Patents by Inventor Hirotsugu Kubo

Hirotsugu Kubo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200291350
    Abstract: The presently disclosed subject matter is to provide a hepatocyte structure, particularly, a hepatocyte structure that mimics nonalcoholic steatohepatitis (NASH). A hepatocyte construct including an aggregate containing hepatocytes and adherent cells that are non-hepatocytes, and wherein the hepatocytes include ballooned hepatocytes is provided. Further, a method for producing a hepatocyte construct, comprising: (i) forming an aggregate by aggregating a cell group comprising hepatocytes and adherent cells that are non-hepatocytes; and (ii) culturing the aggregate is provided.
    Type: Application
    Filed: July 3, 2019
    Publication date: September 17, 2020
    Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Botao GAO, Yuki KAGAWA, Tetsuya OGAWA, Hirotsugu KUBO, Tatsuya SHIMIZU, Katsuhisa SAKAGUCHI
  • Publication number: 20200278342
    Abstract: A device for measuring a tension of a cell structure containing muscle cells includes a first and a second gel adaptor holder. The first gel adaptor holder includes a frame member and a first gel holding portion which is disposed protrudingly from a part of an inner surface of the frame member for fixing one end of a gel. The second gel adaptor holder includes a second gel holding portion that fixes another end of the gel, and connecting members connected with the second gel holding portion. A kit including the device, a substrate and a gel forming cover. The substrate includes a pair of gel shaping convex parts fitted along the inner surface of the frame member. The gel forming cover includes a surface parallel to a gel contacting surface of the substrate, in order to form an upper surface of the gel.
    Type: Application
    Filed: February 27, 2020
    Publication date: September 3, 2020
    Applicant: NIHON KOHDEN CORPORATION
    Inventors: Hirotsugu Kubo, Takahiro Shioyama, Yuki Kagawa, Yuto Hinata, Hodaka Makino
  • Publication number: 20200140807
    Abstract: The present invention provides a method for swiftly producing a layered cell sheet that is non-invasively obtained and is utilizable for transplantation, etc., the method including (1) a step of applying a centrifugal force to a first cell sheet on a temperature-responsive culture surface for a predetermined time in a temperature range from a lower critical solution temperature of the temperature-responsive culture surface to 45° C., (2) a step of further placing a second cell sheet on the first cell sheet, and (3) a step of applying a centrifugal force to the first cell sheet and the second cell sheet on the temperature-responsive culture surface for a predetermined time in the temperature range from the lower critical solution temperature to 45° C.; and also provides a layered cell sheet obtained by the method.
    Type: Application
    Filed: June 28, 2018
    Publication date: May 7, 2020
    Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Yuki KAGAWA, Tatsuya SHIMIZU, Yuji HARAGUCHI, Hirotsugu KUBO
  • Publication number: 20200123489
    Abstract: A sample storage apparatus includes: a lid member; a sample storage container; first and second fluid sucking sections; first and second fluid discharging sections; first to fourth flow paths; and a first fluid identification sensor. The first flow path is connected to the first fluid sucking section, the second flow path is connected to the second fluid sucking section, the third flow path is connected to the first fluid discharging section, and the fourth flow path is connected to the second fluid discharging section. The first fluid identification sensor for identifying a fluid is disposed in the first flow path.
    Type: Application
    Filed: October 22, 2019
    Publication date: April 23, 2020
    Applicants: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITY
    Inventors: Hodaka MAKINO, Hirotsugu KUBO, Tetsuya OGAWA, Masahiro KINOOKA
  • Publication number: 20190247842
    Abstract: An aseptic sampling apparatus includes an isolator, a liquid delivery port that is disposed in the isolator, a sampling section that is disposed inside the isolator, a first flow path that communicates with a discharge flow path of the sampling section, and that connects an inside and outside of the isolator to each other through the liquid delivery port, a fluid supplying unit that supplies a fluid to the sampling section, a gas supplying unit that communicates with the fluid supplying unit, and a seal member that prevents the fluid supplied from fluid supplying unit to the discharge flow path from leaking.
    Type: Application
    Filed: January 3, 2019
    Publication date: August 15, 2019
    Applicants: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITY
    Inventors: Hodaka MAKINO, Hirotsugu KUBO, Tetsuya OGAWA, Masahiro KINOOKA
  • Publication number: 20190250073
    Abstract: An aseptic sampling flow path kit to be applied to an isolator having a liquid delivery port includes a sampling section, a first flow path that communicates with the sampling section, and that connects an inside of the isolator to an outside of the isolator through the liquid delivery port, and at least one one-way valve which is disposed in the first flow path, and that limits flow of fluid in the first flow path to a direction from the sampling section toward the liquid delivery port. In the kit, at least a part of the first flow path is a germicidal flow path to which a germicidal unit is applicable.
