Patents by Inventor Harold G. Craighead

Harold G. Craighead has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220056392
    Abstract: A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.
    Type: Application
    Filed: November 5, 2021
    Publication date: February 24, 2022
    Inventors: Thomas N. Corso, Harold G. Craighead
  • Publication number: 20220016629
    Abstract: A microfluidic chip for on-chip detection of the presence or absence of a target nucleic acid region in an isolated nucleic acid sample is disclosed. The microfluidic chip includes a nucleic acid entanglement array, an isolated nucleic acid sample immobilized in the nucleic acid entanglement array, and at least one probe specific to a target nucleic acid region. Systems and methods of using the microfluidic chip are disclosed.
    Type: Application
    Filed: November 29, 2019
    Publication date: January 20, 2022
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Harvey C. TIAN, David M. LIN, Adam J. BISOGNI
  • Publication number: 20220010360
    Abstract: A method for isolating one or more distinct analyte component from a cell sample is disclosed, as well as processes for testing and analyzing the distinct analyte components. The distinct analyte components include: (i) a total protein fraction; (ii) a plasma membrane protein fraction; (iii) a total RNA fraction; (iv) a cytosolic RNA fraction; (v) a cytosolic protein fraction; (vi) a nuclear RNA fraction; (vii) a nuclear protein fraction; (viii) a chromatin fraction comprising genomic DNA (gDNA); (ix) a gDNA markers fraction. This method involves the use of microfluidic device having a cell capture component and a nucleic acid entanglement component, where the cell capture component includes a cell capture array having a plurality of cell capture micropillars, where the nucleic acid entanglement component includes a nucleic acid entanglement array having a plurality of nucleic acid entanglement micropillars, and where the microfluidic device operates under continuous flow conditions.
    Type: Application
    Filed: November 29, 2019
    Publication date: January 13, 2022
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Harvey C. TIAN, David M. LIN, Adam J. BISOGNI
  • Publication number: 20210189381
    Abstract: The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.
    Type: Application
    Filed: March 6, 2021
    Publication date: June 24, 2021
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Juraj TOPOLANCIK, Harvey TIAN, Christopher WALLIN
  • Publication number: 20210178393
    Abstract: The present invention relates to, inter alia, a microfluidic device for capturing target cells and analyzing genomic DNA isolated from the target cells while under flow conditions. The microfluidic device includes a cell microchannel and a nucleic acid microchannel that intersect in an orthogonal manner, thereby forming a cell capture intersection region. The microfluidic device also includes a cell capture array and a nucleic acid entanglement array. The cell capture array includes a plurality of cell capturing micropillars and is located in the cell capture intersection region. The nucleic acid entanglement array includes a plurality of nucleic acid entanglement micropillars that function to physically entangle and maintain thereon genomic DNA isolated from the one or more target cell, and is located in a portion of the nucleic acid microchannel that is adjacent to and downstream of the cell capture intersection region. Methods of using the microfluidic device are also disclosed.
    Type: Application
    Filed: May 22, 2017
    Publication date: June 17, 2021
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Sarah J. REINHOLT
  • Patent number: 10947528
    Abstract: The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: March 16, 2021
    Assignee: CORNELL UNIVERSITY
    Inventors: Harold G. Craighead, Juraj Topolancik, Harvey Tian, Christopher Wallin
  • Publication number: 20200399578
    Abstract: Disclosed are a system, device, and method for the electroporation of cells. Systems, devices and methods for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The methods are particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells for use in cell-based therapies.
    Type: Application
    Filed: May 18, 2020
    Publication date: December 24, 2020
    Inventors: Tom Corso, Harold G. Craighead
  • Publication number: 20200316598
    Abstract: The present invention relates to, inter alia, a microfluidic device for performing single cell genomic DNA isolation and amplification under flow. The microfluidic device comprises a solid substrate having one or more microfluidic channel system formed therein. Each microfluidic channel system of the microfluidic device comprises: (a) an intake region comprising a single microchannel; (b) a plurality of cell segregation microchannels; (c) a cell capture site located downstream of each cell segregation microchannel; and (d) a DNA capture array positioned downstream of the cell capture site and comprising a plurality of micropillars. Also disclosed is a whole genome amplification system that includes the microfluidic device of the present disclosure, as well as a method for conducting single cell DNA analysis via on-chip whole genome amplification while under flow, and a method for multiple displacement amplification (MDA) reactions of one or more nucleic acid sequence isolated single cells.
    Type: Application
    Filed: May 22, 2017
    Publication date: October 8, 2020
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Harvey C. TIAN
  • Patent number: 10670559
    Abstract: An electrical detector is provided that comprises a nanofluidic channel with an integrated nanoscale charge sensor. The charge sensor can be an unfunctionalized nanowire, nanotube, transistor or capacitor and can be of carbon, silicon, carbon/silicon or other semiconducting material. The nanofluidic channel depth is on the order of the Debye screening length. Methods are also provided for detecting charged molecules or biological or chemical species with the electrical detector. Charged molecules or species in solution are driven through the nanofluidic channel of the electrical detector and contact the charge sensor, thereby producing a detectable signal. Methods are also provided for detecting a local solution potential of interest. A solution flowing through the nanofluidic channel of the electrical detector contacts the charge sensor, thereby producing a detectable local solution potential signal.
