Patents by Inventor Hakho Lee

Hakho Lee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20160334398
    Abstract: Disclosed herein are compositions and methods for exosome detection with high sensitivity by using a nano-plasmonic sensor. The nano-plasmonic sensor comprises a plurality of nanoapertures suitable for transmission measurements. The detection sensitivity is on the order of 104-fold higher than western blotting and 102-fold higher than enzyme-linked immunosorbent assay (ELISA). A portable imaging system is also disclosed, enabling rapid and high-throughput detection of exosomes. The nano-plasmonic sensor and imaging system can be useful in diagnostics.
    Type: Application
    Filed: December 2, 2014
    Publication date: November 17, 2016
    Inventors: Ralph WEISSLEDER, Hakho LEE, Hyungsoon IM, Cesar CASTRO
  • Publication number: 20160313332
    Abstract: In one aspect, a system for isolating particles includes a first array of magnets, a second array of magnets arranged generally in parallel with and spaced apart from the first array of magnets, and a micro fluidic device. The micro fluidic device includes a substrate, an inlet arranged on the substrate and configured to receive a fluid sample, an outlet arranged on the substrate, a first region of the substrate including a channel connected to the inlet, where the first region of the substrate is arranged to sandwich the channel between the first and second arrays of magnets.
    Type: Application
    Filed: December 17, 2014
    Publication date: October 27, 2016
    Inventors: Hakho Lee, Ralph Weissleder, Kyungheon Lee, Jun Song
  • Publication number: 20160202163
    Abstract: The disclosure features systems and methods for measuring and diagnosing target constituents bound to labeling particles in a sample. The systems include a radiation source, a sample holder, a detector configured to obtain one or more diffraction patterns of the sample each including information corresponding to optical properties of sample constituents, and an electronic processor configured to, for each of the one or more diffraction patterns: (a) analyze the diffraction pattern to obtain amplitude information and phase information corresponding to the sample constituents; (b) identify one or more particle-bound target sample constituents based on at least one of the amplitude information and the phase information; and (c) determine an amount of at least one of the particle-bound target sample constituents in the sample based on at least one of the amplitude information and the phase information.
    Type: Application
    Filed: August 18, 2014
    Publication date: July 14, 2016
    Inventors: Ralph Weissleder, Hakho Lee, Hyungsoon Im, Cesar Castro
  • Publication number: 20150258222
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Application
    Filed: January 9, 2015
    Publication date: September 17, 2015
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Publication number: 20150125884
    Abstract: The present invention provides novel methods of magnetically labeling a bacterial cell by contacting the call with an affinity ligand and subsequently contacting the cell with a magnetic agent, where the affinity ligand and magnetic agent include bioorthogonally reactive groups that can react with each other to form a covalent bond. Compounds, compositions, kits and applications of the method are also described.
    Type: Application
    Filed: March 8, 2013
    Publication date: May 7, 2015
    Inventors: Ghyslain Budin, Ralph Weissleder, Hakho Lee, Hyun Jung Chung
  • Patent number: 8945628
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Grant
    Filed: May 26, 2011
    Date of Patent: February 3, 2015
    Assignee: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Publication number: 20140295460
    Abstract: Determining a presence of a target analyte in a fluid sample includes mixing multiple magnetic particles with the fluid sample, in which the magnetic particles are each bound to one or more binding moieties that specifically bind to the target analyte, flowing the fluid sample containing the magnetic particles through a fluidic channel, exposing the fluid sample in the fluidic channel to a magnetic field, measuring a signal from a Hall effect sensor while the fluid sample flows through the fluidic channel, and determining whether the target analyte is present in the fluid sample when the measured signal is in a first range of values.
    Type: Application
    Filed: November 21, 2012
    Publication date: October 2, 2014
    Applicant: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Publication number: 20140178901
    Abstract: This invention relates to methods for diagnosing cancer, e.g., cancer of epithelial origin, by detecting the presence of tumor cells in a sample, based (at least in some embodiments) on the quantification of levels of four biomarkers, MUC1, EGFR, EpCAM, and HER2. In some embodiments, the methods are performed using diagnostic magnetic resonance (DMR), e.g., with a portable relaxometer or MR imager.
    Type: Application
    Filed: March 21, 2012
    Publication date: June 26, 2014
    Applicant: The General Hospital Corporation
    Inventors: Ralph Weissleder, Hakho Lee, Cesar Castro
  • Publication number: 20140011217
    Abstract: A portable magnetic resonance system includes a permanent magnet, a nuclear magnetic resonance probe, and control electronics. The control electronics are configured to transmit to the probe a magnetic resonance excitation signal having an excitation frequency f, receive from the probe a magnetic resonance measurement signal, detect in the magnetic resonance measurement signal a magnetic resonance frequency f0, and automatically adjust the excitation frequency f until the difference between the excitation frequency and the magnetic resonance frequency is approximately equal to a target offset.
