Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 ?m.

Abstract: The invention relates to methods of reducing acrylamide during high temperature cooking of carbohydrate-containing foods, in particular vegetables or tubers which are deep fried. In particular, the invention relates to a method of reducing acrylamide during cooking of a carbohydrate-containing food, said method comprising at least the following steps: contacting said food with a first hydroxy acid selected from lactic, malic and tartaric acids at a pH less than or equal to its pKa, or its lowest pKa; part-cooking said food at a temperature at which the Maillard reaction occurs whereby to form a part-cooked food;optionally packaging and/or storing said part-cooked food; contacting said part-cooked food with a second ?-hydroxy acid selected from lactic, malic and tartaric acids at a pH less than or equal to its pKa, or its lowest pKa; and subsequently further cooking said food at a temperature at which the Maillard reaction occurs.

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

Abstract: A method of continuous separation of a plasmid from a process feed in an apparatus with at least three chromatography columns packed with separation matrix particles, wherein while one chromatography column is loaded with the process feed, another chromatography column is eluted with an eluent to recover the separated plasmid, and yet another chromatography column is eluted with a further eluent to remove contaminants.

Abstract: The present invention relates to a method for determining operational status of a chromatography column (1; 39, 47, 59; 107, 109, 111, 113), comprising detecting a feed signal (21; 201) representative of the composition of a feed material provided to the inlet of the column; detecting the UV absorbance in the feed material, detecting an effluent signal (23; 203, 205, 207, 209) representative of the composition of the effluent from the column; and using the feed signal and the effluent signal to determine operational status of the column. The feed signal is generated using a first UV detector having a first UV cell pathlength operating at a first UV wavelength and in the effluent signal is generated using a second UV detector having a second UV cell pathlength operating at a second UV wavelength.

Abstract: The present invention relates to a method for purifying a target product in a flow-through chromatography system comprises at least a first column loaded with feed material from a feed source. The at least first column is purged after binding of impurities and wherein the outlet of purged material from the column is subsequently passed to the feed source.

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 ?m. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

Abstract: The use of acid treatment of uncooked French fries prior to cooking by roasting, frying, grill or baking whereby to reduce the acrylamide content of the cooked product.

Abstract: A device 20 for requesting a vehicle to a location associated with the device. The device 20 comprises input means 126 arranged to generate a vehicle request upon receipt of a user input, said device 20 being arranged to allow at least a second vehicle request to be generated using the input means before the vehicle associated with a first vehicle request has departed from the location associated with the device. The device 20 is arranged, for each vehicle request, to generate a message indicative of the status of the vehicle request and to display the generated message on a display 124. The device is arranged to stop displaying the message on the display 124 when the requested vehicle departs from the location.

Abstract: The use of acid treatment of uncooked French fries prior to cooking by roasting, frying, grill or baking whereby to reduce the acrylamide content of the cooked product.

Abstract: The use of acid treatment of uncooked French fries prior to cooking by roasting, frying, grill or baking whereby to reduce the acrylamide content of the cooked product.

Abstract: The present invention relates to a method of separating nucleic acids from a liquid, which comprises providing a mobile phase comprising nucleic acids and a first salt, which forms lyotropic ions when dissolved; passing said mobile phase across a hydrophobic interaction chromatography (HIC) matrix to adsorb deoxyribonucleic acid(s); passing an eluent across said matrix to desorb one or more deoxyribonucleic acid(s), which eluent comprises the first salt as well as an increasing gradient provided by a second salt, which second salt forms less lyotropic ions when dissolved than the first salt; and isolating at least one fraction comprising separated deoxyribonucleic acid(s).

Abstract: The invention relates to a method for determining luminescent molecules by means of optical excitation in confocal measurement volumes using a diffractive optical element. The method is particularly suitable for single-molecule determination, e.g. by means of fluorescence correlation spectroscopy or by means of dynamic light scattering. An apparatus suitable for carrying out the method according to the invention is furthermore disclosed.

Abstract: The invention relates to the use of acid treatment of uncooked French fries prior to cooking by roasting, frying, grilling or baking whereby to reduce the acrylamide content of the cooked product.

Abstract: The invention relates to a method for determining luminescent molecules by means of optical excitation in confocal measurement volumes using a diffractive optical element. The method is particularly suitable for single-molecule determination, e.g. by means of fluorescence correlation spectroscopy or by means of dynamic light scattering. An apparatus suitable for carrying out the method according to the invention is furthermore disclosed.

Abstract: A connector for electrically and mechanically interconnecting multiple pairs of small coaxial conductor pairs includes a shield bus layer and signal conductor traces on opposite sides of a flexible dielectric substrate. Terminal portions of the signal conductor traces span an opening through the connector giving access to each signal conductor trace for soldering the traces to arrayed terminals on a circuit assembly, and are located accurately, so that mass soldering techniques can be utilized, with the connector located with respect to the circuit assembly by registration pins and holes. Portions of the shield bus are exposed to be soldered to a respective terminal on a circuit assembly, and to allow the shield conductors of the coaxial pairs to be soldered to the shield bus layer. Thermal breaks are defined in the shield bus layer to protect soldered junctions between the signal conductor traces and the signal conductors of coaxial pairs and the terminals of the circuit assembly.

Abstract: A carrier, or transfer frame, includes a pair of support portions located on opposite sides of an opening defined by the frame to receive portions of individual conductors of a cable, with the conductors extending across the opening and held in desired positions relative to each other during transportation prior to installation in an electrical circuit. Each conductor is connected electrically to a respective test contact pad of electrically conductive material located on one of the support portions. The transfer frame is placed and held in a desired position adjacent a circuit while the conductors are soldered, with access to the individual conductors, including shield conductors, being gained through the opening defined by the transfer frame.