Patents by Inventor Gregory Paul Winter
Gregory Paul Winter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6495673Abstract: A method for isolating the DNA encoding an enzyme is presented. The DNA is contained within a support to which the enzyme is linked, wherein the enzyme is further linked to a substrate. When the enzyme reacts with the substrate, the product remains linked to the enzyme. The product linked to the enzyme which is linked to the support containing the DNA encoding the enzyme is isolated, thereby isolating the DNA encoding the enzyme. In this manner an expression library of enzymes produced by mutagenesis can be screened for mutated active enzyme, and thereby obtain the mutated DNA encoding the mutated active enzyme.Type: GrantFiled: October 30, 2000Date of Patent: December 17, 2002Assignee: Medical Research CouncilInventors: Dario Neri, Salvatore Demartis, Adrian Huber, Francesca Viti, Dan S Tawfik, Gregory Paul Winter
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Patent number: 6492123Abstract: Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.Type: GrantFiled: September 3, 1998Date of Patent: December 10, 2002Assignee: Medical Research CouncilInventors: Kaspar-Philip Holliger, Andrew David Griffiths, Hendricus Renerus Jacobus Matheus Hoogenboom, Magnus Malmqvist, James David Marks, Brian Timothy McGuinness, Anthony Richard Pope, Terence Derek Prospero, Gregory Paul Winter
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Patent number: 6492160Abstract: Methods, recombinant host cells and kits are disclosed-for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.Type: GrantFiled: June 25, 1998Date of Patent: December 10, 2002Assignees: Cambridge Antibody Technology Limited, Medical Research CouncilInventors: Andrew David Griffiths, Samuel Cameron Williams, Peter Michael Waterhouse, Ahuva Nissim, Gregory Paul Winter, Kevin Stuart Johnson, Andrew John Hammond Smith
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Patent number: 6316409Abstract: The present invention relates to ligands capable of binding a calcium dependent binding protein, that comprise an amino acid sequence corresponding to that of a wild type ligand for the calcium dependent binding protein with modification which results in enhanced affinity of the ligand for the calcium dependent binding protein.Type: GrantFiled: March 3, 1999Date of Patent: November 13, 2001Assignee: Medical Research CouncilInventors: Dario Neri, Gregory Paul Winter
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Patent number: 6291650Abstract: The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs “chain shuffling” in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.Type: GrantFiled: June 25, 1998Date of Patent: September 18, 2001Assignees: Cambridge Antibody Technology, Ltd., Medical Research CouncilInventors: Gregory Paul Winter, Kevin Stuart Johnson, Andrew David Griffiths, Andrew John Hammond Smith
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Patent number: 6248516Abstract: The present invention relates to single domain ligands derived from molecules in the immunoglobulin (Ig) superfamily, receptors comprising at least one such ligand, methods for cloning, amplifying and expressing DNA sequences encoding such ligands, preferably using the polymerase chain reaction, methods for the use of said DNA sequences in the production of Ig-type molecules and said ligands or receptors, and the use of said ligands or receptors in therapy, diagnosis and catalysis.Type: GrantFiled: June 6, 1995Date of Patent: June 19, 2001Assignee: Medical Research CouncilInventors: Gregory Paul Winter, Elizabeth Sally Ward, Detlef Güssow
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Patent number: 6225447Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a fist and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.Type: GrantFiled: June 17, 1998Date of Patent: May 1, 2001Assignees: Cambridge Antibody Technology Ltd., Medical Research CouncilInventors: Gregory Paul Winter, Kevin Stuart Johnson, Andrew David Griffiths, Andrew John Hammond Smith
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Patent number: 6184012Abstract: The present invention relates to a method of isolating an enzyme having a desired chemical or biological activity, the method comprising linking the enzyme to a substrate for the enzyme, reacting the enzyme and substrate such that a product is produced which remains linked to the enzyme and isolating the enzyme by selectively isolating the product linked to the enzyme.Type: GrantFiled: April 22, 1999Date of Patent: February 6, 2001Assignee: Medical Research CouncilInventors: Dario Neri, Salvatore Demartis, Adrian Huber, Francesca Viti, Dan S Tawfik, Gregory Paul Winter
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Patent number: 6172197Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.Type: GrantFiled: June 7, 1995Date of Patent: January 9, 2001Assignees: Medical Research Council, Cambridge Antibody Technology LimitedInventors: John McCafferty, Anthony Richard Pope, Kevin Stuart Johnson, Henricus Renerus Jacobus Mattheus Hoogenboom, Andrew David Griffiths, Ronald Henry Jackson, Kaspar Philipp Holliger, James David Marks, Timothy Piers Clackson, David John Chiswell, Gregory Paul Winter, Timothy Peter Bonnert
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Patent number: 6140471Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.Type: GrantFiled: March 30, 1998Date of Patent: October 31, 2000Assignees: Cambridge Antibody Technology, Ltd., Medical Research CouncilInventors: Kevin Stuart Johnson, Gregory Paul Winter, Andrew David Griffiths, Andrew John Hammond Smith, Peter Michael Waterhouse
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Patent number: 6117976Abstract: Molecules comprise (i) a polypeptide (such as calmodulin) which has calcium-dependent binding affinity for ligand and (ii) another polypeptide, the polypeptides preferably being joined by a peptide bond and produced by recombinant expression from a gene fusion. The molecules are useful in detection, immobilization, targeting and purification, cell-labelling, and band-shift assays for determining binding of a member of a specific binding pair (sbp) for complementary sbp member. For purposes of band-shift assays, polypeptide (i) need not have calcium-dependent binding affinity for a ligand, but should have a dissociation constant for a ligand of 10 nM or less, measured at a pH of between 6 and 9 at 20.degree. C. In an alternative embodiment, a calmodulin-binding polypeptide which is, or is derived from, mastoparan is joined, as polypeptide (i) instead of a binding polypeptide, to the other polypeptide.Type: GrantFiled: May 2, 1996Date of Patent: September 12, 2000Assignee: Medical Research CouncilInventors: Dario Neri, Gregory Paul Winter, Claudia De Lalla
