Patents by Inventor Hans-Peter Josel

Hans-Peter Josel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8137616
    Abstract: The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
    Type: Grant
    Filed: April 1, 2004
    Date of Patent: March 20, 2012
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Gregor Sagner, Ingrid Bechler, Jochen Bolte, Dieter Heindl, Hans-Peter Josel, Martin Gutekunst, Rudolf Sebl, Christoph Mueller
  • Publication number: 20110287408
    Abstract: A conjugate comprising a dye-labeled microporous crystal, a stop-cock moiety, and covalently bound thereto an affinity binding agent is disclosed. The dye-labeled microporous crystal is a zeolite crystal, such as a zeolite L crystal, having a large number of channels in its interior into which the dye is loaded. The stop-cock moiety can be functionalized with an amino group to which a carboxyester group can be attached. The affinity binding agent allows for the binding to a biological moiety. The conjugate of the moiety can be used in in-vivo and/or in-vitro imaging applications.
    Type: Application
    Filed: May 3, 2011
    Publication date: November 24, 2011
    Inventors: Zoran Popovic, Manuel Matthias Tsotsalas, Michael Busby, Luisa De Cola, Gion Calzaferri, Hans-Peter Josel
  • Patent number: 8058431
    Abstract: Disclosed is a fluorescent dye comprising a benzothiazolium moiety and a pyrimidinium moiety connected by a mono-methine bridge, characterized in that (i) the 2-position of the pyrimidine carries a substituent which starts with a C-atom and (ii) the 5- and 6- positions of the pyrimidine ring are an integral part of a further aromatic ring structure as exemplified by the following:
    Type: Grant
    Filed: April 3, 2009
    Date of Patent: November 15, 2011
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Hans-Peter Josel, Dieter Heindl, Bernhard Irlinger, Christian Weilke
  • Publication number: 20110151550
    Abstract: The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
    Type: Application
    Filed: February 8, 2011
    Publication date: June 23, 2011
    Applicant: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: Gregor Sagner, Ingrid Bechler, Jochen Bolte, Dieter Heindl, Hans-Peter Josel, Martin Gutekunst, Rudolf Sebl, Christoph Mueller
  • Patent number: 7960124
    Abstract: A conjugate comprising a dye-labeled microporous crystal, a stop-cock moiety, and covalently bound thereto an affinity binding agent is disclosed. The dye-labeled microporous crystal is a zeolite crystal, such as a zeolite L crystal, having a large number of channels in its interior into which the dye is loaded. The stop-cock moiety can be functionalized with an amino group to which a carboxyester group can be attached. The affinity binding agent allows for the binding to a biological moiety. The conjugate of the moiety can be used in in-vivo and/or in-vitro imaging applications.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: June 14, 2011
    Assignees: Roche Diagnostics Operations, Inc., Universitaet Bern
    Inventors: Zoran Popovic, Manuel Matthias Tsotsalas, Michael Busby, Luisa De Cola, Gion Calzaferri, Hans-Peter Josel
  • Publication number: 20110092689
    Abstract: Disclosed are pyridinyl-isoquinoline-dione derivatives, methods of producing these derivatives, conjugates comprising the pyridinyl-isoquinoline dione derivatives and (i) a solid support, or (ii) a biomolecule, methods of producing these conjugates as well as the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET).
    Type: Application
    Filed: October 5, 2010
    Publication date: April 21, 2011
    Inventors: Rainer Beckert, Frank Bergmann, Dieter Heindl, Rupert Herrmann, Hans-Peter Josel, Thomas Welzel
  • Patent number: 7897404
    Abstract: The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 1 and 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomolecule-linker product of uniform stoichiometry in a pre-selected amount.
    Type: Grant
    Filed: August 26, 2001
    Date of Patent: March 1, 2011
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Eva Hoess, Herbert Andres, Frederic Donie, Rudolf Vogel, Hans-Peter Josel, Rupert Hermann, Herbert Von Der Eltz
  • Publication number: 20100258742
    Abstract: The present invention provides kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. In more detail, with the method of the present invention it is possible to verify the mixing ratio of a first component comprising a first dye with a second component comprising a second dye.
    Type: Application
    Filed: March 29, 2010
    Publication date: October 14, 2010
    Inventors: Dieter Heindl, Waltraud Ankenbauer, Hans-Peter Josel, Christian Weilke
  • Patent number: 7807402
    Abstract: A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation.
    Type: Grant
    Filed: April 22, 2008
    Date of Patent: October 5, 2010
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Carina Horn, Hans-Peter Josel, Juergen Spinke, Rupert Herrmann, Dieter Heindl
  • Patent number: 7759470
    Abstract: The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.
