Patents by Inventor Horst Wolff
Horst Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230196533Abstract: In a method for reducing an image vignetting, a microscope image is captured by means of a microscope, wherein the microscope image exhibits a vignetting. At least the microscope image is input into an image processing model which has been trained, by means of training data comprising input images with vignetting and associated target images without vignetting, to calculate a vignetting-free microscope image from at least the input microscope image. The method further comprises the outputting of a calculated vignetting-free microscope image by the image processing model.Type: ApplicationFiled: December 16, 2022Publication date: June 22, 2023Inventors: Horst Wolff, Rebecca Elsaesser
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Patent number: 9690087Abstract: A method for defining a z-range in a sample in which a z-stack of the sample is to be recorded by means of a microscope is provided, wherein the z-range is defined automatically on the basis of a z-value situated in the sample and taking at least one predetermined parameter into account.Type: GrantFiled: April 4, 2012Date of Patent: June 27, 2017Assignee: Carl Zeiss Microscopy GmbHInventors: Horst Wolff, Daniel Svejdar, Markus Eichinger
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Publication number: 20140232844Abstract: A method for defining a z-range in a sample in which a z-stack of the sample is to be recorded by means of a microscope is provided, wherein the z-range is defined automatically on the basis of a z-value situated in the sample and taking at least one predetermined parameter into account.Type: ApplicationFiled: April 4, 2012Publication date: August 21, 2014Applicant: Carl Zeiss Microscopy GmbHInventors: Horst Wolff, Daniel Svejdar, Markus Eichinger
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Publication number: 20140118528Abstract: A setting unit for sequencing automatic recording of images with a recording device. The setting unit provides a first input interface via which a recording program can be set, provides a second input interface via which at least one parameter, which is to be monitored during the execution of the sequence, is proposed and can be selected in dependence on the recording program, provides a third input interface via which at least one condition, and at least one action upon fulfilment of the condition, can be set for the parameter, and generates control data for the control module.Type: ApplicationFiled: October 28, 2013Publication date: May 1, 2014Applicant: Carl Zeiss Microscopy GmbHInventors: Horst Wolff, Daniel Svejdar, Markus Eichinger, Sebastian Albrecht, Moritz Ringler, Helmut Zöphel
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Publication number: 20120249770Abstract: A method for automatic focusing of a microscope on a predetermined object in a specimen to be examined may include a) producing a set of criteria to be satisfied for the predetermined object using at least one training image of the object, b) producing a first image of the specimen to be examined which contains the predetermined object with the microscope in a first focal position, c) ascertaining the section or sections of the first image, which in each case satisfies or satisfy the set of criteria according to step a), and defining each ascertained section as object area of the first image, d) producing further images of the specimen with the microscope in different focal positions, e) determining the optimum focal position(s) using the further images, wherein, for this purpose, in all images only the partial region or partial regions which correspond to the object area or object areas is/are evaluated, f) focusing the microscope on at least one of the optimum focal position(s) determined in step e).Type: ApplicationFiled: August 20, 2010Publication date: October 4, 2012Applicant: CARL ZEISS MICROIMAGING GMBHInventors: Peter Hamm, Karl-Hans Englmeier, Thomas Waschulzik, Horst Wolff
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Publication number: 20080161199Abstract: The present invention refers to inventive fusion proteins, to a method for detecting protein-protein-interactions in living cells and cell lysates using these inventive fusion proteins. The present invention also refers to a screening method for identifying compounds suitable to modify, i.e. inhibit or enhance, protein-protein-interaction using these fusion proteins. Additionally a method for detecting cells is disclosed comprising an unknown protein that interacts with a known protein. Along with the inventive fusion proteins used for these methods encoding nucleic acids, corresponding vectors and host cells transfected accordingly are disclosed herewith.Type: ApplicationFiled: December 22, 2005Publication date: July 3, 2008Applicant: GSF-Forschungszentrum fuer Umwelt und Gesundheit GmbHInventors: Horst Wolff, Andrea Brebeck, Manja Ziegler, Ruth Brack-Werner
