Patents by Inventor Hiroyuki Noji

Hiroyuki Noji has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200407769
    Abstract: Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).
    Type: Application
    Filed: March 4, 2019
    Publication date: December 31, 2020
    Inventors: Kazuhito Tabata, Yoshihiro Minagawa, Hiroyuki Noji
  • Patent number: 10809257
    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
    Type: Grant
    Filed: January 29, 2019
    Date of Patent: October 20, 2020
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki Noji, Ryota Iino, Suguru Araki
  • Publication number: 20200283256
    Abstract: A filament winding apparatus winds, around a workpiece, a fiber bundle formed by bundling a plurality of fibers. The filament winding apparatus includes a widening roller that rotates while making contact with the fiber bundle that is being conveyed. The widening roller has, provided on the peripheral surface, a plurality of projecting ridges extending in the axial direction, the projecting ridges being arranged side by side in the circumferential direction. The projecting ridges make contact with the fiber bundle to thereby widen the fiber bundle.
    Type: Application
    Filed: March 6, 2020
    Publication date: September 10, 2020
    Inventors: Yohei NOJI, Kosuke TATSUSHIMA, Hiroyuki TAKAHASHI, Kenta UMETSU
  • Publication number: 20200261877
    Abstract: A method for forming a lipid membrane vesicle includes: filling a chamber with a first aqueous solution by introducing it to a liquid flow path facing a microreactor chip hydrophobic layer main surface; forming a first lipid monolayer membrane in an opening part of the chamber filled with the solution; forming a second lipid monolayer membrane on a layer interface of the organic solvent formed on the main surface of the hydrophobic layer with a second aqueous solution by introducing the solution to the liquid flow path; allowing a first aqueous solution form in the chamber to alter to a spherical droplet covered with the first lipid monolayer membrane; and forming a lipid membrane vesicle by moving the droplet to a position of the second lipid monolayer membrane by applying a physical action, and by zipping the first lipid monolayer membrane covering the droplet and the second lipid monolayer membrane.
    Type: Application
    Filed: April 19, 2018
    Publication date: August 20, 2020
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Rikiya WATANABE, Naoki SOGA, Hiroyuki NOJI
  • Publication number: 20200173925
    Abstract: Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.
    Type: Application
    Filed: March 28, 2018
    Publication date: June 4, 2020
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki NOJI, Kazuhito TABATA
  • Publication number: 20200049704
    Abstract: Disclosed is an analyte detection method, including detecting an analyte in such a state that a complex composed of the analyte in a sample of interest, a particle capable of bonding to the analyte and a trapping substance capable of bonding to the analyte is formed on a substrate, wherein the analyte is detected on the basis of an index associated with a behavior of the particle.
    Type: Application
    Filed: August 9, 2019
    Publication date: February 13, 2020
    Applicant: SYSMEX CORPORATION
    Inventors: Kenji AKAMA, Hiroyuki NOJI
  • Publication number: 20200038860
    Abstract: A microreactor chip includes a substrate and a hydrophobic layer that is a layer provided on the substrate and made of a hydrophobic substance and is formed so that openings of a plurality of chambers are arranged regularly on a main surface of the layer. Each chamber is provided with a first lipid bilayer membrane and a second lipid bilayer membrane that are disposed with a gap therebetween in a depth direction so as to fractionate the chamber in the depth direction.
    Type: Application
    Filed: March 2, 2018
    Publication date: February 6, 2020
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Rikiya WATANABE, Hiroyuki NOJI, Naoki SOGA
  • Patent number: 10471429
    Abstract: A material film is formed as a thin film having a thickness of 1 ?m on a surface of a glass substrate. A plurality of micro-chambers having a diameter of 5 ?m are formed in the material film to be arrayed at a high density. The respective chambers filled with an aqueous test solution have openings that are liquid-sealed by a lipid bilayer membrane to provide a high-density micro-chamber array. Significant downsizing of the micro-chambers enhances a change in concentration by a reaction of one biomolecule in the chamber and thereby increases the detection sensitivity. In the configuration that a large number of micro-chambers are formed at a high density, even in the case of an extremely slow reaction of the biomolecule, the reaction proceeds in any of the chambers. This configuration accordingly enables the reaction of the biomolecule to be detected with high sensitivity.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: November 12, 2019
    Assignee: THE UNIVERSITY OF TOKYO
    Inventors: Hiroyuki Noji, Rikiya Watanabe, Hiroaki Suga, Daishi Fujita
  • Patent number: 10451619
    Abstract: As a technique for efficiently sealing many substances, such as beads, nucleic acid, protein, virus, cells, and lipid membrane complex, into an array, the present invention provides a method for sealing a substance, including: (i) a step of introducing a first solvent containing a substance on a substrate on which a plurality of receptacles capable of storing the substance are formed separated from each other by a side wall; and (ii) a step of introducing a second solvent having a greater specific gravity than that of the first solvent onto the first solvent, the step (ii) being carried out after the step (i).
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: October 22, 2019
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki Noji, Lisa Yamauchi
  • Publication number: 20190194717
    Abstract: Provided is a method for detecting pathogenic microorganisms in a biological sample, which is a technique that can be used to perform high-sensitivity detection of pathogenic microorganisms, such as influenza virus, etc.
    Type: Application
    Filed: September 4, 2017
    Publication date: June 27, 2019
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki NOJI, Kazuhito TABATA
  • Publication number: 20190154682
    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
    Type: Application
    Filed: January 29, 2019
    Publication date: May 23, 2019
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki NOJI, Ryota IINO, Suguru ARAKI
  • Patent number: 10267793
    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: April 23, 2019
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki Noji, Ryota Iino, Suguru Araki
  • Publication number: 20190085385
    Abstract: A method of detecting a target molecule including binding, to first target molecules, specific binding substances which are capable of recognizing different epitopes and labeled with nucleic acid fragments, respectively, detecting the specific binding substances based on fluorescence signals of different fluorescence wavelengths from the nucleic acid fragments, and detecting the first target molecules based on the fluorescence signals that correspond to the specific binding substances, respectively.