    Type: Grant
    Filed: February 16, 2007
    Date of Patent: July 20, 2010
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Dieter Heindl, Frank Bergmann, Hans-Peter Josel, Dirk Kessler, Gunter Lampert, Christian Weilke
  • Publication number: 20090275037
    Abstract: The present invention is directed to a fluorescent dye comprising a benzothiazolium moiety and a pyrimidinium moiety connected by a mono-methine bridge, characterized in that (i) the 2-position of the pyrimidine carries a substituent which starts with a C-atom and (ii) the 5- and 6-positions of the pyrimidine ring are an integral part of a further aromatic ring structure.
    Type: Application
    Filed: April 3, 2009
    Publication date: November 5, 2009
    Inventors: Hans-Peter Josel, Dieter Heindl, Bernhard Irlinger, Christian Weilke
  • Publication number: 20090264618
    Abstract: Disclosed herein are branched linkers of the formula: Zn-Y-Xm Wherein X, Y, Z, m, and n are as defined herein. The branched linkers are useful for producing conjugates that are used in diagnostic or therapeutic methods. Methods of producing the branched linkers are also described.
    Type: Application
    Filed: February 13, 2009
    Publication date: October 22, 2009
    Inventors: Herbert Andres, Hans-Peter Josel, Eva Hoess, Rupert Herrmann, Herbert Von Der Eltz
  • Publication number: 20080305469
    Abstract: A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation.
    Type: Application
    Filed: April 22, 2008
    Publication date: December 11, 2008
    Inventors: Carina Horn, Hans-Peter Josel, Jurgen Spinke, Rupert Herrmann, Dieter Heindl
  • Publication number: 20080138914
    Abstract: A conjugate comprising a dye-labeled microporous crystal, a stop-cock moiety, and covalently bound thereto an affinity binding agent is disclosed. The dye-labeled microporous crystal is a zeolite crystal, such as a zeolite L crystal, having a large number of channels in its interior into which the dye is loaded. The stop-cock moiety can be functionalized with an amino group to which a carboxyester group can be attached. The affinity binding agent allows for the binding to a biological moiety. The conjugate of the moiety can be used in in-vivo and/or in-vitro imaging applications.
    Type: Application
    Filed: June 29, 2007
    Publication date: June 12, 2008
    Inventors: Zoran Popovic, Manuel Matthias Tsotsalas, Michael Busby, Luisa De Cola, Gion Calzaferri, Hans-Peter Josel
  • Patent number: 7378255
    Abstract: A method and reagent for detecting an analyte by a redox reaction and a fluorimetric determination, is disclosed. The method comprises contacting a sample containing the analyte with a detection reagent which contains a compound of the general formula Q-F as a fluorimetric redox indicator, wherein Q is a quencher group and F is a fluorophore group.
    Type: Grant
    Filed: January 28, 2004
    Date of Patent: May 27, 2008
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Carina Horn, Hans-Peter Josel, J├╝rgen Spinke, Rupert Herrmann, Dieter Heindl
  • Patent number: 7332354
    Abstract: The present invention relates to a chemical compound comprising a light emitting moiety precursor and a precursor of a leaving group, bound to each other by an amide or by an ester bond and characterized in that the leaving group precursor upon oxidation is converted into the leaving group. The invention also relates to compounds additionally comprising a coupling group to the use of such compounds for labeling of biomolecules and more generally to the use of such compounds in chemiluminescence detection procedures.
    Type: Grant
    Filed: May 28, 2002
    Date of Patent: February 19, 2008
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Dieter Heindl, Rupert Herrmann, Hans-Peter Josel, Erasmus Huber, Ursula Klause
  • Patent number: 7262306
    Abstract: The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays.
    Type: Grant
    Filed: January 6, 2006
    Date of Patent: August 28, 2007
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Wolfgang Jenni, Dieter Heindl, Rupert Herrmann, Hans-Peter Josel, Gunter Lampert
  • Publication number: 20070196852
    Abstract: The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.
    Type: Application
    Filed: February 16, 2007
    Publication date: August 23, 2007
    Inventors: Dieter Heindl, Frank Bergmann, Hans-Peter Josel, Dirk Kessler, Gunter Lampert, Christian Weilke
  • Publication number: 20060269922
    Abstract: The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
    Type: Application
    Filed: April 1, 2004
    Publication date: November 30, 2006
    Inventors: Gregor Sagner, Ingrid Bechler, Jochen Bolte, Dieter Heindl, Hans-Peter Josel, Martin Gutekunst, Rudolf Sebl, Christoph Mueller
  • Publication number: 20060199223
    Abstract: The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays.
    Type: Application
    Filed: January 6, 2006
    Publication date: September 7, 2006
    Applicant: Roche Diagnostics Operations, Inc.
    Inventors: Wolfgang Jenni, Dieter Heindl, Rupert Herrmann, Hans-Peter Josel, Gunter Lampert