Abstract: Provided are a modified mesenchymal stem cell and culture supernatant thereof, and a pharmaceutical composition comprising said cell or culture supernatant thereof. The mesenchymal stem cell is capable of expressing: (1) a first protein, which is selected from FGF21 or a variant thereof, or a first fusion protein comprising the FGF21 or the variant thereof and (2) a second protein, which is selected from GLP-1 or a variant thereof or a second fusion protein comprising the GLP-1 or the variant thereof. Also provided is the use of the modified mesenchymal stem cell and culture supernatant thereof, and the pharmaceutical composition comprising said cell or culture supernatant thereof in the treatment of metabolic diseases and in the preparation of a medicament for treating metabolic diseases.

Abstract: A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and/or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Abstract: Provided are a modified mesenchymal stem cell and culture supernatant thereof, and a pharmaceutical composition comprising said cell or culture supernatant thereof. The mesenchymal stem cell is capable of expressing: (1) a first protein, which is selected from FGF21 or a variant thereof, or a first fusion protein comprising the FGF21 or the variant thereof and (2) a second protein, which is selected from GLP-1 or a variant thereof or a second fusion protein comprising the GLP-1 or the variant thereof. Also provided is the use of the modified mesenchymal stem cell and culture supernatant thereof, and the pharmaceutical composition comprising said cell or culture supernatant thereof in the treatment of metabolic diseases and in the preparation of a medicament for treating metabolic diseases.

Abstract: It discloses a fibroblast growth factor 21 variant, a fusion protein comprising such fibroblast growth factor 21 variant, a GLP-1 variant and a FC sequence, and a use thereof. The fusion protein of the present invention has high activity, long half-life and a novel structure, and can significantly decrease blood sugar, body weight, and improve fat metabolism. The present invention also provides a fusion gene, an expression construct, and a host cell comprising an encoding nucleotide sequence of the fusion protein, and a use of the fusion protein, the fusion gene, the expression construct, the host cell, and the pharmaceutical composition in the preparation of drugs for treating obesity, hyperlipidemia, diabetes, and cardiovascular and cerebrovascular diseases.

Abstract: The present invention provides a sphingosine kinase 1, a fusion protein comprising the same, and a use thereof. The sphingosine kinase 1 and the fusion protein comprising the same have significant effects in lowering blood sugar and body weight, and are useful for the preparations of protein drugs for controlling metabolic diseases such as obesity and diabetes. It also provides a protein drug, which is a fusion protein containing a sphingosine kinase 1 or an amino acid sequence having the activity thereof. The fusion protein comprises a sphingosine kinase 1 (SPHK1) or an amino acid sequence having the activity thereof, a FC sequence and a linker sequence. The protein drug can significantly decrease blood sugar, blood fat and body weight, and improve fat metabolism.

Abstract: Provided are a modified mesenchymal stem cell and culture supernatant thereof, and a pharmaceutical composition comprising said cell or culture supernatant thereof. The mesenchymal stem cell is capable of expressing: (1) a first protein, which is selected from FGF21 or a variant thereof, or a first fusion protein comprising the FGF21 or the variant thereof and (2) a second protein, which is selected from GLP-1 or a variant thereof or a second fusion protein comprising the GLP-1 or the variant thereof. Also provided is the use of the modified mesenchymal stem cell and culture supernatant thereof, and the pharmaceutical composition comprising said cell or culture supernatant thereof in the treatment of metabolic diseases and in the preparation of a medicament for treating metabolic diseases.

Abstract: A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and/or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Abstract: A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and/or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Abstract: A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and/or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Abstract: A method for sequencing a biological molecule, such as a nucleic acid molecule, and a method for detecting and/or correcting sequencing error(s) in the sequencing results are provided. Kits and systems based on the above methods are also provided.

Abstract: A method and a device for controlling service transmission can implement service forwarding. The method includes: receiving a connection request message, where the connection request message includes user information; determining value-added service system VAS information according to the user information; receiving a first data packet of the service that is sent by a sending end; obtaining, according to the VAS information, a second data packet from the at least one destination VAS; and sending the second data packet to a receiving end. A VAS performs value-added processing on a data packet, and returns the data packet to a gateway device, allowing the gateway device to perform service arrangement flexibly according to dynamic information about a user.