    Type: Application
    Filed: March 21, 2012
    Publication date: January 9, 2014
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Publication number: 20130271250
    Abstract: A magnetic device includes a substrate and an array including a plurality of magnetic particles, in which a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles in the array alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle or directly adjacent magnetic particles in the array.
    Type: Application
    Filed: September 27, 2011
    Publication date: October 17, 2013
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Ralph Weissleder, Hakho Lee, David Issadore
  • Publication number: 20130195767
    Abstract: A magnetic nanoparticle includes a magnetic core and a superparamagnetic outer shell, in which the outer shell enhances magnetic properties of the nanoparticle. The enhanced magnetic properties of the magnetic nanoparticle allow for highly sensitive detection as well as diminished non-specific aggregation of nanoparticles.
    Type: Application
    Filed: May 26, 2011
    Publication date: August 1, 2013
    Inventors: Ralph Weissleder, Hakho Lee, Tae-Jong Yoon
  • Publication number: 20110091987
    Abstract: The present application describes devices, systems, and techniques related to a chip-based, miniaturized NMR diagnostic platform for rapid, quantitative and multi-channeled detection of biological targets.
    Type: Application
    Filed: October 6, 2008
    Publication date: April 21, 2011
    Inventors: Ralph Weissleder, Hakho Lee, Donhee Ham, Nan Sun, Yong Liu
  • Publication number: 20100006439
    Abstract: Methods and apparatus for manipulation, detection, imaging, characterization, sorting and/or assembly of biological or other materials, involving an integration of CMOS or other semiconductor-based technology and microfluidics. In one implementation, various components relating to the generation of electric and/or magnetic fields are implemented on an IC chip that is fabricated using standard protocols. The generated electric and/or magnetic fields are used to manipulate and/or detect one or more dielectric and/or magnetic particles and distinguish different types of particles. A microfluidic system is fabricated either directly on top of the IC chip, or as a separate entity that is then appropriately bonded to the IC chip, to facilitate the introduction and removal of cells in a biocompatible environment, or other particles/objects of interest suspended in a fluid.
    Type: Application
    Filed: September 15, 2009
    Publication date: January 14, 2010
    Applicant: President and Fellows of Harvard College
    Inventors: Donhee Ham, Robert Westervelt, Thomas Hunt, Yong Liu, Hakho Lee
  • Publication number: 20090168162
    Abstract: Microscopy methods and apparatus for manipulation, detection, imaging, characterization, sorting and/or assembly of biological or other materials, involving an integration of CMOS or other semiconductor-based technology and microfluidics in connection with a microscope. In one implementation, a microscope including optics and a stage is outfitted with various components relating to the generation of electric and/or magnetic fields, which are implemented on an IC chip. A microfluidic system is fabricated either directly on top of the IC chip, or as a separate entity that is then appropriately bonded to the IC chip, to facilitate the introduction and removal of cells in a biocompatible environment, or other particles/objects of interest suspended in a fluid. The patterned electric and/or magnetic fields generated by the IC chip can trap and move biological cells or other objects inside the microfluidic system to facilitate viewing via the microscope.
    Type: Application
    Filed: January 12, 2007
    Publication date: July 2, 2009
    Applicant: President and Fellows of Harvard College
    Inventors: Donhee Ham, Robert Westervelt, Thomas Hunt, Yong Liu, Hakho Lee
  • Publication number: 20060020371
    Abstract: Methods and apparatus for manipulation, detection, imaging, characterization, sorting and/or assembly of biological or other materials, involving an integration of CMOS or other semiconductor-based technology and microfluidics. In one implementation, various components relating to the generation of electric and/or magnetic fields are implemented on an IC chip that is fabricated using standard protocols. The generated electric and/or magnetic fields are used to manipulate and/or detect one or more dielectric and/or magnetic particles and distinguish different types of particles. A microfluidic system is fabricated either directly on top of the IC chip, or as a separate entity that is then appropriately bonded to the IC chip, to facilitate the introduction and removal of cells in a biocompatible environment, or other particles/objects of interest suspended in a fluid.
    Type: Application
    Filed: April 13, 2005
    Publication date: January 26, 2006
    Applicant: President and Fellows of Harvard College
    Inventors: Donhee Ham, Robert Westervelt, Thomas Hunt, Yong Liu, Hakho Lee
  • Publication number: 20040262210
    Abstract: A micro-electromagnet matrix captures and controls the movement of particles with nanoscale resolution. The micro-electromagnet matrix includes multiple layers of microconductors, each layer of microconductors being orthogonal to an adjacent layer or microconductors. The layers of microconductors are formed on a substrate and have insulating layers therebetween. The field patterns produced by the micro-electromagnet matrix enable precise manipulation of particles. The micro-electro-magnet matrix produces single or multiple independent field peaks in the magnetic field that are used to trap, move, or rotate the particles. The micro-electromagnet matrix also produces electromagnetic fields to probe and detect particles.
    Type: Application
    Filed: May 3, 2004
    Publication date: December 30, 2004
    Inventors: Robert M. Westervelt, Chungsok Lee, Hakho Lee