Abstract: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.

Abstract: Disclosed is an interphone system that uses a home-use television broadcast receiving apparatus capable of receiving instructions for dealing with a visitor. According to the invention, a television includes a sending/receiving section for sending and receiving signals to and from a door phone apparatus, a storage section in which response information is stored as text data, and an audio synthesis processor which converts text data into an audio signal. An audio signal based on response information (e.g., “Please come in”) selected with the television is sent to the door phone apparatus and outputted from a door phone slave unit installed at the entrance of a house. Thus, it becomes possible to deal with a visitor by operating the television.

Abstract: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.

Abstract: An aqueous resin composition comprising prepared by a process comprising the steps of: reacting epoxy resins comprising: (A) an aromatic epoxy resin having a number average molecular weight of at least 9,000 and an epoxy equivalent of not larger than 9,000; and (B) an aromatic epoxy resin having a number average molecular weight of less than 9,000 and an epoxy equivalent of not larger than 5,000, and (C) a carboxyl group-containing acrylic resin having a glass transition temperature of at least 100° C., to undergo partial-esterification to obtain an acryl-modified epoxy resin; neutralizing said acryl-modified epoxy resin with a base and dispersing the neutralized resin in an aqueous medium. Also disclosed is a coated metal material having a cured coated film of the aqueous resin composition.

Abstract: An aqueous resin composition comprising prepared by a process comprising the steps of: reacting epoxy resins comprising: (A) an aromatic epoxy resin having a number average molecular weight of at least 9,000 and an epoxy equivalent of not larger than 9,000; and (B) an aromatic epoxy resin having a number average molecular weight of less than 9,000 and an epoxy equivalent of not larger than 5,000, and (C) a carboxyl group-containing acrylic resin having a glass transition temperature of at least 100° C, to undergo partial-esterification to obtain an acryl-modified epoxy resin; neutralizing said acryl-modified epoxy resin with a base and dispersing the neutralized resin in an aqueous medium. Also disclosed is a coated metal material having a cured coated film of the aqueous resin composition.

Abstract: A method for manufacturing an insert-resin-molded product prevents resin burrs from being generated. The method includes the steps of: preparing a resin-molding mold having a cavity having an inner wall including a recess formed therein; preparing a metallic member to be placed in the cavity of the resin-molding mold so as to be integrally molded with resin, the member having a metallic part to be fit into a recess of the resin-molding mold; placing the metallic member in the cavity of the resin-molding mold and fitting the metallic part of the metallic member into the recess of the resin-molding mold; and injecting resin into the cavity of the resin-molding mold to integrally insert-resin-mold with the metallic member.

Abstract: A method for manufacturing an insert-resin-molded product prevents resin burrs from being generated. The method includes the steps of: preparing a resin-molding mold having a cavity having an inner wall including a recess formed therein; preparing a metallic member to be placed in the cavity of the resin-molding mold so as to be integrally molded with resin, the member having a metallic part to be fit into a recess of the resin-molding mold; placing the metallic member in the cavity of the resin-molding mold and fitting the metallic part of the metallic member into the recess of the resin-molding mold; and injecting resin into the cavity of the resin-molding mold to integrally insert-resin-mold with the metallic member.

Abstract: A coaxial connector receptacle which includes a casing, a hole which is formed in the casing and into which a plug with a conductor is to be inserted, a conductive protrusion provided in the hole for the purpose of receiving the conductor when the plug is inserted into the hole, and an access terminal extending from the protrusion along the bottom surface of the casing, wherein the access terminal is embedded in the casing member such that it is exposed so as to allow access from outside. Since the access terminal is embedded in the wall surface of the casing, the receptacle can be made compact in size.

Abstract: A neutral protease gene of Bacillus amyloliquefaciens is cloned, which gene comprises the promoter region, the ribosome binding region, the region involved in the secretion of the neutral protease, the region consisting of the structural gene for the neutral protease, and the terminator region. Each of the regions is useful as a material for construction of a recombinant DNA used for the production of proteins by culturing a host bacterium transformed with the recombinant DNA. For example, the extracellular production of neutral protease in a large amount can be accomplished by culturing B. subtilis transformed with a recombinant DNA comprising pUB110 and the neutral protease gene.

Abstract: A method of joining end portions of a weatherstrip consists of introducing the end portions to openings in a mold such that a seal portion of the weatherstrip is compressed substantially to the shape of a seal lip of the seal portion. The end portions are separated in the mold by a predetermined gap and a rubber material is injected into the gap for mutually bonding the end portions of the weatherstrip. Such a method of joining allows the weatherstrip to be installed on complexly shaped openings with no degradation of sealing characteristics over the perimeter covered by the weatherstrip. A molding apparatus for joining said end portions includes a clamping member for assuring correct positioning of a sealing edge of the weatherstrip.

