Patents by Inventor Hiroyuki Kanamaru

Hiroyuki Kanamaru has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10435732
    Abstract: The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.
    Type: Grant
    Filed: June 9, 2015
    Date of Patent: October 8, 2019
    Assignee: KANEKA CORPORATION
    Inventors: Hiroyuki Kanamaru, Teruaki Takesue, Osamu Odawara, Takeyuki Tsuchidate
  • Publication number: 20170211116
    Abstract: The present invention provides a method for producing a recombinant protein, which can increase the cell growth rate in culture while stably maintaining plasmids in the cells, thereby improving the recombinant protein yield. The present invention provides a method for producing a recombinant protein, including a high-temperature culture step of culturing at 32° C. or higher a Brevibacillus bacterium that contains a gene encoding a recombinant protein, and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32° C. after the high-temperature culture step.
    Type: Application
    Filed: June 9, 2015
    Publication date: July 27, 2017
    Applicant: Kaneka Corporation
    Inventors: Hiroyuki KANAMARU, Teruaki TAKESUE, Osamu ODAWARA, Takeyuki TSUCHIDATE
  • Patent number: 9493762
    Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: November 15, 2016
    Assignee: KANEKA CORPORATION
    Inventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
  • Patent number: 9273332
    Abstract: The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
    Type: Grant
    Filed: October 11, 2007
    Date of Patent: March 1, 2016
    Assignee: Kaneka Corporation
    Inventors: Hiroyuki Kanamaru, Makoto Ueda, Hirokazu Nanba
  • Patent number: 9267116
    Abstract: The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.
    Type: Grant
    Filed: December 8, 2009
    Date of Patent: February 23, 2016
    Assignee: KANEKA CORPORATION
    Inventors: Hiroyuki Kanamaru, Ryuuji Miki, Makoto Ueda, Hironori Nanba, Yoshihiko Yasohara
  • Publication number: 20140335575
    Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.
    Type: Application
    Filed: September 28, 2012
    Publication date: November 13, 2014
    Inventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
  • Patent number: 8227228
    Abstract: The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
    Type: Grant
    Filed: August 2, 2006
    Date of Patent: July 24, 2012
    Assignee: Kaneka Corporation
    Inventors: Hiroyuki Kanamaru, Makoto Ueda, Ryuuji Miki, Hirokazu Nanba
  • Publication number: 20110281309
    Abstract: The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.
    Type: Application
    Filed: December 8, 2009
    Publication date: November 17, 2011
    Applicant: KANEKA CORPORATION
    Inventors: Hiroyuki Kanamaru, Ryuuji Miki, Makoto Ueda, Hironori Nanba, Yoshihiko Yasohara
  • Publication number: 20110086396
    Abstract: The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
    Type: Application
    Filed: August 2, 2006
    Publication date: April 14, 2011
    Applicant: Kaneka Corporation
    Inventors: Hiroyuki Kanamaru, Makoto Ueda, Ruuji Miki, Hirokazu Nanba
  • Publication number: 20100028959
    Abstract: The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
    Type: Application
    Filed: October 11, 2007
    Publication date: February 4, 2010
    Applicant: Kaneka Corporation
    Inventors: Hiroyuki Kanamaru, Makoto Ueda, Hirokazu Nanba
  • Patent number: 5244985
    Abstract: Disclosed are an epoxidized polyester which comprises, per molecule, at least three bifunctional groups each having the general formula ##STR1## wherein R.sup.1 and R.sup.2 are the same or different and each is a hydrogen atom or an alkyl group containing 1 to 5 carbon atoms, or R.sup.1 and R.sup.2 taken together represent an endomethylene group, and which has a number average molecular weight of about 1,000 to about 10,000, as well as processes for preparing the epoxidized polyester.
    Type: Grant
    Filed: March 25, 1992
    Date of Patent: September 14, 1993
    Assignee: New Japan Chemical Co., Ltd.
    Inventors: Tomio Nobe, Hiroyuki Kanamaru, Harutomo Nomoto, Shoji Tani
  • Patent number: 4983391
    Abstract: An insecticide for protecting a fibrous material from the damage by an insect, which comprises a carbamic ester of the formula: ##STR1## wherein R.sub.1 and R.sub.2 are, the same or different, each a hydrogen atom or a lower alkyl group and R.sub.3 is a lower alkyl group as an active ingredient.
    Type: Grant
    Filed: August 9, 1989
    Date of Patent: January 8, 1991
    Assignee: S.T. Chemical Co., Ltd.
    Inventors: Ryonosuke Muneyuki, Hiroyuki Kanamaru
  • Patent number: 4912137
    Abstract: An insecticide which comprises an oxalic ester of the formula: ##STR1## wherein R.sub.1 and R.sub.2 are, the same or different, each a hydrogen atom or a C.sub.1 -C.sub.4 alkyl group but at least one of them is a C.sub.1 -C.sub.4 alkyl group.
    Type: Grant
    Filed: July 12, 1988
    Date of Patent: March 27, 1990
    Assignee: S. T. Chemical Co., Ltd.
    Inventors: Ryonosuke Muneyuki, Hiroyuki Kanamaru