Patents by Inventor Hidetaka Uno

Hidetaka Uno has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10208284
    Abstract: Provided is a device for seeding cells in a plurality of cell arrangement areas in a simple manner and a short period of time. A seeding and culturing device (1) for cells capable of forming a nerve network, the device comprising a cell-culturing substrate (2) having a plurality of cell arrangement areas (8) enclosed by a plurality of projecting parts, and a flow channel substrate (3) arranged on the cell-cultivating substrate (2) and having a plurality of through-holes (14), wherein the through-holes (14) are configured so as to provide flow channels in which the upper surface side of the substrate is an entrance (15) and the lower surface side of the substrate is an exit, and the exit (16) of the flow channels is positioned above any of the cell arrangement areas.
    Type: Grant
    Filed: January 23, 2015
    Date of Patent: February 19, 2019
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Yasutaka Nagaoka
  • Patent number: 10114005
    Abstract: An object of the present invention is that variations in an applied membrane potential in the planar patch clamp device are suppressed to reduce a noise current, thereby enabling accurate measurement of an ion channel current. Disclosed is a planar patch clamp device including: an electrically insulative substrate provided with one or more fine through holes; a liquid reservoir that holds a conductive liquid provided on both surface sides of the through hole; and energizable electrode sections provided in the liquid reservoir; these electrode sections including: (a) an electrode vessel, at least part of which is made of an inorganic porous material, (b) an electrode in which a chloride NmCl layer is formed on the surface of a noble metal Nm, and (c) an electrode solution containing NmCl and an alkali metal chloride being dissolved therein at a saturated concentration.
    Type: Grant
    Filed: December 5, 2012
    Date of Patent: October 30, 2018
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Senthil Kumar Obuliraj, Yasutaka Nagaoka
  • Patent number: 9977009
    Abstract: A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid; a second reservoir (6?) provided at the second surface side (2S?) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7?) arranged to be able to be electrically conductive with the second reservoir (6?) through the second conductive liquid; a supply path (8) connected to the second reservoir (6?) and
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: May 22, 2018
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Yasutaka Nagaoka, Kei Kobayashi
  • Patent number: 9829477
    Abstract: A planar patch clamp device is disclosed, which can be used for culturing a neuron in the device so as to form a neuron network, and detecting an electrical property of the neuron that forms the neuron network. The planar patch clamp device includes a plurality of protrusions formed on a first surface, an extracellular matrix forming substance which is coated on the peripheries of a through hole, and electrode sections.
    Type: Grant
    Filed: March 21, 2013
    Date of Patent: November 28, 2017
    Assignee: Japan Science and Technology Agency
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Miho Saitoh, Yasutaka Nagaoka
  • Publication number: 20170002315
    Abstract: Provided is a device for seeding cells in a plurality of cell arrangement areas in a simple manner and a short period of time. A seeding and culturing device (1) for cells capable of forming a nerve network, the device comprising a cell-culturing substrate (2) having a plurality of cell arrangement areas (8) enclosed by a plurality of projecting parts, and a flow channel substrate (3) arranged on the cell-cultivating substrate (2) and having a plurality of through-holes (14), wherein the through-holes (14) are configured so as to provide flow channels in which the upper surface side of the substrate is an entrance (15) and the lower surface side of the substrate is an exit, and the exit (16) of the flow channels is positioned above any of the cell arrangement areas.
    Type: Application
    Filed: January 23, 2015
    Publication date: January 5, 2017
    Applicant: Japan Science and Technology Agency
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Yasutaka Nagaoka
  • Publication number: 20160209395
    Abstract: A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid; a second reservoir (6?) provided at the second surface side (2S?) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7?) arranged to be able to be electrically conductive with the second reservoir (6?) through the second conductive liquid; a supply path (8) connected to the second reservoir (6?) a
    Type: Application
    Filed: August 29, 2014
    Publication date: July 21, 2016
    Applicant: Japan Science and Technology Agency
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Yasutaka Nagaoka, Kei Kobayashi
  • Publication number: 20150233890
    Abstract: Provided are a culture device for neuronal network formation that makes it possible to construct a neuronal network while restricting movement of neurons grown in the culture medium, and a means for the use thereof. A culture device for neuronal network formation in which is formed a cell fixation part surrounded by a plurality of projections on a flat substrate that can be filled with cell culture medium, and in which (1) there are established spaces having widths such that the neuron cells cannot pass through between the plurality of projections, (2) the inner diameter is such that the cell fixation part can house the cells of from one to a number of neurons, (3) the substrate surface, which is the bottom of the cell fixation part, is coated by an extracellular matrix-forming substance and/or provided with minute through-holes for suctioning culture medium by a suction device on the lower side of the substrate surface.
    Type: Application
    Filed: March 21, 2013
    Publication date: August 20, 2015
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Miho Saitoh, Yasutaka Nagaoka
  • Publication number: 20140339102
    Abstract: An object of the present invention is that variations in an applied membrane potential in the planar patch clamp device are suppressed to reduce a noise current, thereby enabling accurate measurement of an ion channel current. Disclosed is a planar patch clamp device including: an electrically insulative substrate provided with one or more fine through holes; a liquid reservoir that holds a conductive liquid provided on both surface sides of the through hole; and energizable electrode sections provided in the liquid reservoir; these electrode sections including: (a) an electrode vessel, at least part of which is made of an inorganic porous material, (b) an electrode in which a chloride NmCl layer is formed on the surface of a noble metal Nm, and (c) an electrode solution containing NmCl and an alkali metal chloride being dissolved therein at a saturated concentration.
    Type: Application
    Filed: December 5, 2012
    Publication date: November 20, 2014
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Senthil Kumar Obuliraj, Yasutaka Nagaoka