    Type: Application
    Filed: November 19, 2018
    Publication date: March 21, 2019
    Applicant: TOPPAN PRINTING CO., LTD.
    Inventors: Yoichi Makino, Takumi Hirase, Noriaki Arai, Hiroyuki Noji
  • Patent number: 10018627
    Abstract: A technique for efficiently sealing many substances, such as beads, nucleic acid, protein, virus, cells, and lipid membrane complex, into an array is provided. The present invention provides a method for sealing a substance, including: (i) a step of introducing a first solvent containing a substance on a substrate on which a plurality of receptacles capable of storing the substance are formed separated from each other by a side wall; and (ii) a step of introducing a second solvent having a greater specific gravity than that of the first solvent onto the first solvent, the step (ii) being carried out after the step (i).
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: July 10, 2018
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki Noji, Lisa Yamauchi
  • Publication number: 20180104686
    Abstract: A high-density micro-chamber array has a translucent flat substrate, a hydrophobic layer in which a plurality of micro-chambers are provided, and a lipid bilayer membrane formed in each of the openings of the micro-chambers, wherein an electrode is provided in each of the micro-chambers, and when the side of the substrate on which the hydrophobic layer is provided is directed upward, the micro-chamber array is configured such that with at least one of the following A) and B) being met, light entering the substrate from below is transmitted through the substrate and penetrates into the micro-chambers' interiors, and light entering the substrate from the micro-chambers' interiors is transmitted through the substrate and escapes toward below the substrate. A) The electrode is provided on an inner side surface of each of the micro-chambers. B) The electrode is transparent and provided on a bottom surface of each of the micro-chambers.
    Type: Application
    Filed: June 7, 2016
    Publication date: April 19, 2018
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Rikiya WATANABE, Hiroyuki NOJI, Naoki SOGA
  • Patent number: 9797837
    Abstract: There are provided a detector and a detection method capable of detecting a biological sample with high sensitivity. A detector is provided with a microchamber array including a plurality of storage sections to be filled with a hydrophilic solvent containing a biological sample; and an image sensor in which picture elements are disposed corresponding to the storage sections, wherein the microchamber array includes a flow channel in communication with openings of the storage sections; a hydrophobic solvent supply unit arranged in continuity with the flow channel; and a through-hole which allows the hydrophilic solvent to enter and exit the flow channel, and the hydrophobic solvent supply unit flows a hydrophobic solvent into the flow channel by an externally-applied force.
    Type: Grant
    Filed: August 29, 2013
    Date of Patent: October 24, 2017
    Assignee: The University of Tokyo
    Inventors: Soo Hyeon Kim, Hiroyuki Noji, Ryota Iino, Jun Ohta, Takashi Tokuda, Kiyotaka Sasagawa, Toshihiko Noda
  • Publication number: 20170227538
    Abstract: Provided is a method to detect a target molecule, including a complex formation step of reacting a target molecule, a carrier modified with a first antibody which specifically binds to the target molecule, and two or more second antibodies which specifically bind to the target molecule, and are labeled with enzymes having a substrate cleaving activity and mutually different substrate specificities, to form a complex consisting of the first antibody, the target molecule and the second antibodies, on the carrier; and a detection step of reacting two or more substrates having cleavage sites by the enzymes, a fluorescent substance bound to one terminal side of each of the cleavage sites and a quencher bound to another terminal side thereof, wherein the fluorescent substances are mutually different in fluorescence wavelength, and the complex, to detect fluorescence emitted from the fluorescent substances.
    Type: Application
    Filed: September 9, 2015
    Publication date: August 10, 2017
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventor: Hiroyuki NOJI
  • Publication number: 20170176430
    Abstract: As a technique for efficiently sealing many substances, such as beads, nucleic acid, protein, virus, cells, and lipid membrane complex, into an array, the present invention provides a method for sealing a substance, including: (i) a step of introducing a first solvent containing a substance on a substrate on which a plurality of receptacles capable of storing the substance are formed separated from each other by a side wall; and (ii) a step of introducing a second solvent having a greater specific gravity than that of the first solvent onto the first solvent, the step (ii) being carried out after the step (i).
    Type: Application
    Filed: July 1, 2015
    Publication date: June 22, 2017
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki NOJI, Lisa YAMAUCHI
  • Publication number: 20170115284
    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
    Type: Application
    Filed: November 15, 2016
    Publication date: April 27, 2017
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki NOJI, Ryota IINO, Suguru ARAKI
  • Publication number: 20160296928
    Abstract: A material film is formed as a thin film having a thickness of 1 ?m on a surface of a glass substrate. A plurality of micro-chambers having a diameter of 5 ?m are formed in the material film to be arrayed at a high density. The respective chambers filled with an aqueous test solution have openings that are liquid-sealed by a lipid bilayer membrane to provide a high-density micro-chamber array. Significant downsizing of the micro-chambers enhances a change in concentration by a reaction of one biomolecule in the chamber and thereby increases the detection sensitivity. In the configuration that a large number of micro-chambers are formed at a high density, even in the case of an extremely slow reaction of the biomolecule, the reaction proceeds in any of the chambers. This configuration accordingly enables the reaction of the biomolecule to be detected with high sensitivity.
    Type: Application
    Filed: August 18, 2014
    Publication date: October 13, 2016
    Applicant: THE UNIVERSITY OF TOKYO
    Inventors: Hiroyuki NOJI, Rikiya WATANABE, Hiroaki SUGA, Daishi FUJITA