comprising a PCR processing zone and an HRTm analysis zone; and an image sensor having a first image sensor region having a first field of view and a second image sensor region having a second field of view, wherein the second field of view is different than the first field of view, wherein at least a portion of the PCR processing zone is within the first field of view; and at least a portion of the HRTm analysis zone is within the second field of view.

Abstract: In one aspect, the present invention provides a systems and methods for the real-time amplification and analysis of a sample of DNA.

Abstract: A system includes a first database to associate a sub-interface identifier with customer information, a second database to store a history of customer usage records, and a first device. The first device may receive information from a routing device, where the information includes the sub-interface identifier for a sub-interface of the routing device and information identifying an amount of network traffic received at or transmitted from the sub-interface over a time period, use the sub-interface identifier to obtain the associated customer information from the first database, associate the customer information with the information identifying an amount of network traffic received at or transmitted from the sub-interface over a time period to create a new customer usage record, and store the new customer usage record in the second database.

Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.

Abstract: A state storage application can be configured to retrieve, from a computing device coupled to a host computing system, state information for the computing device, with the state information reflecting a current or past state of the computing device. The state storage application can be further configured to store the retrieved state information in a storage medium. A monitoring application can be configured to access the stored state information, and send at least a portion of the accessed state information to one or more servers.

Abstract: The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.

Abstract: At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.

Abstract: The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. The present invention in one embodiment provides a system that includes a microfluidic channel comprising a PCR processing zone and an HRTm analysis zone; and an image sensor having a first image sensor region having a first field of view and a second image sensor region having a second field of view, wherein the second field of view is different than the first field of view, wherein at least a portion of the PCR processing zone is within the first field of view; and at least a portion of the HRTm analysis zone is within the second field of view.

Abstract: An energy recovery apparatus for mounting on a structure to recover energy from air movement caused by vehicles passing under the structure is disclosed. The energy recovery apparatus may include a support assembly for mounting on the structure, and a rotating assembly rotatabaly mounted on the support assembly. The rotating assembly may include a rotating frame and a plurality of blades mounted on the frame such that the blades are free to be rotated by air movement caused by vehicle movement adjacent to the structure when the apparatus is mounted on the structure. The apparatus may also include a generator operatively connected to the rotating assembly to be driven by rotation of the rotating assembly, and to generate electrical power when rotated by connection to the rotating assembly.

Abstract: The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.

Abstract: A first list includes a first set of destination names. The first set of destination names including destination names involved in network communications engaged in by a device.

Abstract: The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system.

Abstract: A system for amplifying nucleic acids is disclosed which, in one embodiment, includes a fluidic device having a sample channel and a heat exchange channel disposed sufficiently close to the sample channel such that a heat exchange fluid in the heat exchange channel can cause a sample in the sample channel to gain or lose heat at desired levels. In one illustrative embodiment, the system further includes three reservoirs coupled to the heat exchange channel and a temperature control system configured to heat fluids stored in the respective reservoirs at different temperatures. One or more pumps and a controller are configured to cause fluid stored in the reservoirs to enter and flow through the heat exchange channel at different times.

Abstract: The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system and to methods for monitoring real-time PCR in such systems.

Abstract: A system and method for controlling fluid flow within a microchannel includes a fluid circuit comprising a fluid outlet well and one or more fluid inlet wells, all in communication with a microchannel. A negative pressure differential is applied to the outlet well and fluid flow from an inlet well into the microchannel is controlled by opening or closing the inlet well to atmospheric pressure. To stop fluid flow from the inlet well, a negative pressure differential may be applied to the inlet well to equalize pressure between the inlet and outlet wells. By sequentially opening and closing different inlet wells to atmosphere, controlled amounts of different reagents can be serially introduced into the microchannel.