Patents by Inventor Gregory N. Stephanopoulos

Gregory N. Stephanopoulos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190330642
    Abstract: The invention provides, inter alia, a nucleic acid (e.g. expression vector) that comprises at least a first coding sequence and a second coding sequence. Each conding sequence is under the control of an inducible promoter of defined strength. Different promoters can have different strengths. Each promoter is responsive to the same inducer. The invention also provides: methods of expressing coding regions, methods of making a product of a multi-enzyme pathway, and methods of optimizing the yield of a product of a multi-enzyme metabolic pathway using the nucleic acids provided by the invention. Also disclosed is a method of non-enzymatic gene cloning useful for practicing the invention.
    Type: Application
    Filed: June 20, 2019
    Publication date: October 31, 2019
    Inventors: Heng Phon Too, Ruiyang Zou, Gregory N. Stephanopoulos
  • Patent number: 9193853
    Abstract: A method for microbial and/or enzymatic devulcanization of rubber includes: providing a particulate vulcanized rubber compound, swelling the vulcanized rubber compound in a solvent, and introducing a microbe or enzyme into the solvent containing the particulate vulcanized rubber in order to devulcanize the rubber compound.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: November 24, 2015
    Assignee: APPIA, LLC
    Inventors: Georg G. Bohm, Gregory N. Stephanopoulos
  • Publication number: 20150284729
    Abstract: The invention provides, inter alia, a nucleic acid (e.g. expression vector) that comprises at least a first coding sequence and a second coding sequence. Each conding sequence is under the control of an inducible promoter of defined strength. Different promoters can have different strengths. Each promoter is responsive to the same inducer. The invention also provides: methods of expressing coding regions, methods of making a product of a multi-enzyme pathway, and methods of optimizing the yield of a product of a multi-enzyme metabolic pathway using the nucleic acids provided by the invention. Also disclosed is a method of non-enzymatic gene cloning useful for practicing the invention.
    Type: Application
    Filed: November 15, 2013
    Publication date: October 8, 2015
    Inventors: Heng Phon Too, Ruiyang Zou, Gregory N. Stephanopoulos
  • Publication number: 20110301302
    Abstract: A method for microbial and/or enzymatic devulcanization of rubber includes: providing a particulate vulcanized rubber compound, swelling the vulcanized rubber compound in a solvent, and introducing a microbe or enzyme into the solvent containing the particulate vulcanized rubber in order to devulcanize the rubber compound.
    Type: Application
    Filed: May 26, 2011
    Publication date: December 8, 2011
    Inventors: Georg G. Bohm, Gregory N. Stephanopoulos
  • Publication number: 20090011493
    Abstract: The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
    Type: Application
    Filed: July 1, 2008
    Publication date: January 8, 2009
    Applicant: President and Fellows of Harvard College
    Inventors: Rahul Singhvi, Amit Kumar, George M. Whitesides, Donald E. Ingber, Gabriel P. Lopez, Daniel I. C. Wang, Gregory N. Stephanopoulos
  • Patent number: 7067306
    Abstract: The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: June 27, 2006
    Assignee: President & Fellows of Harvard College
    Inventors: Rahul Singhvi, Amit Kumar, George M. Whitesides, Donald E. Ingber, Gabriel P. Lopez, Daniel I. C. Wang, Gregory N. Stephanopoulos
  • Publication number: 20020094572
    Abstract: The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
    Type: Application
    Filed: December 21, 2001
    Publication date: July 18, 2002
    Inventors: Rahul Singhvi, Amit Kumar, George M. Whitesides, Donald E. Ingber, Gabriel P. Lopez, Daniel I. C. Wang, Gregory N. Stephanopoulos
  • Patent number: 6368838
    Abstract: Methods and a device are provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere. The cytophobic regions can be wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. Further, the islands or the regions or both may be formed of a self-assembled monolayer (SAM). The methods are for forming a patterned surface for a population of cells and for selectively manipulating individual cells formed on the surface of the plate of the device. Furthermore, the device is used in immobilization of cells at a surface and for controlling shape of the cells.
    Type: Grant
    Filed: August 12, 1999
    Date of Patent: April 9, 2002
    Assignee: President and Fellows of Havard College
    Inventors: Rahul Singhvi, Amit Kumar, George M. Whitesides, Donald E. Ingber, Gabriel P. Lopez, Daniel I. C. Wang, Gregory N. Stephanopoulos
  • Patent number: 5976826
    Abstract: A device is provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.
    Type: Grant
    Filed: October 16, 1997
    Date of Patent: November 2, 1999
    Assignees: President and Fellows of Harvard College, Massachusetts Institute of Technology, Children's Medical Center Corporation
    Inventors: Rahul Singhvi, Amit Kumar, George M. Whitesides, Donald E. Ingber, Gabriel P. Lopez, Daniel I. C. Wang, Gregory N. Stephanopoulos
  • Patent number: 5776748
    Abstract: The disclosed invention is a device for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.
    Type: Grant
    Filed: June 6, 1996
    Date of Patent: July 7, 1998
    Assignees: President and Fellows of Harvard College, Massachusetts Institute of Technology, Children's Medical Center Corporation
    Inventors: Rahul Singhvi, Amit Kumar, George M. Whitesides, Donald E. Ingber, Gabriel P. Lopez, Daniel I. C. Wang, Gregory N. Stephanopoulos
  • Patent number: 4969741
    Abstract: This invention pertains to a method and an apparatus for determining concentration of solid particles of interest in a sample in the presence of at least one other type of solid particle by measuring light scatter at a wavelength which is independent of solid particle concentration which is not of interest and related to solid particle concentration of interest. Preferably, solid particles of interest are cells grown in cell culture medium comprising a solid substrate.
    Type: Grant
    Filed: July 21, 1989
    Date of Patent: November 13, 1990
    Assignee: Massachusetts Institute of Technology
    Inventors: Max J. Kennedy, Daniel I. C. Wang, Gregory N. Stephanopoulos