Abstract: A production method can produce alkaloids at higher efficiencies than conventional methods includes expressing, in a Catharanthus plant of the family Apocynaceae and using gene manipulation, a protein that particulates in tryptophan biosynthesis and a protein that participates in secologanin biosynthesis.

Abstract: The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

Abstract: The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

Abstract: The object of the present invention is to provide a transformed plant having an enhanced amino acid content, a progeny of the plant, seeds of the plant and a method for producing the plant. The present invention provides a transformed plant having a gene encoding DNA binding protein of Dof family and also having a free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions. The present invention also provides a method of producing a transformed plant having the free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions by introducing a gene encoding DNA binding protein of Dof family into the plant to express the gene in the plant body.

Abstract: The object of the present invention is to provide transgenic plants which accumulate free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in the edible parts thereof, and a method of producing them are provided. The other object of the present invention is to provide a method of increasing the yielding of potato and to provide a transgenic potato of which yielding can be increased. In the present invention, a sequence encoding glutamate dehydrogenase (GDH) gene is introduced into a plant together with a suitable regulatory sequence to express it in a plant cell, and thereby the GDH gene is excessively expressed.

Abstract: The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

Abstract: The present invention provides a method of producing a plant which exhibits improved growth and/or yield under reduced nitrogen conditions, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by increasing 2-OG content in plants. Particularly, the present invention provides a method of producing a plant which exhibits improved growth and/or yield under conditions where nitrogen is reduced, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by introducing a GDH gene or ECASPC gene into plants and expressing the transgene GDH or ECAPS in the plants, which results in increased 2-OG, or by spraying proline on the leaves of plants to increase the 2-OG content, thereby enhancing the incorporation of nitrogen or metabolic activity of plants. Also provided is a method of cultivating such plants under nitrogen-limited conditions.

Abstract: Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

Abstract: The present invention provides a plant having a decreased dormancy period, a plant having a decreased dormancy period and uniform budding and/or germination timing, and a method for producing these plants. The intracellular 2-OG content in the plant may be increased, for example, by overexpressing an intracellular GDH gene in the plant. More specifically, the intracellular 2-OG content in the plant may be increased due to the overexpression of the GDH gene, by introducing into the plant a nucleic acid construct capable of enhancing expression of the GDH gene, particularly a nucleic acid construct capable of expressing the GDH gene.

Abstract: The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

Abstract: The object of the present invention is to provide transgenic plants which accumulate free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in the edible parts thereof, and a method of producing them are provided. The other object of the present invention is to provide a method of increasing the yielding of potato and to provide a transgenic potato of which yielding can be increased. In the present invention, a sequence encoding glutamate dehydrogenase (GDH) gene is introduced into a plant together with a suitable regulatory sequence to express it in a plant cell, and thereby the GDH gene is excessively expressed.

Abstract: Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

Abstract: Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate decarboxylase(GAD) gene is introduced into a plant in anti-sense direction together with an appropriate regulatory sequence, and the expression of GAD gene is supressed.

Abstract: The object of the present invention is to provide a transformed plant having an enhanced amino acid content, a progeny of the plant, seeds of the plant and a method for producing the plant. The present invention provides a transformed plant having a gene encoding DNA binding protein of Dof family and also having a free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions. The present invention also provides a method of producing a transformed plant having the free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions by introducing a gene encoding DNA binding protein of Dof family into the plant to express the gene in the plant body.

Abstract: The present invention provides a transformed plant having free-amino acid content increased by introducing phosphoenolpyruvate carboxylase (PEPC) genes, and also a progeny thereof and a seed thereof. Namely, the transformed plant has a nucleic acid construct containing PEPC gene introduced therein, wherein phosphoenolpyruvate carboxylase encoded by the PEPC gene does not require phosphorylation for the activation thereof and/or is independent of acetyl CoA. In particular, the present invention provides a transformed plant comprising a nucleic acid construct containing a nucleic acid molecule encoding Synechococcus vulcanus PEPC or a nucleic acid construct containing a nucleic acid molecule encoding a protein having PEPC activity which has at least 80% of nucleotide sequence homology to said gene.

Abstract: Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided.

Abstract: Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.