Abstract: A roller bottle handling system includes a culture rack storage station for storing a plurality of culture racks each for accommodating a plurality of roller bottles each filled with a culture medium and cells, and a mass-handling facility for extracting completed cultures from roller bottles and replacing a culture medium in the roller bottles with a new culture medium. The roller bottle handling system further includes an unloading station for taking out roller bottles from culture racks delivered from the culture rack storage station and delivering the roller bottles to the mass-handling facility, and a loading station for charging roller bottles filled with a culture medium and cells into culture racks. Automatic guided vehicles or overhead traveling cranes are movable to deliver culture racks, one at a time, between the culture rack storage station and the unloading and loading stations.