Patents by Inventor Horst Wolff

Horst Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230196533
    Abstract: In a method for reducing an image vignetting, a microscope image is captured by means of a microscope, wherein the microscope image exhibits a vignetting. At least the microscope image is input into an image processing model which has been trained, by means of training data comprising input images with vignetting and associated target images without vignetting, to calculate a vignetting-free microscope image from at least the input microscope image. The method further comprises the outputting of a calculated vignetting-free microscope image by the image processing model.
    Type: Application
    Filed: December 16, 2022
    Publication date: June 22, 2023
    Inventors: Horst Wolff, Rebecca Elsaesser
  • Patent number: 9690087
    Abstract: A method for defining a z-range in a sample in which a z-stack of the sample is to be recorded by means of a microscope is provided, wherein the z-range is defined automatically on the basis of a z-value situated in the sample and taking at least one predetermined parameter into account.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: June 27, 2017
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Horst Wolff, Daniel Svejdar, Markus Eichinger
  • Publication number: 20140232844
    Abstract: A method for defining a z-range in a sample in which a z-stack of the sample is to be recorded by means of a microscope is provided, wherein the z-range is defined automatically on the basis of a z-value situated in the sample and taking at least one predetermined parameter into account.
    Type: Application
    Filed: April 4, 2012
    Publication date: August 21, 2014
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Horst Wolff, Daniel Svejdar, Markus Eichinger
  • Publication number: 20140118528
    Abstract: A setting unit for sequencing automatic recording of images with a recording device. The setting unit provides a first input interface via which a recording program can be set, provides a second input interface via which at least one parameter, which is to be monitored during the execution of the sequence, is proposed and can be selected in dependence on the recording program, provides a third input interface via which at least one condition, and at least one action upon fulfilment of the condition, can be set for the parameter, and generates control data for the control module.
    Type: Application
    Filed: October 28, 2013
    Publication date: May 1, 2014
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Horst Wolff, Daniel Svejdar, Markus Eichinger, Sebastian Albrecht, Moritz Ringler, Helmut Zöphel
  • Publication number: 20120249770
    Abstract: A method for automatic focusing of a microscope on a predetermined object in a specimen to be examined may include a) producing a set of criteria to be satisfied for the predetermined object using at least one training image of the object, b) producing a first image of the specimen to be examined which contains the predetermined object with the microscope in a first focal position, c) ascertaining the section or sections of the first image, which in each case satisfies or satisfy the set of criteria according to step a), and defining each ascertained section as object area of the first image, d) producing further images of the specimen with the microscope in different focal positions, e) determining the optimum focal position(s) using the further images, wherein, for this purpose, in all images only the partial region or partial regions which correspond to the object area or object areas is/are evaluated, f) focusing the microscope on at least one of the optimum focal position(s) determined in step e).
    Type: Application
    Filed: August 20, 2010
    Publication date: October 4, 2012
    Applicant: CARL ZEISS MICROIMAGING GMBH
    Inventors: Peter Hamm, Karl-Hans Englmeier, Thomas Waschulzik, Horst Wolff
  • Publication number: 20080161199
    Abstract: The present invention refers to inventive fusion proteins, to a method for detecting protein-protein-interactions in living cells and cell lysates using these inventive fusion proteins. The present invention also refers to a screening method for identifying compounds suitable to modify, i.e. inhibit or enhance, protein-protein-interaction using these fusion proteins. Additionally a method for detecting cells is disclosed comprising an unknown protein that interacts with a known protein. Along with the inventive fusion proteins used for these methods encoding nucleic acids, corresponding vectors and host cells transfected accordingly are disclosed herewith.
    Type: Application
    Filed: December 22, 2005
    Publication date: July 3, 2008
    Applicant: GSF-Forschungszentrum fuer Umwelt und Gesundheit GmbH
    Inventors: Horst Wolff, Andrea Brebeck, Manja Ziegler, Ruth Brack-Werner
  • Patent number: 7183047
    Abstract: The present invention relates to a reporter gene construct for the detection of the HIV Rev and HIV Tat proteins. Furthermore, the invention relates to a functionality test method for Rev and Rev fusion proteins prepared in a recombinant manner, a method of screening for sequences of different origin for their activity as an instability element, a method of screening for sequences which cause the transport out of the nucleus into the cytoplasm by binding to cellular or other viral shuttle proteins, as well as to a method for the detection of HIV-infected cells. The reporter gene construct according to the present invention, after it has been introduced into cells, in the presence of HIV Rev and HIV Tat proteins results in the formation of reporter proteins which may be used for quantitative/qualitative detection of the HIV Rev and HIV Tat proteins.
