Patents by Inventor Hideki Sawai

Hideki Sawai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200325097
    Abstract: Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 ?m and less than 1 ?m with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.
    Type: Application
    Filed: May 14, 2020
    Publication date: October 15, 2020
    Inventors: Kenji Sawai, Hideki Sawai, Takashi Mimitsuka, Masateru Ito, Katsushige Yamada, Kenji Kawamura, Shin-ichi Minegishi, Izumi Na kagawa, Tatsuya Nagano
  • Patent number: 10683254
    Abstract: Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 ?m and less than 1 ?m with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.
    Type: Grant
    Filed: December 25, 2009
    Date of Patent: June 16, 2020
    Assignee: Toray Industries, Inc.
    Inventors: Kenji Sawai, Hideki Sawai, Takashi Mimitsuka, Ito Masateru, Katsushige Yamada, Kenji Kawamura, Shin-ichi Minegishi, Izumi Nakagawa, Tatsuya Nagano
  • Patent number: 10428358
    Abstract: A method of producing a chemical product includes cultivating microorganisms or culture cells in a fermentation tank; transferring a culture liquid from the fermentation tank to a membrane separation tank to filter the culture liquid through a separation membrane; and collecting a fermentation product from a filtration liquid as the chemical product while refluxing an unfiltered culture liquid that has not been filtered to be joined to the culture liquid on an upstream side of the membrane separation tank, wherein one portion of the culture liquid to be transferred from the fermentation tank is allowed to bypass the membrane separation tank depending on a pressure at a culture liquid flow-in side of the membrane separation tank.
    Type: Grant
    Filed: February 24, 2016
    Date of Patent: October 1, 2019
    Assignee: Toray Industries, Inc.
    Inventors: Takashi Mimitsuka, Kentaro Ishii, Ken Morita, Masashi Higasa, Kenji Sawai, Hideki Sawai, Katsushige Yamada, Shinichi Minegishi
  • Patent number: 10093747
    Abstract: A method of producing a sugar liquid using a cellulose-containing biomass as a raw material includes (a) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution and (b) filtering the obtained aqueous sugar solution through a reverse osmosis membrane to collect a purified sugar liquid from a feed side, while removing fermentation-inhibiting substances from a permeate side.
    Type: Grant
    Filed: May 28, 2014
    Date of Patent: October 9, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
  • Patent number: 9644221
    Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: May 9, 2017
    Assignee: Toray Industries, Inc.
    Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
  • Patent number: 9587253
    Abstract: The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 ?m or more to less than 1 ?m is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost.
    Type: Grant
    Filed: February 16, 2007
    Date of Patent: March 7, 2017
    Assignee: Toray Industries, Inc.
    Inventors: Hideki Sawai, Katsushige Yamada, Takashi Mimitsuka, Kenji Sawai, Tetsu Yonehara, Yohito Ito, Masahiro Henmi
  • Publication number: 20160168601
    Abstract: A method of producing a chemical product includes cultivating microorganisms or culture cells in a fermentation tank; transferring a culture liquid from the fermentation tank to a membrane separation tank to filter the culture liquid through a separation membrane; and collecting a fermentation product from a filtration liquid as the chemical product while refluxing an unfiltered culture liquid that has not been filtered to be joined to the culture liquid on an upstream side of the membrane separation tank, wherein one portion of the culture liquid to be transferred from the fermentation tank is allowed to bypass the membrane separation tank depending on a pressure at a culture liquid flow-in side of the membrane separation tank.
    Type: Application
    Filed: February 24, 2016
    Publication date: June 16, 2016
    Inventors: Takashi Mimitsuka, Kentaro Ishii, Ken Morita, Masashi Higasa, Kenji Sawai, Hideki Sawai, Katsushige Yamada, Shinichi Minegishi
  • Patent number: 9080190
    Abstract: A method produces cadaverine more efficiently and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having an ability to produce cadaverine and having a resistance to 2,2?-thiobis(ethylamine). Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: July 14, 2015
    Assignee: Toray Industries, Inc.
    Inventors: Nanami Sasaki, Takashi Mimitsuka, Hideki Sawai, Kenji Sawai
  • Patent number: 9018424
    Abstract: A method for producing a diol or triol, which has a step of removing impurities contained in a diol- or triol-containing solution, is provided. In the method, a diol- or triol-containing solution is filtered through a nanofiltration membrane having a polyamide-containing functional layer. The diol- or triol-containing solution is then collected from the permeate flow of the nanofiltration membrane.
    Type: Grant
    Filed: March 30, 2011
    Date of Patent: April 28, 2015
    Assignee: Toray Industries, Inc.
    Inventors: Izumi Morita, Masateru Ito, Hideki Sawai, Shinichi Minegishi, Katsushige Yamada, Kenji Kawamura, Shinichi Yamane
  • Patent number: 8999681
    Abstract: A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: April 7, 2015
    Assignee: Toray Industries, Inc.
