Patents by Inventor Hirokazu Sugihara

Hirokazu Sugihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20080050765
    Abstract: Immobilization of malate dehydrogenase on a substrate using a glycerol solution containing malate dehydrogenase is achieved through dropping a mixed solution obtained by adding at least one selected from malic acid and malate to the glycerol containing malate dehydrogenase on the substrate, and drying it thereon. It is preferable to prepare the mixed solution by adding the malate to the glycerol solution containing malate dehydrogenase. The malate is preferably at least one selected from potassium malate and sodium malate.
    Type: Application
    Filed: October 30, 2007
    Publication date: February 28, 2008
    Applicant: MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.
    Inventors: Hidenobu Yaku, Hirokazu Sugihara
  • Patent number: 7326384
    Abstract: A device for detecting a physicochemical change in a biological sample comprising at least one measuring electrode provided by causing a conductive material to enter into a porous film, and a conductor connected to the measuring electrode.
    Type: Grant
    Filed: July 29, 2002
    Date of Patent: February 5, 2008
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara
  • Publication number: 20070134651
    Abstract: The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.
    Type: Application
    Filed: January 12, 2007
    Publication date: June 14, 2007
    Applicant: MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.
    Inventors: Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara
  • Patent number: 7172860
    Abstract: The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.
    Type: Grant
    Filed: October 6, 2003
    Date of Patent: February 6, 2007
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara
  • Patent number: 7041492
    Abstract: A multiple electrode includes a plurality of micro-electrodes provided on a substrate, and a wiring portion for providing an electrical signal to the micro-electrodes or extracting an electrical signal from the micro-electrodes. Each micro-electrode has porous conductive material on its surface, and the impedance of the micro-electrode is 50 k? or less. Preferably, the porous conductive material is gold, and formed by the passage of current at a current density of 1.0 to 5.0 A/dm2 for 10 to 360 sec. The multiple electrode may include micro-electrodes provided on a substrate in the form of a matrix, a lead line connected to the micro-electrodes, and an electrical junction connected to an end of the lead line.
    Type: Grant
    Filed: June 20, 2001
    Date of Patent: May 9, 2006
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Hiroaki Oka, Tetsuo Yukimasa, Ryuta Ogawa, Hirokazu Sugihara, Katsuyuki Tsuji, Yukifumi Yoshimoto
  • Publication number: 20060089489
    Abstract: A peptide nanofiber having conductivity is provided. A conductive peptide nanofiber which includes a nanofiber formed through a manner of self-assembly of a peptide that has a nanofiber-forming ability and consists of an amino acid sequence of Xaa-Phe-Ile-Val-Ile-Phe-Xaa (SEQ ID NO: 1, wherein N-terminal Xaa is an arbitrary amino acid residue Xaa1; C-terminal Xaa is an arbitrary amino acid residue Xaa2; and Xaa1 and Xaa2 are an amino acid having an acidic side chain, an amino acid having a basic side chain, or an amino acid having a side chain with polarity according as acidity and basicity) or a derivative of the peptide and a conductive substance added thereto, the aforementioned conductive substance being added to an amino group of the peptide or the derivative.
    Type: Application
    Filed: October 19, 2005
    Publication date: April 27, 2006
    Inventors: Kentaro Onizuka, Shuhei Tanaka, Hirokazu Sugihara, Atsuo Tamura
  • Patent number: 7006929
    Abstract: A sensor capable of simply and quickly measuring the electrical activities of a number of cells simultaneously and a device comprising the sensor and capable of detecting the signal transmission between subject cells are provided. The sensor comprises a plurality of electrodes provided on a substrate. The electrodes individually measure a signal generated by a biological sample held by each of the electrodes. Each of the electrodes comprises a depression for holding the biological sample, a throughhole which is in communication with the depression and penetrates to a rear side of the substrate, an electrode portion, and a drawing line from the electrode portion. The electrodes are arranged so that the samples held by the depressions electrically communicate with one another.
    Type: Grant
    Filed: June 5, 2002
    Date of Patent: February 28, 2006
    Assignees: Matsushita Electric Industrial Co., Ltd., The Ritsumeikan Trust
    Inventors: Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara, Satoshi Konishi
  • Patent number: 6999905
    Abstract: A method for extracting a signal of interest from a signal generated from at least one subject, comprises the steps of (A) obtaining a time-series signal generated from the subject as a data group consisting of a plurality of pieces of data, (B) obtaining a plurality of extracted data groups from the data group, wherein each extracted data group comprises a predetermined number of pieces of data selected from the data group, (C) calculating standard deviations of the plurality of extracted data groups to obtain a standard deviation group, and (D) referencing each standard deviation included in the standard deviation group and selecting the signal of interest.