    Type: Grant
    Filed: June 20, 2002
    Date of Patent: February 27, 2007
    Assignee: GSF-Forschungszentrum für Umwelt und Gesundheit, GmbH
    Inventors: Ruth Brack-Werner, Markus Neumann, Horst Wolff, Volker Erfle
  • Publication number: 20040137642
    Abstract: The invention relates to MHC tetramer fusion proteins, MHC monomer fusion proteins, DNA encoding a MHC fusion protein as well as RNA yielding after transcription a MHC monomer fusion protein. Furthermore, the invention discloses the use of a DsRed protein as an agent for the tetramerization of MHC molecules, methods for the preparation of fusion proteins containing MHC and DsRed, and methods for the preparation of MHC tetramers. Moreover, the invention describes methods for the examination of an antigen-specific cellular immune response, particularly for the detection of T lymphocytes carrying specific T cell receptors on their surfaces, the uses of the MHC monomers and tetramers prepared according to the invention as well as test systems containing the MHC monomers or MHC tetramers according to the invention.
    Type: Application
    Filed: October 9, 2003
    Publication date: July 15, 2004
    Inventors: Volker Erfle, Horst Wolff, Dirk Busch, Markus Neumann
  • Publication number: 20030092032
    Abstract: The present invention relates to a reporter gene construct for the detection of the HIV Rev and HIV Tat proteins. Furthermore, the invention relates to a functionality test method for Rev and Rev fusion proteins prepared in a recombinant manner, a method of screening for sequences of different origin for their activity as an instability element, a method of screening for sequences which cause the transport out of the nucleus into the cytoplasm by binding to cellular or other viral shuttle proteins, as well as to a method for the detection of HIV-infected cells. The reporter gene construct according to the present invention, after it has been introduced into cells, in the presence of HIV Rev and HIV Tat proteins results in the formation of reporter proteins which may be used for quantitative/qualitative detection of the HIV Rev and HIV Tat proteins.
    Type: Application
    Filed: June 20, 2002
    Publication date: May 15, 2003
    Inventors: Ruth Brack-Werner, Markus Neumann, Horst Wolff, Volker Erfle
  • Patent number: 4583921
    Abstract: A pump which operates with several plungers is described with the plunger preferably being actuated via an eccentric drive unit and, having regard to the particular volume of flow per unit time, using several small plungers rather than a lesser number of comparatively large plungers, and accordingly operating with a large number of small volume displacements at a comparatively high speed of rotation. In this arrangement the suction chamber is centrally arranged in the pump body and the high pressure outlets of the pump chambers, which open into a common pressure chamber of ring-like construction, extend at least substantially in a radial direction.
    Type: Grant
    Filed: December 28, 1984
    Date of Patent: April 22, 1986
    Assignee: Speck-Kolbenpumpen-Fabrik Otto Speck KG
    Inventors: Horst Wolff, Franz Hani
  • Patent number: 4561828
    Abstract: A pump for forwarding liquids is described which has at least one pump chamber with inlet and outlet valves and also a plunger which periodically varies the volume of the pump chamber. To avoid the problems of dry running with very rapidly running pumps, and in order to overcome the resulting danger of destruction, the pump is arranged so that the closing force of at least one inlet valve of each pump chamber is controlled in dependence on the movement of the displacement member.