    Type: Application
    Filed: January 2, 2019
    Publication date: August 15, 2019
    Applicants: NIHON KOHDEN CORPORATION, OSAKA UNIVERSITY
    Inventors: Hodaka MAKINO, Hirotsugu KUBO, Masahiro KINOOKA
  • Patent number: 10365264
    Abstract: A measuring apparatus relates to a contractile ability of a spontaneously pulsating myocardial tissue. The measuring apparatus includes a measurement table and a pressure sensor. The measurement table includes a storage section having an opening and configured to hold liquid inside the storage section, and an attaching portion on which the myocardial tissue is attached around the opening. The pressure sensor is disposed inside the storage section.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: July 30, 2019
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Hirotsugu Kubo, Tatsuya Shimizu, Tetsutaro Kikuchi
  • Publication number: 20190120742
    Abstract: A device for measuring a tension of a sheet-like tissue containing cardiomyocytes includes a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member for fixing one end of a film-like gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member. A kit includes the tension measuring device; a substrate having a pair of gel molding protruding members fitted along the inside face of the frame member; and a gel forming lid body having a face parallel to a gel contact face of the substrate so as to form an upper face of the gel. Further, a system for measuring the tension includes the tension measuring device.
    Type: Application
    Filed: October 24, 2018
    Publication date: April 25, 2019
    Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Hirotsugu KUBO, Takahiro SHIOYAMA, Yuki KAGAWA, Tatsuya SHIMIZU, Daisuke SASAKI
  • Publication number: 20180067098
    Abstract: A measuring apparatus relates to a contractile ability of a spontaneously pulsating myocardial tissue. The measuring apparatus includes a measurement table and a pressure sensor. The measurement table includes a storage section having an opening and configured to hold liquid inside the storage section, and an attaching portion on which the myocardial tissue is attached around the opening. The pressure sensor is disposed inside the storage section.
    Type: Application
    Filed: August 31, 2017
    Publication date: March 8, 2018
    Inventors: Hirotsugu KUBO, Tatsuya SHIMIZU, Tetsutaro KIKUCHI
  • Patent number: 9783773
    Abstract: A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.
    Type: Grant
    Filed: March 28, 2012
    Date of Patent: October 10, 2017
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Mitsuhiro Oura, Teruo Okano, Tatsuya Shimizu, Hirotsugu Kubo, Sunao Takeda
  • Patent number: 9708578
    Abstract: A measuring unit which is to be attached to a cell culture container configured to accommodate cells to be cultured and a culture solution, includes: a measurer which is configured to measure information related to the cells and the culture solution, in a non-contact manner; and a sensor which is configured to detect at least one of a position, a posture, an impact, an orientation, and vibration.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: July 18, 2017
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Mitsuhiro Oura, Hirotsugu Kubo, Katsuyoshi Suzuki, Katsuhide Tone, Teruo Okano, Tatsuya Shimizu
  • Patent number: 9658214
    Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.
    Type: Grant
    Filed: July 19, 2013
    Date of Patent: May 23, 2017
    Assignee: NIHON KOHDEN CORPORATION
    Inventors: Takahiro Shioyama, Akane Suzuki, Hirotsugu Kubo, Sunao Takeda
  • Patent number: 9470572
    Abstract: A method of measuring a liquid level of a cell culture solution stored in a cell vessel, includes: irradiating the cell culture solution with at least two kinds of light beams including a first light beam having a first wavelength and a second light beam having a second wavelength; measuring a first absorbance of the cell culture solution with respect to the first light beam, and a second absorbance of the cell culture solution with respect to the second light beam; and determining a liquid level of the cell culture solution based on the first absorbance and the second absorbance.