    Type: Grant
    Filed: April 26, 2002
    Date of Patent: February 14, 2006
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Ryuta Ogawa, Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara
  • Patent number: 6894511
    Abstract: A multiple electrode for measuring electro-physiological characteristics of a biological specimen includes a plurality of micro-electrode provided on a first region on a substrate, and a reference electrode provided in a second region on the substrate. The reference electrode includes at least one stimulus reference electrode for applying an electrical signal to the plurality of micro-electrodes. Preferably, the reference electrode includes at least one measurement reference electrode for detecting an electrical signal from the plurality of micro-electrodes, and the stimulus reference electrode is electrically insulated from the measurement reference electrode. Preferably, the second region is placed at a distance from an outer edge of the first region, and surrounds the first region.
    Type: Grant
    Filed: July 12, 2001
    Date of Patent: May 17, 2005
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Tetsuo Yukimasa, Hiroaki Oka, Ryuta Ogawa, Hirokazu Sugihara
  • Patent number: 6890762
    Abstract: A method of measuring the physical and chemical properties of tissue or cells and a device for the same is provided, with which the physical and chemical environment of the tissue or cells can be changed arbitrarily corresponding to experimental necessities. The device comprises a system 40 for keeping the physical and chemical environment surrounding the biological tissue or cells constant, a system 50 for arbitrarily changing the physical and chemical environment, observation systems 10 and 20 for observing the physical and chemical properties of the tissue or cells, and a system 30 for comparing the change of the physical and chemical properties of the tissue or cells before and after changing the physical and chemical environment. The observation system 10 is a potential measurement device for measuring the electrophysiological properties of the tissue or cells.
    Type: Grant
    Filed: January 24, 1997
    Date of Patent: May 10, 2005
    Assignee: Matsushita Technical Information Services Co., Ltd.
    Inventors: Hirokazu Sugihara, Yasushi Kobayashi, Hiroaki Oka, Ryuta Ogawa, Makoto Taketani
  • Publication number: 20050004442
    Abstract: A measurement device 10 includes a measurement cell 11. The measurement cell 11 includes a substrates 17 and a substrate 16 formed on the substrate 17. The substrates 16 and 17 are bonded to each other. Thus, a flow pass 9 is formed in the measurement cell 11. As shown in FIG. 3, on the substrate 17 of the measurement cell 11, provided are a concave well 19, a protruding microelectrode 1 connected to a terminal 4a and formed on a side wall surface of the well 19, communicating tubes 15a and 15b connected to both of side surfaces of the well 19 (i.e., the flow pass 9) to face to each other, resistance baths 6a and 6b connected to the communicating tubes 15a and 15b, thin film resistors 3a and 3b arranged in the resistance baths 6a and 6b, and a reference electrode 2 connected to a terminal 4b.
    Type: Application
    Filed: November 19, 2002
    Publication date: January 6, 2005
    Inventors: Nobuhiko Ozaki, Hiroaki Oka, Hirokazu Sugihara
  • Publication number: 20040167723
    Abstract: A sensor capable of simply and quickly measuring the electrical activities of a number of cells simultaneously and a device comprising the sensor and capable of detecting the signal transmission between subject cells are provided. The sensor comprises a plurality of electrodes provided on a substrate. The electrodes individually measure a signal generated by a biological sample held by each of the electrodes. Each of the electrodes comprises a depression for holding the biological sample, a throughhole which is in communication with the depression and penetrates to a rear side of the substrate, an electrode portion, and a drawing line from the electrode portion. The electrodes are arranged so that the samples held by the depressions electrically communicate with one another.
    Type: Application
    Filed: December 5, 2003
    Publication date: August 26, 2004
    Inventors: Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara, Satoshi Konishi
  • Publication number: 20040137515
    Abstract: This invention relates to methods for the detection and characterization of psychoactives in neuronal tissue by comparing parameters measured for a set of baseline oscillations with those same parameters measured for a set of resulting oscillations. The baseline oscillations are induced or stimulated by such agents as chemical compounds that effect cholinergic interactions, co-deposited neuronal tissue, or electrical stimulations. The resulting set of oscillations is obtained after exposing the neuronal tissue to a sample composition that may or may not contain a psychoactive. By comparing the parameters for the baseline and resulting oscillations, the psychoactive in the sample composition may be detected and characterized as belonging to a specific psychoactive compound class and in some instances, may be distinguished from other members of the psychoactive compound class.
    Type: Application
    Filed: January 27, 2003
    Publication date: July 15, 2004
    Inventors: Gary Lynch, Makoto Taketani, Ken Shimono, Hirokazu Sugihara, Laura Colgin, Yousheng Jia
  • Publication number: 20040106139
    Abstract: The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1).
