Abstract: A device for extraction or isolation of an analyte, such as a nucleic acid, a protein, or a cell, from a sample, and in particular from a biological sample, is described. Methods of using the device are also described. Further processes, such as amplification of the isolated analyte, may also be carried out within the device.

Abstract: The invention in some embodiments relates to systems and methods of controlling pressure within a prosthetic knee. The knee in one embodiment comprises a flexible diaphragm that substantially prevents or mitigates undesirable pressure build-up in a sealed chamber of the knee. The knee in another embodiment comprises a compressible gas in the chamber that substantially prevents or mitigates undesirable pressure build-up in the chamber.

Abstract: The invention in some embodiments relates to a dynamic seal for a prosthetic knee. The dynamic seal in one embodiment is utilized to seal a magnetorheological fluid comprising a liquid and solid particles within a chamber of the knee. The dynamic seal embodiments are specially configured with a pre-loaded tensioned garter spring which has a coil spacing that is at least as large as the size of the particles or maximum size of the particles in the magnetorheological fluid. Desirably, this allows the magnetorheological fluid particles to flow in and out of the dynamic seal without clogging the seal and advantageously provides for a reliable dynamic seal.

Abstract: The present invention provides methods for treating individuals having a hepatitis C virus (HCV) infection, which individuals have failed to respond to therapy with an IFN-? other than consensus interferon (CIFN), or who, following cessation of therapy with an IFN-? other than CIFN, have suffered relaspe. The methods generally involve a treatment regimen comprising administering a first dosing regimen of CIFN, followed by a second dosing regimen of CIFN. Ribavirin is administered with at least the second dosing regimen.

Abstract: The invention in some embodiments relates to systems and methods of loading fluid in a prosthetic knee. A vacuum fill technique is utilized in one embodiment to load magnetorheological fluid within small gaps in a chamber of a prosthetic knee. Advantageously, this allows for an efficient loading scheme thereby desirably allowing for faster manufacturing speed. Another advantage is that the magnetorheological fluid is substantially uniformly distributed within the small gaps thereby desirably providing consistent production.

Abstract: The present invention provides methods of reducing liver fibrosis; methods of increasing liver function in an individual suffering from liver fibrosis; and methods of reducing the incidence of complications associated with cirrhosis of the liver. The methods generally involve administering a therapeutically effective amount of IFN-&ggr;.