    Type: Grant
    Filed: March 21, 2013
    Date of Patent: October 18, 2016
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Mitsuhiro Oura, Hirotsugu Kubo, Teruo Okano, Tatsuya Shimizu
  • Patent number: 9443127
    Abstract: A cell analyzing apparatus includes: a first histogram acquiring section which is configured to acquire a first histogram of fluorescence intensities by using a result of a measurement of a number of nuclear stained cells; a second histogram acquiring section which is configured to acquire a second histogram that is normalized based on a fluorescence intensity value indicating a maximum value of the first histogram; a determining section which is configured to determine whether cancer cells exist or not, based on one of the first histogram and the second histogram; and an outputting section which is configured to output a result of the determination performed by the determining section.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: September 13, 2016
    Assignee: Nihon Kohden Corporation
    Inventors: Sunao Takeda, Hirotsugu Kubo, Takahiro Shioyama, Akane Suzuki, Kenichi Nomura, Yo Kato, Nae Hinata
  • Patent number: 9304088
    Abstract: A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.
    Type: Grant
    Filed: February 19, 2012
    Date of Patent: April 5, 2016
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Mitsuhiro Oura, Teruo Okano, Tatsuya Shimizu, Hirotsugu Kubo, Sunao Takeda, Shinji Yamamori
  • Patent number: 9291572
    Abstract: A pH measuring method includes: illuminating a medium solution, which includes: a first material; and a second material, with a plurality of light beams, which includes: a first light beam, a wavelength of which corresponds to a first absorption peak; a second light beam, a wavelength of which corresponds to a second absorption peak or a second convergence point; a third light beam, a wavelength of which corresponds to a third absorption peak or a third convergence point; and a fourth light beam, a wavelength of the fourth light beam at which an absorbance of at least one of the first and second materials is converged irrespective of a pH; receiving transmitted or reflected light beams of the first to fourth light beams; measuring absorbances at the wavelengths of the received light beams respectively; and calculating a pH of the medium solution based on the absorbances.
    Type: Grant
    Filed: April 6, 2012
    Date of Patent: March 22, 2016
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Mitsuhiro Oura, Hirotsugu Kubo, Sunao Takeda, Teruo Okano, Tatsuya Shimizu
  • Publication number: 20150078649
    Abstract: A cell analyzing apparatus includes: a first histogram acquiring section which is configured to acquire a first histogram of fluorescence intensities by using a result of a measurement of a number of nuclear stained cells; a second histogram acquiring section which is configured to acquire a second histogram that is normalized based on a fluorescence intensity value indicating a maximum value of the first histogram; a determining section which is configured to determine whether cancer cells exist or not, based on one of the first histogram and the second histogram; and an outputting section which is configured to output a result of the determination performed by the determining section.
    Type: Application
    Filed: September 17, 2014
    Publication date: March 19, 2015
    Applicant: Nihon Kohden Corporation
    Inventors: Sunao TAKEDA, Hirotsugu KUBO, Takahiro SHIOYAMA, Akane SUZUKI, Kenichi NOMURA, Yo KATO, Nae HINATA
  • Publication number: 20140030728
    Abstract: A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue.
    Type: Application
    Filed: July 19, 2013
    Publication date: January 30, 2014
    Applicant: NIHON KOHDEN CORPORATION
    Inventors: Takahiro Shioyama, Akane Suzuki, Hirotsugu Kubo, Sunao Takeda
  • Publication number: 20130330248
    Abstract: A cell isolation apparatus in which tissue and fluid that are accommodated in a container are pipetted by a pipette, and cells are isolated from the tissue, the cell isolation apparatus includes: a nozzle to which the pipette is attached; a pump which is connected to the nozzle; a controller which is configured to control an operation of the pump to cause air to be ejected from the nozzle or to be sucked into the nozzle, thereby causing the pipette to perform pipetting; and a condition inputting unit in which a user inputs a condition of control of the controller.
    Type: Application
    Filed: May 29, 2013
    Publication date: December 12, 2013
    Applicant: NIHON KOHDEN CORPORATION
    Inventors: Takahiro SHIOYAMA, Akane SUZUKI, Hirotsugu KUBO
  • Publication number: 20130255374
    Abstract: A method of measuring a liquid level of a cell culture solution stored in a cell vessel, includes: irradiating the cell culture solution with at least two kinds of light beams including a first light beam having a first wavelength and a second light beam having a second wavelength; measuring a first absorbance of the cell culture solution with respect to the first light beam, and a second absorbance of the cell culture solution with respect to the second light beam; and determining a liquid level of the cell culture solution based on the first absorbance and the second absorbance.
    Type: Application
    Filed: March 21, 2013
    Publication date: October 3, 2013
    Applicants: TOKYO WOMEN'S MEDICAL UNIVERSITY, NIHON KOHDEN CORPORATION
    Inventors: Mitsuhiro Oura, Hirotsugu Kubo, Teruo Okano, Tatsuya Shimizu