Abstract: A mixing container includes a first container that stores two or more reagents and a second container for mixing two or more reagents. The first container includes reagent storage chambers that are respectively provided for the two or more reagents and have openings formed at portions thereof, and a sealing member that seals the respective openings of the reagent storage chambers. The second container includes an opening portion formed such that the first container can be connected thereto, and protruding portions disposed so as to pierce the sealing member when the first container is connected to the opening portion.

Abstract: A mixing container includes a first container that stores two or more reagents and a second container for mixing two or more reagents. The first container includes reagent storage chambers that are respectively provided for the two or more reagents and have openings formed at portions thereof, and a sealing member that seals the respective openings of the reagent storage chambers. The second container includes an opening portion formed such that the first container can be connected thereto, and protruding portions disposed so as to pierce the sealing member when the first container is connected to the opening portion.

Abstract: Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently.

Abstract: Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently. In the method, a specimen analysis tool containing substances that specifically bind to a target component contained in a sample is used. The specimen analysis tool is obtained by arranging a sample supplying portion, a reagent portion, and a detection portion on the porous base material from upstream to downstream in a sample moving direction in this order. The reagent portion contains a labeled substance that specifically binds to the target component. The detection portion contains an immobilized substance that specifically binds to the target component.

Abstract: The present inventors worked on elucidating the mechanism of antigen cross-presentation by dendritic cells, and revealed that foreign antigens taken up in dendritic cells are degraded by proteasomes after undergoing polyubiquitination. Based on the novel finding that polyubiquitination is involved in cross-presentation, the promotion of polyubiquitination was attempted by a number of methods, and the promotion of antigen presentation was confirmed. These methods enable the production of dendritic cells effective for inducing CTL activation.

Abstract: The present inventors worked on elucidating the mechanism of antigen cross-presentation by dendritic cells, and revealed that foreign antigens taken up in dendritic cells are degraded by proteasomes after undergoing polyubiquitination. They discovered a positive correlation between the uptake amount of an antigenic protein by dendritic cells or the level of polyubiquitination and the level of antigen presentation of the antigenic protein. Based on these findings, methods were developed for predicting the intensity of cross-presentation of an anti genic protein by measuring the amount of antigenic protein taken up or polyubiquitinated protein. The use of these methods allows selecting antigenic proteins that are readily presented as antigens from among a number of proteins. The methods of the present invention enable suitable cancer immune vaccines to be provided through selection of more suitable cancer-specific proteins.