    Type: Application
    Filed: October 6, 2003
    Publication date: June 3, 2004
    Applicant: Matsushita Electric Industrial Co., LTD
    Inventors: Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara
  • Publication number: 20040076994
    Abstract: The method for detecting a target nucleic acid includes the steps of: preparing a nucleic acid probe labeled with a donor fluorescent substance and an acceptor substance placed to allow occurrence of fluorescence energy transfer, the nucleic acid probe having a sequence complementary to at least part of the target nucleic acid; mixing a sample solution with the nucleic acid probe to hybridize the target nucleic acid and the nucleic acid probe; and after the step of mixing, allowing the sample solution to act with sequence-independent nuclease and detecting the target nucleic acid from a change in the fluorescence intensity of the sample solution. This enables both enhancement of the degree of freedom in the design of the nucleic acid probe and simplification of the operation.
    Type: Application
    Filed: August 27, 2003
    Publication date: April 22, 2004
    Inventors: Hidenobu Yaku, Tetsuo Yukimasa, Hirokazu Sugihara
  • Publication number: 20030125887
    Abstract: A method for extracting a signal of interest from a signal generated from at least one subject, comprises the steps of (A) obtaining a time-series signal generated from the subject as a data group consisting of a plurality of pieces of data, (B) obtaining a plurality of extracted data groups from the data group, wherein each extracted data group comprises a predetermined number of pieces of data selected from the data group, (C) calculating standard deviations of the plurality of extracted data groups to obtain a standard deviation group, and (D) referencing each standard deviation included in the standard deviation group and selecting the signal of interest.
    Type: Application
    Filed: November 20, 2002
    Publication date: July 3, 2003
    Inventors: Ryuta Ogawa, Hiroaki Oka, Nobuhiko Ozaki, Hirokazu Sugihara
  • Publication number: 20030111343
    Abstract: A multiple electrode includes a plurality of micro-electrodes provided on a substrate, and a wiring portion for providing an electrical signal to the micro-electrodes or extracting an electrical signal from the micro-electrodes. Each micro-electrode has porous conductive material on its surface, and the impedance of the micro-electrode is 50 k&OHgr; or less. Preferably, the porous conductive material is gold, and formed by the passage of current at a current density of 1.0 to 5.0 A/dm2 for 10 to 360 sec. The multiple electrode may include micro-electrodes provided on a substrate in the form of a matrix, a lead line connected to the micro-electrodes, and an electrical junction connected to an end of the lead line.
    Type: Application
    Filed: February 1, 2002
    Publication date: June 19, 2003
    Inventors: Hiroaki Oka, Tetsuo Yukimasa, Ryuta Ogawa, Hirokazu Sugihara, Katsuyuki Tsuji, Yukifumi Yoshimoto
  • Publication number: 20030113833
    Abstract: An extracellular potential measuring device capable of simply and highly reliably detecting a change in a minute electrical signal emitted by a biological sample, and an extracellular potential measuring method using the device, and an apparatus comprising the device, are provided. The extracellular potential measuring device measures an extracellular potential with great precision and a high output. The device comprises at least one well comprising means for holding a cell provided on a substrate, a measuring electrode for detecting an electrical signal of each of the at least one well, and a reference electrode. The cell holding means comprises at least one depression provided within the well, and has a throughhole at a bottom surface thereof, the throughhole being linked to means for suctioning the cell.
    Type: Application
    Filed: November 4, 2002
    Publication date: June 19, 2003
    Inventors: Hiroaki Oka, Ryuta Ogawa, Tetsuo Yukimasa, Hirokazu Sugihara, Satoshi Konishi, Hideyasu Jikoh, Nobuhiko Ozaki, Fumiaki Emoto
  • Patent number: RE38323
    Abstract: A cell potential measurement apparatus, which uses a planar electrode enabling a multi-point simultaneous measurement of potential change arising from cell activities, is provided which can conduct measurements accurately and efficiently as well as can improve convenience of arranging measurement results.
    Type: Grant
    Filed: October 8, 1998
    Date of Patent: November 18, 2003
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Hirokazu Sugihara, Akihito Kamei, Yasushi Kobayashi, Makoto Taketani, Tadayasu Mitsumata
  • Patent number: RE40209
    Abstract: A cell potential measurement apparatus, which uses a planar electrode enabling a multi-point simultaneous measurement of potential change arising from cell activities, is provided which can conduct measurements accurately and efficiently as well as can improve convenience of arranging measurement results.
    Type: Grant
    Filed: October 13, 2000
    Date of Patent: April 1, 2008
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Hirokazu Sugihara, Akihito Kamei, Yasushi Kobayashi, Makoto Taketani, Tadayasu Mitsumata