Patents by Inventor Helen Franklin
Helen Franklin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117340Abstract: The invention related to forming nucleic add templates including control templates for sequencing using a nanopore-based method, wherein the templates of the novel structure disclosed herein are limited or prevented from threading into the nanopore during sequencing.Type: ApplicationFiled: February 18, 2021Publication date: April 11, 2024Inventors: Helen Franklin, Stanislav Fridland, Spencer Klugherz
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Patent number: 11891650Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.Type: GrantFiled: February 22, 2021Date of Patent: February 6, 2024Assignee: INTEGENX, INC.Inventors: David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich, Dennis Lehto, Kaiwan Chear, James Klevenberg, Chungsoo Charles Park, Corey M. Smith, Philip Justus Wunderle, David King
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Patent number: 11802276Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: GrantFiled: May 24, 2018Date of Patent: October 31, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Helen Franklin, Cynthia Cech, Timothy Kellogg Craig, Aruna Ayer, Kirti Dhiman, Natalie B. Chechelski Johnston, Joshua N. Mabry, Arkadiusz Bibillo, Peter Crisalli, Randall W. Davis
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Patent number: 11746337Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: GrantFiled: May 11, 2020Date of Patent: September 5, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Helen Franklin, Cynthia Cech, Timothy Kellogg Craig, Aruna Ayer, Kirti Dhiman, Natalie B. Chechelski Johnston, Joshua N. Mabry, Arkadiusz Bibillo, Peter Crisalli, Randall W. Davis
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Publication number: 20230159999Abstract: This application discloses compositions comprising primer compounds that reduce or block deleterious threading into a nanopore of nucleic acid strands displaced by a nanopore-linked polymerase, for example during the use of a nanopore device for nucleic acid sequencing. Also disclosed are methods for using the compositions to reduce deleterious threading events during nanopore-based nucleic acid detection techniques, such as nanopore sequencing.Type: ApplicationFiled: August 4, 2022Publication date: May 25, 2023Inventors: Aruna Ayer, David Beyer, Peter Crisalli, Kirti Dhiman, Helen Franklin, Swathi Chakravarthy, Omid Khakshoor, Blake Langdon, Meng Taing, Adolfo Vargas
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Patent number: 11525124Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: GrantFiled: May 11, 2020Date of Patent: December 13, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Helen Franklin, Cynthia Cech, Timothy Kellogg Craig, Aruna Ayer, Kirti Dhiman, Natalie B. Chechelski Johnston, Joshua N. Mabry, Arkadiusz Bibillo, Peter Crisalli, Randall W. Davis
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Publication number: 20210381041Abstract: The present invention provides a method for isolating Sequencing complexes, said method comprising forming a complex between a nanopore covalently linked to a polymerase and an oligonucleotide that is associated with a purification moiety, separating any unbound/uncomplexed nanopores and oligonucleotides from the complexes by use of a solid support capable of binding the purification moiety, and cleaving bound complexes from the solid support with an enzyme composition.Type: ApplicationFiled: May 27, 2019Publication date: December 9, 2021Inventors: Kirti DHIMAN, Helen FRANKLIN, Alexandra WANG
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Publication number: 20210278427Abstract: Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.Type: ApplicationFiled: March 24, 2021Publication date: September 9, 2021Inventors: James Ogg, David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich
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Publication number: 20210180114Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.Type: ApplicationFiled: February 22, 2021Publication date: June 17, 2021Inventors: David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich, Dennis Lehto, Kaiwan Chear, James Klevenberg, Chungsoo Charles Park, Corey M. Smith, Philip Justus Wunderle, David King
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Patent number: 10989723Abstract: Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.Type: GrantFiled: January 25, 2019Date of Patent: April 27, 2021Assignee: IntegenX, Inc.Inventors: James Ogg, David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich
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Patent number: 10961561Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.Type: GrantFiled: January 17, 2019Date of Patent: March 30, 2021Assignee: IntegenX, Inc.Inventors: David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich, Dennis Lehto, Kaiwan Chear, James Klevenberg, Chungsoo Charles Park, Corey M. Smith, Philip Justus Wunderle, David King
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Publication number: 20200277581Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: ApplicationFiled: May 11, 2020Publication date: September 3, 2020Applicant: Roche Sequencing Solutions, Inc.Inventors: Helen FRANKLIN, Cynthia CECH, Timothy Kellogg CRAIG, Aruna AYER, Kirti DHIMAN, Natalie B. CHECHELSKI JOHNSTON, Joshua N. MABRY, Arkadiusz BIBILLO, Peter CRISALLI, Randall W. DAVIS
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Publication number: 20200270589Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: ApplicationFiled: May 11, 2020Publication date: August 27, 2020Applicant: ROCHE SEQUENCING SOLUTIONS, INC.Inventors: Helen FRANKLIN, Cynthia CECH, Timothy Kellogg CRAIG, Aruna AYER, Kirti DHIMAN, Natalie B. CHECHELSKI JOHNSTON, Joshua N. MABRY, Arkadiusz BIBILLO, Peter CRISALLI, Randall W. DAVIS
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Publication number: 20190256890Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.Type: ApplicationFiled: January 17, 2019Publication date: August 22, 2019Inventors: David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich, Dennis Lehto, Kaiwan Chear, James Klevenberg, Chungsoo Charles Park, Corey M. Smith, Philip Justus Wunderle, David King
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Publication number: 20190242917Abstract: Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.Type: ApplicationFiled: January 25, 2019Publication date: August 8, 2019Inventors: James Ogg, David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich
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Patent number: 10208332Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.Type: GrantFiled: April 30, 2015Date of Patent: February 19, 2019Inventors: David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich, Dennis Lehto, Kaiwan Chear, James Klevenberg, Chungsoo Charles Park, Corey Smith, Philip Justus Wunderle, David King
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Patent number: 10191071Abstract: Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.Type: GrantFiled: November 17, 2014Date of Patent: January 29, 2019Assignee: IntegenX, Inc.Inventors: James Ogg, David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich
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Publication number: 20180340157Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: ApplicationFiled: May 24, 2018Publication date: November 29, 2018Inventors: Helen FRANKLIN, Cynthia CECH, Timothy Kellogg CRAIG, Aruna AYER, Kirti DHIMAN, Natalie B. CHECHELSKI JOHNSTON, Joshua N. MABRY, Arkadiusz BIBILLO, Peter CRISALLI, Randall W. DAVIS
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Publication number: 20170002399Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.Type: ApplicationFiled: April 30, 2015Publication date: January 5, 2017Inventors: David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich, Dennis Lehto, Kaiwan Chear, David King, James Klevenberg, Chungsoo Charles Park, Corey Smith, Philip Justus Wunderle
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Publication number: 20160305972Abstract: Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.Type: ApplicationFiled: November 17, 2014Publication date: October 20, 2016Applicant: IntegenX Inc.Inventors: James Ogg, David Eberhart, William D. Nielsen, Helen Franklin, Stevan B. Jovanovich