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Patent number: 7183047Abstract: The present invention relates to a reporter gene construct for the detection of the HIV Rev and HIV Tat proteins. Furthermore, the invention relates to a functionality test method for Rev and Rev fusion proteins prepared in a recombinant manner, a method of screening for sequences of different origin for their activity as an instability element, a method of screening for sequences which cause the transport out of the nucleus into the cytoplasm by binding to cellular or other viral shuttle proteins, as well as to a method for the detection of HIV-infected cells. The reporter gene construct according to the present invention, after it has been introduced into cells, in the presence of HIV Rev and HIV Tat proteins results in the formation of reporter proteins which may be used for quantitative/qualitative detection of the HIV Rev and HIV Tat proteins.Type: GrantFiled: June 20, 2002Date of Patent: February 27, 2007Assignee: GSF-Forschungszentrum für Umwelt und Gesundheit, GmbHInventors: Ruth Brack-Werner, Markus Neumann, Horst Wolff, Volker Erfle
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Publication number: 20040137642Abstract: The invention relates to MHC tetramer fusion proteins, MHC monomer fusion proteins, DNA encoding a MHC fusion protein as well as RNA yielding after transcription a MHC monomer fusion protein. Furthermore, the invention discloses the use of a DsRed protein as an agent for the tetramerization of MHC molecules, methods for the preparation of fusion proteins containing MHC and DsRed, and methods for the preparation of MHC tetramers. Moreover, the invention describes methods for the examination of an antigen-specific cellular immune response, particularly for the detection of T lymphocytes carrying specific T cell receptors on their surfaces, the uses of the MHC monomers and tetramers prepared according to the invention as well as test systems containing the MHC monomers or MHC tetramers according to the invention.Type: ApplicationFiled: October 9, 2003Publication date: July 15, 2004Inventors: Volker Erfle, Horst Wolff, Dirk Busch, Markus Neumann
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Publication number: 20030092032Abstract: The present invention relates to a reporter gene construct for the detection of the HIV Rev and HIV Tat proteins. Furthermore, the invention relates to a functionality test method for Rev and Rev fusion proteins prepared in a recombinant manner, a method of screening for sequences of different origin for their activity as an instability element, a method of screening for sequences which cause the transport out of the nucleus into the cytoplasm by binding to cellular or other viral shuttle proteins, as well as to a method for the detection of HIV-infected cells. The reporter gene construct according to the present invention, after it has been introduced into cells, in the presence of HIV Rev and HIV Tat proteins results in the formation of reporter proteins which may be used for quantitative/qualitative detection of the HIV Rev and HIV Tat proteins.Type: ApplicationFiled: June 20, 2002Publication date: May 15, 2003Inventors: Ruth Brack-Werner, Markus Neumann, Horst Wolff, Volker Erfle
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Patent number: 4583921Abstract: A pump which operates with several plungers is described with the plunger preferably being actuated via an eccentric drive unit and, having regard to the particular volume of flow per unit time, using several small plungers rather than a lesser number of comparatively large plungers, and accordingly operating with a large number of small volume displacements at a comparatively high speed of rotation. In this arrangement the suction chamber is centrally arranged in the pump body and the high pressure outlets of the pump chambers, which open into a common pressure chamber of ring-like construction, extend at least substantially in a radial direction.Type: GrantFiled: December 28, 1984Date of Patent: April 22, 1986Assignee: Speck-Kolbenpumpen-Fabrik Otto Speck KGInventors: Horst Wolff, Franz Hani
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Patent number: 4561828Abstract: A pump for forwarding liquids is described which has at least one pump chamber with inlet and outlet valves and also a plunger which periodically varies the volume of the pump chamber. To avoid the problems of dry running with very rapidly running pumps, and in order to overcome the resulting danger of destruction, the pump is arranged so that the closing force of at least one inlet valve of each pump chamber is controlled in dependence on the movement of the displacement member.Type: GrantFiled: May 18, 1983Date of Patent: December 31, 1985Assignee: Speck-Kolbenpumpen-Fabrik Otto Speck KGInventors: Horst Wolff, Franz Hani