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Patent number: 6017732Abstract: Repertoires of first specific binding pair (sbp) members wherein each first sbp member has a chemical moiety bound covalently at an amino acid residue within the binding site are made and may be displayed at the surface of an organism such as a bacteriophage. Methods of making such repertoires may involve the provision of a population of encoding nucleic acid molecules wherein a codon encoding a selectively or preferentially modifiable amino acid is introduced in the region encoding the binding site, for instance by mutation or gene construction. First sbp member (e.g. antibodies) wherein binding to second sbp member (e.g. antigen) is enhanced in or dependent on the presence of the chemical moiety in the binding site may be selected from the repertoires.Type: GrantFiled: September 4, 1997Date of Patent: January 25, 2000Assignee: Medical Research CouncilInventors: Laurent Stephane Anne Therese Jespers, Gregory Paul Winter, Timothy Peter Bonnert, Thomas Martin Simon
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Patent number: 6010884Abstract: DNA constructs comprise a first exon sequence of nucleotides encoding a first peptide or polypeptide, a second exon sequence of nucleotides encoding a second peptide or polypeptide and a third sequence of nucleotides between the first and second sequences encoding a heterologous intron, for example that of Tetrahymena thermophila nuclear pre-rRNA, between RNA splice sites and a site-specific recombination sequence, such as loxP, within the intron, the exons together encoding a product peptide or polypeptide. Such constructs are of use in methods of production of peptides or polypeptides, transcription leading to splicing out of the intron enabling translation of a single chain product peptide or polypeptide. Isolated nucleic acid constructs consisting essentially of a sequence of nucleotides encoding a self-splicing intron with a site-specific recombination sequence within the intron, for use in creation of constructs for expression of peptides or polypeptides, are also provided.Type: GrantFiled: May 29, 1996Date of Patent: January 4, 2000Assignee: Medical Research CouncilInventors: Andrew David Griffiths, Kaspar Philipp Holliger, Ahuva Nissim, Igor Fisch, Gregory Paul Winter
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Patent number: 5969108Abstract: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA.Type: GrantFiled: January 8, 1993Date of Patent: October 19, 1999Assignees: Medical Research Council, Cambridge Antibody Technology LimitedInventors: John McCafferty, Anthony Richard Pope, Kevin Stuart Johnson, Henricus Renerus Jacobus Mattheus Hoogenboom, Andrew David Griffiths, Ronald Henry Jackson, Kaspar Philipp Holliger, James David Marks, Timothy Piers Clackson, David John Chiswell, Gregory Paul Winter, Timothy Peter Bonnert
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Patent number: 5965456Abstract: Disclosed is apparatus for detecting the presence of an analyte of interest in a sample, comprising an immobilised binding partner comprising a solid support and a reversibly binding receptor which is capable of binding to the analyte of interest thereby causing a measurable change in a property of the immobilised partner, and detection means for detecting the measurable change and a method for detecting the presence of an analyte of interest.Type: GrantFiled: April 29, 1997Date of Patent: October 12, 1999Assignee: Biacore ABInventors: Magnus Malmqvist, Gregory Paul Winter
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Patent number: 5965405Abstract: Methods for preparing Fv fragments which lack linking polypeptides in eukaryotic cells are provided.Type: GrantFiled: December 13, 1993Date of Patent: October 12, 1999Assignee: Celltech LimitedInventors: Gregory Paul Winter, Lutz Riechmann, Geoffrey Thomas Yarranton, Mark William Bodmer, Raymond John Owens
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Patent number: 5962255Abstract: Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of a sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.Type: GrantFiled: December 5, 1994Date of Patent: October 5, 1999Assignees: Cambridge Antibody Technology Limited, Medical Research CouncilInventors: Andrew David Griffiths, Samuel Cameron Williams, Peter Michael Waterhouse, Ahuva Nissim, Gregory Paul Winter, Kevin Stuart Johnson, Andrew John Hammond Smith
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Patent number: 5885793Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self-antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from the said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from a rearranged V-gene sequences of unimmunised mammal.Type: GrantFiled: October 26, 1994Date of Patent: March 23, 1999Assignees: Medical Research Council, Cambridge Antibody Technology LimitedInventors: Andrew David Griffiths, Hendricus Renerus Jacobus Mattheus Hoogenboom, James David Marks, John McCafferty, Gregory Paul Winter, Geoffrey Walter Grigg
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Patent number: 5871907Abstract: The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.Type: GrantFiled: March 31, 1994Date of Patent: February 16, 1999Assignees: Medical Research Council, Cambridge Antibody Technology LimitedInventors: Gregory Paul Winter, Kevin Stuart Johnson, Andrew David Griffiths, Andrew John Hammond Smith
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Patent number: 5858657Abstract: The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.Type: GrantFiled: June 7, 1995Date of Patent: January 12, 1999Assignees: Medical Research Council, Cambridge Antibody Technology LimitedInventors: Gregory Paul Winter, Kevin Stuart Johnson, Andrew David Griffiths, Andrew John Hammond Smith