    Type: Grant
    Filed: July 9, 2009
    Date of Patent: June 2, 2020
    Assignee: Cornell University
    Inventors: John T. Mannion, Harold G. Craighead
  • Patent number: 10132798
    Abstract: The present invention relates to a microcolumn device for selecting nucleic acid aptamers for single and multiple target molecules, as well as a method for making the microcolumn device. The present invention also relates to a system for selecting nucleic acid aptamers for single and multiple target molecules. The present invention further relates to methods of using the microcolumn device for selecting nucleic acid aptamers for multiple target molecules. Kits that include one or more microcolumn device and/or system of the present invention are also disclosed.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: November 20, 2018
    Assignee: CORNELL UNIVERSITY
    Inventors: Harold G. Craighead, David R. Latulippe, John T. Lis, Abdullah Ozer, Kylan Szeto
  • Publication number: 20180305682
    Abstract: The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.
    Type: Application
    Filed: March 26, 2018
    Publication date: October 25, 2018
    Applicant: CORNELL UNIVERSITY
    Inventors: Harold G. CRAIGHEAD, Juraj TOPOLANCIK, Harvey TIAN, Christopher WALLIN
  • Patent number: 9988667
    Abstract: Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality of epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: June 5, 2018
    Assignee: Cornell University
    Inventors: Harold G. Craighead, Benjamin R. Cipriany, Stephen Levy, Paul Soloway
  • Patent number: 9926552
    Abstract: The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.
    Type: Grant
    Filed: June 6, 2012
    Date of Patent: March 27, 2018
    Assignee: CORNELL UNIVERSITY
    Inventors: Harold G. Craighead, Juraj Topolancik, Harvey Tian, Christopher Wallin
  • Patent number: 9856513
    Abstract: The present invention relates to methods and arrays for use in high resolution imaging of individual nucleic acid molecules and chromatin fragments, including native chromatin fragments. In one aspect, the present invention relates to a chromatin array that includes a transfer platform having a support and a transfer surface layered on the support. The chromatin array also includes a plurality of elongated individual native chromatin fragments coupled to the transfer surface in an orderly pattern suitable for high resolution imaging of the plurality of native chromatin fragments. The native chromatin fragments of the chromatin array include both DNA and histones.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: January 2, 2018
    Assignee: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Harvey C. Tian
  • Patent number: 9803192
    Abstract: The present invention generally relates to microcolumn affinity chromatography devices, systems that include the microcolumn affinity chromatography devices of the present disclosure, methods of using the devices and the systems of the present disclosure, and methods of making the devices and the systems of the present disclosure. In certain embodiments, the microcolumn affinity chromatography device is suitable for conducting affinity chromatography in multiple microcolumns in parallel and/or in series.
    Type: Grant
    Filed: October 6, 2014
    Date of Patent: October 31, 2017
    Assignee: CORNELL UNIVERSITY
    Inventors: Harold G. Craighead, Kylan Szeto, Sarah Reinholt, John T. Lis, Abdullah Ozer
  • Patent number: 9605298
    Abstract: Systems and methods are provided for high speed sorting of objects in a continuous body of fluid. The object can be analyzed within one or more interrogation volumes that allow for simultaneous or time-correlated measurement of the object's properties. A processor can interpret the properties of the object and then measured and then direct the object to one of a plurality of downstream flow paths. In some embodiments, the sorting of the object is based on two or more properties of the object. The sorting process can be repeated to create a network of sorting events.
    Type: Grant
    Filed: August 6, 2010
    Date of Patent: March 28, 2017
    Assignee: Cornell University
    Inventors: Harold G. Craighead, Benjamin R. Cipriany, Stephen Levy, Paul Soloway
  • Publication number: 20170073741
    Abstract: Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.
    Type: Application
    Filed: August 26, 2016
    Publication date: March 16, 2017
    Inventors: Harold G. Craighead, Benjamin R. Cipriany, Stephen Levy, Paul Soloway
  • Patent number: 9562888
    Abstract: A composite, analyte sensor includes a substrate; a micro- or nano-electro-mechanical (MEMS; NEMS) resonator that is coupled to the substrate at least two edge locations (i.e., it is at least doubly-clamped) of the resonator, wherein the resonator is in a statically-buckled state near a buckling transition point of the resonator; and a chemically-responsive substance covering at least a portion of the surface of the resonator that will undergo a conformational change upon exposure to a given analyte. The resonator may be a double-clamped, statically-buckled beam (or bridge), a multiply-clamped, statically-buckled dome (or crater), or other resonator geometry. The sensor may include two or more at least double-clamped, statically-buckled, composite MEMS or NEMS resonators each operating near a buckling transition point of the respective resonator, and each characterized by a different resonant frequency. A method for sensing an analyte in ambient air.
    Type: Grant
    Filed: March 31, 2011
    Date of Patent: February 7, 2017
    Assignee: CORNELL UNIVERSITY
    Inventors: Jeevak M. Parpia, Harold G. Craighead, Darren R. Southworth, Leon M. Bellan
  • Patent number: 9493022
    Abstract: An apparatus for forming an array of deposits on a substrate is disclosed. The apparatus may include a stencil capable of releasable attached to the substrate and having an array of openings and at least one alignment mark. The apparatus may further include a high throughput deposition printer aligned with the stencil to form an array of deposits on the substrate. The array of deposits may be aligned with the array of openings through the at least one alignment mark and an optional alignment device. Methods of manufacturing the stencil and using it to generate multiplexed or combinatorial arrays are also disclosed.
    Type: Grant
    Filed: August 5, 2010
    Date of Patent: November 15, 2016
    Assignee: Cornell University
    Inventors: Christine P. Tan, David M. Lin, Harold G. Craighead
  • Patent number: 9447451
    Abstract: Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.
    Type: Grant
    Filed: April 23, 2014
    Date of Patent: September 20, 2016
    Assignee: Cornell University
    Inventors: Harold G. Craighead, Benjamin R. Cipriany, Stephen Levy, Paul Soloway