Abstract: Residual extracellular protease activities of a Bacillus subtilis strain can be further reduced by introducing a gene for the stimulation of extracellular protease levels into the genomic DNA of the strain.The resultant strain whose extracellular protease activities are further reduced is transformed with a recombinant plasmid for secretion of a desired protein and the desired protein can be efficiently produced and accumulated in the culture medium in a large amount. The accumulated desired protein can be easily recovered from the culture medium.For example, the accumulation level of human growth hormone secreted by a B. subtilis MT-430 strain carrying recombinant DNA phGH427 for the expression and secretion of human growth hormone can be increased five to tenfold as a result of the above treatment for reduction of the residual extracellular protease activities of the extracellular protease-activity-deficient strain.

Abstract: A expression-secretion vector is constructed by using a DNA sequence comprising a DNA fragment obtained by subjecting a DNA sequence consisting of a region involved in the expression of a gene coding for an extracellular enzyme of a bacterium of the genus Bacillus and a region involved in the secretion of the enzyme thus expressed to a deletion in the region involved in the secretion which can enhance the extracellular production of a protein dependent on the DNA fragment. By transforming a host bacterium with a recombinant DNA molecule formed by inserting a DNA fragment comprising a gene coding for a desired protein into the expression-secretion vector and then culturing the transformed host, the extracellular production of the desired protein in a large amount can be accomplished.

Abstract: An aqueous coating resin composition, the improvement wherein a microgel composed of a resin reaction product having a three-dimensional network structure, which is obtained by the esterification reaction of (1) 40 to 90 parts by weight of an aromatic epoxy resin with (2) 10 to 60 parts by weight of a carboxyl group-containing vinyl polymer comprising (a) a carboxyl group-containing vinyl monomer, (b) an aromatic vinyl monomer and (c) at least one monomer selected from the group consisting of an alkyl ester, a hydroxyalkyl ester and an N-hydroxyalkylamide of an alpha,beta-ethylenically unsaturated carboxylic acid, is dispersed into an aqueous medium; and a process for production thereof.

Abstract: This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.

Abstract: A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperature-sensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is introduced into the host. The host is then cultured at a temperature which does not completely inhibit chromosomal DNA replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.

Abstract: A twist roller guide having a minimized overall length, including a guide box, a twist guide member having a twisted through hole for giving preliminary twist to the stock passing therethrough, and a roller holder consisting of a hollow cylindrical body portion and a pair of bearing plates extending forwardly therefrom to support a pair of twist rollers therebetween. The twist guide member consists of an enlarged head portion defining an inlet opening therein and a tubular body portion, the head portion being fitted in a bracket portion of the guide box formed adjacent to the rear end thereof. The body portion of the twist guide is fitted in the hollow cylindrical body portion of the roller holder, which is firmly supported in another bracket portion of the guide box formed adjacent to the front end thereof. The twist guide is beveled at the top and bottom of its exit end face so that it may be placed quite close to the twist rollers.

Abstract: A mechanism for automatically centering sub-guide rollers and adjusting the clearance between the sub-guide rollers thereby to adjust the clamping condition of the material to be rolled, for use in the material guiding apparatus of a rolling mill for rolling a steel blank into wires, bars and so forth, the material guiding apparatus being of a type having a pair of roller holders 2, 3 each of which carrying corresponding one of a pair of main guide rollers 4, 5 and a pair of sub-guide rollers 41, 51. Each of the sub-guide rollers is rotatably carried by corresponding one of a pair of eccentric shafts pins 26 unitary with each of a pair of support shafts 25 supported by a corresponding roller holder. A right hand screw gear 27 and a left hand screw gear 28 are attached to respective ones of the support shafts 25. A manually operable right hand screw worm 29 and a right hand screw worm 30 which is also manually operable are made to engage with the gears 27, 28, respectively.

Abstract: In a roller type stock guide of the type including, among others, a cylindrical nut member having threaded connections of opposite hands with a pair of threaded shafts and, being arranged between the two roller holder levers, operable to turn the latter in opposite directions thereby to vary the distance between the guide rollers, a ratchet type nut driving mechanism is provided which includes a ratchet wheel formed around the cylindrical nut integrally therewith, a pair of ratchet pawls movable into and out of meshing engagement with the ratchet wheel in alternate fashion under the action of a manually operable control cam, and a notching lever handle manually operable to turn the ratchet wheel and cylindrical nut in an intermittent fashion through the intermediary of either one of the ratchet pawls as placed in mesh with the ratchet wheel.