    Type: Grant
    Filed: May 18, 1983
    Date of Patent: December 31, 1985
    Assignee: Speck-Kolbenpumpen-Fabrik Otto Speck KG
    Inventors: Horst Wolff, Franz Hani
  • Patent number: 4456439
    Abstract: A high pressure plunger pump, in particular for forwarding water and water with additives, comprises a plurality of plungers 20 which are driven by a crankshaft journalled in a drive housing 10. The free ends of the plungers 20 are each guided in respective pump chambers 22 of an induction-discharge housing 12 which is connected to the drive housing 10. The induction-discharge housing 12 is a one piece housing of generally L-shaped cross-section. The pump chambers 22 are arranged in one limb of the L-shaped housing and communicate with an induction chamber 27 via spring biased inlet valves 27 provided in axial extensions of the pump chambers. A discharge chamber 31 extends through the other limb of the L-shaped housing and is fed from the pump chambers via outlet valves 29. Both the inlet and the outlet valves 25, 29 are secured in position by respective threaded plugs. The use of a one piece L-shaped housing brings a number of substantial benefits.
    Type: Grant
    Filed: November 4, 1981
    Date of Patent: June 26, 1984
    Assignee: Speck-Kolbenpumpen-Fabrik
    Inventor: Horst Wolff
  • Patent number: 4301275
    Abstract: In aqueous dispersions on the basis of polyvinyl chloride the content of residual monomers is reduced by the countercurrent method by contacting the rapidly moved surface of the dispersion with secluded intensely mixed gas atmospheres in several successive chambers. To carry out the process a device is used which permits to obtain under mild conditions residual monomer contents of 10 ppm and therebelow.
    Type: Grant
    Filed: May 19, 1980
    Date of Patent: November 17, 1981
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Christoph Heinze, Franz Botsch, Horst Wolff
  • Patent number: 4246924
    Abstract: A valve arrangement is disclosed for controlling the supply of an operating fluid, having a casing with an inlet port, a main flow path provided with a non-return valve extending from the inlet port to an operating outlet port and a secondary flow path extending from the inlet port to a by-pass outlet port, the secondary flow path containing a ball applied to a valve seat under spring prestressing to close said secondary flow path, there being provided a piston including a tappet operable to displace the ball from the seat, a spring for biasing the piston away from the ball, a flow path including a restrictor communicating pressure from the main flow path to said piston to displace the piston against the spring following an excessive rise in pressure in the main flow path, the structure permitting a predetermined stroke of the piston against the force of the spring prior to displacing the ball from its seat via the tappet.
    Type: Grant
    Filed: February 26, 1979
    Date of Patent: January 27, 1981
    Assignee: Speck Kolbenpumpenfabrik Otto Speck KG
    Inventors: Lutz Droitsch, Horst Wolff
  • Patent number: 4158092
    Abstract: Polymer dispersions are subject to a spray treatment to reduce the monomer content thereof. The dispersions are sprayed against the wall of the vessel by means of at least one spray nozzle.The residual monomer content of polymer dispersions is further reduced by spraying them in at least two series connected vessels, conducting the escaping gaseous constituents in one direction and the dispersion in opposite direction from one vessel to the next. The process is little liable to troubles and ensures a very effective monomer removal without thermal damage of the polymer.
    Type: Grant
    Filed: October 26, 1977
    Date of Patent: June 12, 1979
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Franz Botsch, Christoph Heinze, Horst Wolff
  • Patent number: 4090014
    Abstract: .alpha.-Ethylenically unsaturated monomers are polymerized in aqueous media in a vessel with electrically conductive inner surface, in which partial areas of the inner surface covered by liquid are electrically insulated from the remaining surface areas and conductively connected therewith by an external source of current. In this manner the formation of deposits on the wall of vessel is hindered or suppressed.
    Type: Grant
    Filed: August 20, 1976
    Date of Patent: May 16, 1978
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Hermann Scheibler, Kasimir Ruchlak, Christoph Heinze, Horst Wolff
  • Patent number: 4009536
    Abstract: A trigger mechanism for firing a firearm comprises an electromagnet including a coil and a movable armature actuated by the coil. The armature is connected to the firing pin so as to move the firing pin when the coil is energized by actuating switch means which connects the coil to a source of electrical energy. The switch means is actuated by a trigger lever or button which closes a normally open switch.
    Type: Grant
    Filed: January 29, 1975
    Date of Patent: March 1, 1977
    Assignee: Carl Walther Sportwaffenfabrik
    Inventor: Horst Wolff