Abstract: A method, an apparatus, and a system for accessing service by a Mobile Station (MS) are provided by the present disclosure. The method comprises the following steps: Gateway General Packet Radio Service (GPRS) Support Node (GGSN) or a next level gateway of the GGSN receives a service request carrying Virtual Access Point Name (VAPN) or a service request not including APN transmitted from a MS, performs a Deep Packet Inspection (DPI) processing to the service request, and identifies the type of the service; GGSN or the gateway sends the service request to the external data network corresponding to the service type. Therefore, accessing various types of mobile data services can be achieved through the service identification function of GGSN or GGSN gateway without modifying MS APN configuration by the mobile MS, therefore the service experiences for a MS user is enhanced effectively.

Abstract: A method and an apparatus for charging, related to the technical field of communications, are invented in an attempt to enhance charging accuracy of a charging system. A method for charging comprises: acquiring an IMEI number of a target charging terminal; determining a type of the target charging terminal by utilizing the IMEI number of the target charging terminal, according to a corresponding relationship between relevant information of a terminal identifier and a terminal type; and charging the target charging terminal according to the type of the target charging terminal.

Abstract: A method of intercutting advertisement disclosed comprises: receiving a playing request message carrying user information and media information from a user terminal; determining whether advertisement needs to be intercut for the user based on the user information and the media information; when it is determined that advertisement needs to be intercut for the user, making a pause in playing a stream media corresponding to the playing request message, obtaining an advertisement stream corresponding to the user information and the media information, and delivering the advertisement stream to the user to play; when it is detected that play of the advertisement stream is finished, sending the playing request message to a stream media server, and forwarding to the user terminal the stream media obtained from the stream media server, corresponding to the playing request message.

Abstract: A method and a device for controlling service transmission can implement service forwarding. The method includes: receiving a connection request message, where the connection request message includes user information; determining value-added service system VAS information according to the user information; receiving a first data packet of the service that is sent by a sending end; obtaining, according to the VAS information, a second data packet from the at least one destination VAS; and sending the second data packet to a receiving end. A VAS performs value-added processing on a data packet, and returns the data packet to a gateway device, allowing the gateway device to perform service arrangement flexibly according to dynamic information about a user.

Abstract: A method and an apparatus for charging, related to the technical field of communications, are invented in an attempt to enhance charging accuracy of a charging system. A method for charging comprises: acquiring an IMEI number of a target charging terminal; determining a type of the target charging terminal by utilizing the IMEI number of the target charging terminal, according to a corresponding relationship between relevant information of a terminal identifier and a terminal type; and charging the target charging terminal according to the type of the target charging terminal.

Abstract: A service charging method, including: receiving a service packet, performing a protocol resolution on the service packet and obtaining charging information when determining that the protocol resolution needs to be performed on the service packet; and performing a service charging according to the charging information and a preset charging policy. The invention further provides a service charging system, a network access server and a protocol resolving device. Thus, it is easier and more convenient for a user to use a service, and no complex traffic back-patching solution needs to be implemented.

Abstract: In general, the invention features methods and systems for sequencing of nucleic acids based on the measurement of the incorporation of fluorogenic nucleotides in microreactors. The invention provides numerous advantages over previous systems such as unambiguous determination of sequence, fast cycle time, long read lengths, low overall cost of reagents, low instrument cost, and high throughput. The invention also features methods and kits for nucleic acid amplification. The amplification and sequencing aspects of the invention may or may not be employed in conjunction with one another. The invention also features fluorogenic nucleotides that may be used in the sequencing methods of the invention.

Abstract: An advice of charging (AoC) method, system, and mobile terminal thereof are provided. The method includes receiving AoC information sent by a service charging server, and then displaying the AoC information. The mobile terminal includes a receiving module adapted to receive AoC information sent by a service charging server, and a display module adapted to display the received AoC information. The system includes a terminal, a service charging server, a service gateway, and a charging engine. The present invention is applicable to pre-consumption prompt, post-consumption notification, and prompts for data services such as browsing, streaming media, and mail.

Abstract: A message processing method, device, and system are provided in the embodiments of the present invention. The method comprises: receiving service messages and identifying the protocol type of the service messages; performing protocol adaptation according to the protocol type; and reconstructing the content of the service messages after protocol adaptation according to the corresponding content reconstructing policies and sending the service messages after content reconstructing. The present invention implements information embedment into the content over different protocols without proxy server settings.