Patents by Inventor Gregory W. Sitton

Gregory W. Sitton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10634672
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target cell-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target cell-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target microorganism are also provided.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: April 28, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Neil Percy, Gregory W. Sitton
  • Patent number: 10619189
    Abstract: A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes a plurality of zirconium oxide particles, a non-ionic surfactant, and an organic iron-chelating reagent. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium, wherein the first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Optionally, the composition includes polyvinylpyrrolidone. Optionally, the composition comprises water. The composition has a pH of about 8.45 to 8.85. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: April 14, 2020
    Assignee: 3M Innovative Properties Company
    Inventors: Gregory W. Sitton, Wensheng Xia, Tonya D. Bonilla
  • Patent number: 10604787
    Abstract: A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium. The first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: March 31, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Gregory W. Sitton, Neil Percy
  • Patent number: 10363557
    Abstract: An apparatus for processing a biological sample is provided. The apparatus has a body having a processing chamber, a first reservoir, a second reservoir, a filter, a first one-way valve, a second one-way valve, a third one-way valve, a first fluid pathway, a second fluid pathway, and a third fluid pathway.
    Type: Grant
    Filed: September 7, 2018
    Date of Patent: July 30, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Jon A. Kirschhoffer, Gregory W. Sitton, Andrew H. Tilstra
  • Patent number: 10281466
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target analyte-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target analyte-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target analyte-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target analyte are also provided.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: May 7, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Neil Percy, Gregory W. Sitton
  • Publication number: 20190112637
    Abstract: The present disclosure provides a nucleic acid amplification method. The method comprises forming an enrichment culture by contacting a sample with a nutrient medium having a formulation that does not include a phosphate buffer component; holding the enrichment culture for a period of time at a temperature that facilitates growth of a target microorganism; after holding the enrichment culture, forming an aqueous composition by mixing a first volume of the enrichment culture with a second volume of a lysis buffer; contacting the aqueous composition with an effective amount of a water-insoluble material that sequesters a substance that interferes with a polymerase-mediated nucleic acid amplification reaction; subjecting the aqueous composition to a thermal lysis process; and, after subjecting the aqueous composition to the thermal lysis process, subjecting a portion of the aqueous composition to a nucleic acid amplification process. A composition for a lysis buffer is also disclosed.
    Type: Application
    Filed: March 24, 2017
    Publication date: April 18, 2019
    Inventors: GREGORY W. SITTON, NEIL PERCY
  • Publication number: 20190001321
    Abstract: An apparatus for processing a biological sample is provided. The apparatus comprises a body having a processing chamber, a first reservoir, a second reservoir, a filter, a first one-way valve, a second one-way valve, a first fluid pathway, and a second fluid pathway. The first fluid pathway and the second fluid pathway pass through the filter. A method of processing a sample using the apparatus is also provided. The method comprises placing a sample comprising a liquid into the processing chamber; urging at least a portion of the liquid through the filter; after urging the at least a portion of the liquid through the filter, urging a portion of a back-flush liquid through the filter into the process chamber to form a first processed sample; and analyzing at least a portion of the first processed sample to detect an indication of a microorganism.
    Type: Application
    Filed: September 7, 2018
    Publication date: January 3, 2019
    Inventors: JON A. KIRSCHHOFFER, GREGORY W. SITTON, ANDREW H. TILSTRA
  • Patent number: 10099217
    Abstract: An apparatus for processing a biological sample is provided. The apparatus includes a body having a processing chamber, a first reservoir, a second reservoir, a filter, a first one-way valve, a second one-way valve, a first fluid pathway, and a second fluid pathway. The first fluid pathway and the second fluid pathway pass through the filter. A method of processing a sample using the apparatus is also provided. The method includes placing a sample comprising a liquid into the processing chamber; urging at least a portion of the liquid through the filter; after urging the at least a portion of the liquid through the filter, urging a portion of a back-flush liquid through the filter into the process chamber to form a first processed sample; and analyzing at least a portion of the first processed sample to detect an indication of a microorganism.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: October 16, 2018
    Assignee: 3M Innovative Properties Company
    Inventors: Jon A. Kirschhoffer, Gregory W. Sitton, Andrew H. Tilstra
  • Publication number: 20180258458
    Abstract: The present disclosure provides a method of detecting a target bacterium. The method includes forming a primary enrichment culture that includes a test sample, a primary enrichment medium and a redox dye; incubating the primary enrichment culture for a first period of time equal to eight hours or less; assessing the primary enrichment culture during the first period of time to detect whether the redox dye changes from a first state to a second state; if the second state is detected in the primary enrichment culture during the first period of time, mixing a portion of the primary enrichment culture with a secondary enrichment medium to form a secondary enrichment culture and incubating the secondary enrichment culture for a second period of time; and performing a test to detect the target bacterium using a portion of the second enrichment culture.
    Type: Application
    Filed: August 29, 2016
    Publication date: September 13, 2018
    Inventor: GREGORY W. SITTON
  • Publication number: 20180142280
    Abstract: A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes a plurality of zirconium oxide particles, a non-ionic surfactant, and an organic iron-chelating reagent. The organic iron-chelating reagent has a first affmity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium, wherein the first affmity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Optionally, the composition includes polyvinylpyrrolidone. Optionally, the composition comprises water. The composition has a pH of about 8.45 to 8.85. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
    Type: Application
    Filed: May 9, 2016
    Publication date: May 24, 2018
    Inventors: GREGORY W. SITTON, WENSHENG XIZ, TONYA D. BONILLA
  • Publication number: 20170369929
    Abstract: A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium. The first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
    Type: Application
    Filed: December 21, 2015
    Publication date: December 28, 2017
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: GREGORY W. SITTON, NEIL PERCY
  • Patent number: 9845498
    Abstract: A method of detecting an analyte is provided. The method includes providing a sample, a container 110 with a wall 115, and a catalyst for a luminescent reaction. The wall includes a colored portion 115b. The method further comprises forming a reaction in the container and detecting the presence or absence of light emitted from the reaction mixture in the container. Detecting light emitted from the container can comprise detecting light passing through the colored portion. The colored portion can be detected visually and the color can be associated with the identity of an analyte-specific reagent disposed in the container. Kits comprising the container and a catalyst for a luminescent reaction are also provided.
