Patents by Inventor Hiroshi Ezura

Hiroshi Ezura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200318125
    Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.
    Type: Application
    Filed: January 19, 2017
    Publication date: October 8, 2020
    Applicant: RIKEN
    Inventors: Keiji NUMATA, Yoko HORII, Takeshi YOSHIZUMI, Taku DEMURA, Yutaka KODAMA, Takashi YAMAMOTO, Tetsushi SAKUMA, Kenji MIURA, Hiroshi EZURA
  • Patent number: 10745710
    Abstract: The present invention relates to a plant having an improved sugar content in fruit compared with its wild type plant, which has a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein. The present invention also relates to a parthenocarpic plant having a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: August 18, 2020
    Assignee: UNIVERSITY OF TSUKUBA
    Inventors: Hiroshi Ezura, Tohru Ariizumi, Jun-ichiro Masuda, Yoshihiro Okabe
  • Patent number: 10385357
    Abstract: Provided is a plant such as tomato, in which plant vigor has been increased and heat resistance has further been imparted. A plant such as tomato can be obtained, which has increased the stem diameter as well as the plant height and has become heat resistant by having a mutant Della protein, in which a leucine corresponding to the leucine at position 567 in SEQ ID NO: 2 in the amino acid sequence of the Della protein has been replaced by another amino acid, preferably phenylalanine.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: August 20, 2019
    Assignees: UNIVERSITY OF TSUKUBA, RIKEN
    Inventors: Hiroshi Ezura, Tohru Ariizumi, Yoshihito Shinozaki, Junji Kimbara, Miyako Kusano, Atsushi Fukushima, Kazuki Saito
  • Patent number: 10349611
    Abstract: A heat-tolerant tomato plant exhibiting a high capacity for developing seed-containing fruits under high temperature conditions is provided. The present invention relates to a method for producing a heat-tolerant tomato plant comprising introducing a genetic mutation into a tomato plant, wherein the mutation improves the pollen viability and the capacity for developing seed-containing fruits under high temperature conditions compared with wild-type plant; and a heat-tolerant tomato plant into which the mutation has been introduced.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: July 16, 2019
    Assignee: UNIVERSITY OF TSUKUBA
    Inventors: Hiroshi Ezura, Ken Hoshikawa, Shoma Fukumoto, Sayaka Ooshima, Mina Aiba
  • Publication number: 20190110420
    Abstract: The present invention relates to a plant having an improved sugar content in fruit compared with its wild type plant, which has a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein. The present invention also relates to a parthenocarpic plant having a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein.
    Type: Application
    Filed: August 5, 2016
    Publication date: April 18, 2019
    Inventors: Hiroshi EZURA, Tohru ARIIZUMI, Jun-ichiro MASUDA, Yoshihiro OKABE
  • Patent number: 9944936
    Abstract: The present invention relates to an Agrobacterium having improved gene transfer efficiency. The present invention provides a transformed Agrobacterium which harbors a foreign GABA transaminase gene and exhibits improved gene transfer efficiency, and a method for producing a transformed plant using the same.
    Type: Grant
    Filed: February 7, 2014
    Date of Patent: April 17, 2018
    Assignee: UNIVERSITY OF TSUKUBA
    Inventors: Hiroshi Ezura, Koji Nakamura, Satoko Nonaka, Tatsuhiko Someya, Sha Zhou
  • Publication number: 20170292130
    Abstract: A heat-tolerant tomato plant exhibiting a high capacity for developing seed-containing fruits under high temperature conditions is provided. The present invention relates to a method for producing a heat-tolerant tomato plant comprising introducing a genetic mutation into a tomato plant, wherein the mutation improves the pollen viability and the capacity for developing seed-containing fruits under high temperature conditions compared with wild-type plant; and a heat-tolerant tomato plant into which the mutation has been introduced.
    Type: Application
    Filed: September 25, 2015
    Publication date: October 12, 2017
    Applicant: UNIVERSITY OF TSUKUBA
    Inventors: Hiroshi EZURA, Ken HOSHIKAWA, Shoma FUKUMOTO, Sayaka OOSHIMA, Mina AIBA
  • Publication number: 20160264989
    Abstract: Provided is a plant such as tomato, in which plant vigor has been increased and heat resistance has further been imparted. A plant such as tomato can be obtained, which has increased the stem diameter as well as the plant height and has become heat resistant by having a mutant Della protein, in which a leucine corresponding to the leucine at position 567 in SEQ ID NO: 2 in the amino acid sequence of the Della protein has been replaced by another amino acid, preferably phenylalanine.
    Type: Application
    Filed: November 7, 2014
    Publication date: September 15, 2016
    Inventors: Hiroshi EZURA, Tohru ARIIZUMI, Yoshihito SHINOZAKI, Junji KIMBARA, Miyako KUSANO, Atsushi FUKUSHIMA, Kazuki SAITO
  • Publication number: 20150368658
    Abstract: The present invention relates to an Agrobacterium having improved gene transfer efficiency. The present invention provides a transformed Agrobacterium which harbors a foreign GABA transaminase gene and exhibits improved gene transfer efficiency, and a method for producing a transformed plant using the same.
    Type: Application
    Filed: February 7, 2014
    Publication date: December 24, 2015
    Applicant: UNIVERSITY OF TSUKUBA
    Inventors: Hiroshi EZURA, Koji NAKAMURA, Satoko NONAKA, Tatsuhiko SOMEYA, Sha ZHOU
  • Publication number: 20140020134
    Abstract: The present invention relates to a fruit-specific promoter, which is suitable for the expression in a broader range of developmental stages of a fruit. Provided is a fruit-specific promoter DNA, which consists of a nucleotide sequence having 85% or more identity with the nucleotide sequence as shown in SEQ ID NO: 1 or 2 and has promoter activity in mature-green fruits.
    Type: Application
    Filed: March 30, 2011
    Publication date: January 16, 2014
    Applicants: INPLANTA INNOVATIONS INC., UNIVERSITY OF TSUKUBA, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Nobuyo Itou, Hirofumi Kuroda, Ken-ichi Takane, Kou Aoki, Daisuke Shibata, Hiroshi Ezura, Kyoko Tanase
  • Publication number: 20090205068
    Abstract: The present invention relates to: a method for producing a transgenic plant expressing miraculin, comprising introducing a miraculin gene into a useful plant that can be easily produced and cultivated throughout the year using plant molecular breeding technology; a transgenic plant expressing miraculin, which is produced by the method; and the use thereof. The present invention also relates to a food additive, a food or drink, or an antidiabetic drug, which contains miraculin that is produced by the transgenic plant.
    Type: Application
    Filed: August 4, 2005
    Publication date: August 13, 2009
    Applicants: Hiroshi EZURA, INPLANTA INNOVATIONS INC
    Inventors: Hiroshi Ezura, Hyeon-Jin Sun, Masanobu Kirita, Takanari Ichikawa, Nobuyo Nishizaki