    Inventors: Nanami Sasaki, Takashi Mimitsuka, Hideki Sawai, Kenji Sawai
  • Publication number: 20150056665
    Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 26, 2015
    Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
  • Patent number: 8871477
    Abstract: By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: October 28, 2014
    Assignee: Toray Industries, Inc.
    Inventors: Takashi Mimitsuka, Kazumi Suda, Hideki Sawai
  • Patent number: 8859260
    Abstract: A gene expressing cassette codes lactate dehydrogenase that is needed for prevention of deterioration in lactic acid yield and lactic acid production rate in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability, which achieves high optical purity, high lactic acid yield and high lactic acid production rate simultaneously, a yeast strain having the cassette and a method of producing lactic acid by culturing the yeast strain. The lactate dehydrogenase-expressing cassette is a lactate dehydrogenase-expressing cassette, comprising a gene coding lactate dehydrogenase connected to a site downstream of a promoter, the promoter being a promoter of a gene showing a gene expression amount larger by 5 times or more than the average relative expression amount of all genes after 50 hours from start of culture in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability.
    Type: Grant
    Filed: December 5, 2008
    Date of Patent: October 14, 2014
    Assignee: Toray Industries, Inc.
    Inventors: Kenji Sawai, Hideki Sawai
  • Publication number: 20140287461
    Abstract: A method of producing a sugar liquid using a cellulose-containing biomass as a raw material includes (a) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution and (b) filtering the obtained aqueous sugar solution through a reverse osmosis membrane to collect a purified sugar liquid from a feed side, while removing fermentation-inhibiting substances from a permeate side.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 25, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
  • Patent number: 8822195
    Abstract: Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.
    Type: Grant
    Filed: June 2, 2010
    Date of Patent: September 2, 2014
    Assignee: Toray Industries, Inc.
    Inventors: Kenji Sawai, Kazumi Suda, Hideki Sawai, Katsushige Yamada, Junya Yamagishi
  • Patent number: 8765405
    Abstract: A sugar liquid containing only very small amounts of fermentation-inhibiting substances is produced by a method for producing a sugar liquid using a cellulose-containing biomass as a raw material, the method including: (1) a step of hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution; and (2) a step of filtering the obtained aqueous sugar solution through a nanofiltration membrane and/or reverse osmosis membrane to collect a purified sugar liquid from the feed side, while removing fermentation-inhibiting substances from the permeate side.
    Type: Grant
    Filed: December 8, 2009
    Date of Patent: July 1, 2014
    Assignee: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
  • Patent number: 8759045
    Abstract: A method of producing lactic acid by separating lactic acid produced in a culture solution by microbial fermentation, comprising: a step (A) of filtering the culture solution through a nano-filtration membrane; and a step (B) of distilling a lactic-acid-containing solution produced in step (A) under a pressure of 1 Pa to atmospheric pressure (inclusive) at a temperature of 25 to 200° C. (inclusive) to recover lactic acid.
    Type: Grant
    Filed: December 7, 2012
    Date of Patent: June 24, 2014
    Assignee: Toray Industries, Inc.
    Inventors: Masateru Ito, Shin-ichi Minegishi, Eri Shimizu, Kenji Sawai, Yohito Ito, Hideki Sawai, Katsushige Yamada
  • Patent number: 8669093
    Abstract: A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector.
    Type: Grant
    Filed: September 17, 2004
    Date of Patent: March 11, 2014
    Assignee: Mitsui Chemicals, Inc.
    Inventors: Mitsufumi Wade, Toshihiro Oikawa, Daisuke Mochizuki, Junko Tokuda, Miyuki Kawashima, Tadashi Araki, Reiko Abe, Hitoki Miyake, Hitoshi Takahashi, Hideki Sawai, Takashi Mimitsuka, Takashi Morishige, Yosuke Higashi
  • Publication number: 20140017746
    Abstract: A method of producing lactic acid by continuous fermentation includes filtering a culture of polyploidy prototrophic yeast having a capacity to produce lactic acid by introduction of a lactate dehydrogenase gene through a porous membrane having an average pore size of not less than 0.01 ?m and less than 1 ?m; and recovering the lactic acid from filtrate while the unfiltered liquid is retained in or returned to the culture and a fermentation feedstock is added to the culture.
    Type: Application
    Filed: September 16, 2013
    Publication date: January 16, 2014
    Applicant: TORAY INDUSTRIES, INC.
    Inventors: Nanami Sasaki, Ken Morita, Takashi Mimitsuka, Hideki Sawai, Katsushige Yamada
  • Publication number: 20140004576
    Abstract: A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.
    Type: Application
    Filed: December 8, 2011
    Publication date: January 2, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Nanami Sasaki, Takashi Mimitsuka, Hideki Sawai, Kenji Sawai