    Type: Grant
    Filed: September 16, 2014
    Date of Patent: December 19, 2017
    Assignee: 3M Innovative Properties Company
    Inventors: Neil Percy, Gregory W. Sitton
  • Publication number: 20170299586
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target analyte-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target analyte-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target analyte-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target analyte are also provided.
    Type: Application
    Filed: October 5, 2015
    Publication date: October 19, 2017
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: NEIL PERCY, GREGORY W. SITTON
  • Publication number: 20170219577
    Abstract: A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target cell-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target cell-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target microorganism are also provided.
    Type: Application
    Filed: October 5, 2015
    Publication date: August 3, 2017
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: NEIL PERCY, GREGORY W. SITTON
  • Patent number: 9677981
    Abstract: The present disclosure provides an assembly for preparing a sample for analysis. The assembly comprises a hollow body comprising first opening, an outlet with a second opening, and a fluid pathway extending therebetween; a filter element operatively interposed in the fluid pathway; and a closure comprising a third opening and a chamber, the closure being slideably engaged with the outlet. The chamber and the outlet are dimensioned so that the outlet is sealingly engaged with the chamber. A method of using the assembly to detect a microorganism is also provided.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 13, 2017
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Gregory W. Sitton, Andrew H. Tilstra, Wensheng Xia, Jon A. Kirschhoffer
  • Publication number: 20160296927
    Abstract: An apparatus for processing a biological sample is provided. The apparatus comprises a body having a processing chamber, a first reservoir, a second reservoir, a filter, a first one-way valve, a second one-way valve, a first fluid pathway, and a second fluid pathway. The first fluid pathway and the second fluid pathway pass through the filter. A method of processing a sample using the apparatus is also provided. The method comprises placing a sample comprising a liquid into the processing chamber; urging at least a portion of the liquid through the filter; after urging the at least a portion of the liquid through the filter, urging a portion of a back-flush liquid through the filter into the process chamber to form a first processed sample; and analyzing at least a portion of the first processed sample to detect an indication of a microorganism.
    Type: Application
    Filed: December 8, 2014
    Publication date: October 13, 2016
    Inventors: JON A. KIRSCHHOFFER, GREGORY W. SITTON, ANDREW H. TILSTRA
  • Publication number: 20160025607
    Abstract: The present disclosure provides an assembly for preparing a sample for analysis. The assembly comprises a hollow body comprising first opening, an outlet with a second opening, and a fluid pathway extending therebetween; a filter element operatively interposed in the fluid pathway; and a closure comprising a third opening and a chamber, the closure being slideably engaged with the outlet. The chamber and the outlet are dimensioned so that the outlet is sealingly engaged with the chamber. A method of using the assembly to detect a microorganism is also provided.
    Type: Application
    Filed: March 13, 2014
    Publication date: January 28, 2016
    Inventors: GREGORY W. SITTON, ANDREW H. TILSTRA, WENSHENG XIA, JON A. KIRSCHHOFFER
  • Patent number: 9038503
    Abstract: Tools (100) are provided for use in uncapping one or more of a plurality of linearly-oriented, spaced-apart tubes; each cap having a cap upper surface with a depression. The tools comprise a body (30) having first portion (40) for engagement by a user and a second portion (50) for engaging a plurality of caps. The second portion comprises a plurality of spaced-apart projections (60). Each projection is configured for releasably engaging a cap. The projection may comprise two or more spaced-apart projection elements (62,64). Optionally, the first portion is configured in a non-coplanar relationship with respect to the second portion. Methods of use are also provided.
    Type: Grant
    Filed: August 2, 2012
    Date of Patent: May 26, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Cheryl A. Bartholomew, Jason W. Bjork, Neil Percy, Gregory W. Sitton
  • Publication number: 20150004681
    Abstract: A method of detecting an analyte is provided. The method includes providing a sample, a container 110 with a wall 115, and a catalyst for a luminescent reaction. The wall includes a colored portion 115b. The method further comprises forming a reaction in the container and detecting the presence or absence of light emitted from the reaction mixture in the container. Detecting light emitted from the container can comprise detecting light passing through the colored portion. The colored portion can be detected visually and the color can be associated with the identity of an analyte-specific reagent disposed in the container. Kits comprising the container and a catalyst for a luminescent reaction are also provided.
    Type: Application
    Filed: September 16, 2014
    Publication date: January 1, 2015
    Inventors: NEIL PERCY, GREGORY W. SITTON
  • Patent number: 8852894
    Abstract: A method of detecting an analyte is provided. The method includes providing a sample, a container 110 with a wall 115, and a catalyst for a luminescent reaction. The wall includes a colored portion 115b. The method further comprises forming a reaction in the container and detecting the presence or absence of light emitted from the reaction mixture in the container. Detecting light emitted from the container can comprise detecting light passing through the colored portion. The colored portion can be detected visually and the color can be associated with the identity of an analyte—specific reagent disposed in the container. Kits comprising the container and a catalyst for a luminescent reaction are also provided.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: October 7, 2014
    Assignee: 3M Innovative Properties Company
    Inventors: Neil Percy, Gregory W. Sitton