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Patent number: 4456439Abstract: A high pressure plunger pump, in particular for forwarding water and water with additives, comprises a plurality of plungers 20 which are driven by a crankshaft journalled in a drive housing 10. The free ends of the plungers 20 are each guided in respective pump chambers 22 of an induction-discharge housing 12 which is connected to the drive housing 10. The induction-discharge housing 12 is a one piece housing of generally L-shaped cross-section. The pump chambers 22 are arranged in one limb of the L-shaped housing and communicate with an induction chamber 27 via spring biased inlet valves 27 provided in axial extensions of the pump chambers. A discharge chamber 31 extends through the other limb of the L-shaped housing and is fed from the pump chambers via outlet valves 29. Both the inlet and the outlet valves 25, 29 are secured in position by respective threaded plugs. The use of a one piece L-shaped housing brings a number of substantial benefits.Type: GrantFiled: November 4, 1981Date of Patent: June 26, 1984Assignee: Speck-Kolbenpumpen-FabrikInventor: Horst Wolff
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Patent number: 4301275Abstract: In aqueous dispersions on the basis of polyvinyl chloride the content of residual monomers is reduced by the countercurrent method by contacting the rapidly moved surface of the dispersion with secluded intensely mixed gas atmospheres in several successive chambers. To carry out the process a device is used which permits to obtain under mild conditions residual monomer contents of 10 ppm and therebelow.Type: GrantFiled: May 19, 1980Date of Patent: November 17, 1981Assignee: Hoechst AktiengesellschaftInventors: Christoph Heinze, Franz Botsch, Horst Wolff
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Patent number: 4246924Abstract: A valve arrangement is disclosed for controlling the supply of an operating fluid, having a casing with an inlet port, a main flow path provided with a non-return valve extending from the inlet port to an operating outlet port and a secondary flow path extending from the inlet port to a by-pass outlet port, the secondary flow path containing a ball applied to a valve seat under spring prestressing to close said secondary flow path, there being provided a piston including a tappet operable to displace the ball from the seat, a spring for biasing the piston away from the ball, a flow path including a restrictor communicating pressure from the main flow path to said piston to displace the piston against the spring following an excessive rise in pressure in the main flow path, the structure permitting a predetermined stroke of the piston against the force of the spring prior to displacing the ball from its seat via the tappet.Type: GrantFiled: February 26, 1979Date of Patent: January 27, 1981Assignee: Speck Kolbenpumpenfabrik Otto Speck KGInventors: Lutz Droitsch, Horst Wolff
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Patent number: 4158092Abstract: Polymer dispersions are subject to a spray treatment to reduce the monomer content thereof. The dispersions are sprayed against the wall of the vessel by means of at least one spray nozzle.The residual monomer content of polymer dispersions is further reduced by spraying them in at least two series connected vessels, conducting the escaping gaseous constituents in one direction and the dispersion in opposite direction from one vessel to the next. The process is little liable to troubles and ensures a very effective monomer removal without thermal damage of the polymer.Type: GrantFiled: October 26, 1977Date of Patent: June 12, 1979Assignee: Hoechst AktiengesellschaftInventors: Franz Botsch, Christoph Heinze, Horst Wolff
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Patent number: 4090014Abstract: .alpha.-Ethylenically unsaturated monomers are polymerized in aqueous media in a vessel with electrically conductive inner surface, in which partial areas of the inner surface covered by liquid are electrically insulated from the remaining surface areas and conductively connected therewith by an external source of current. In this manner the formation of deposits on the wall of vessel is hindered or suppressed.Type: GrantFiled: August 20, 1976Date of Patent: May 16, 1978Assignee: Hoechst AktiengesellschaftInventors: Hermann Scheibler, Kasimir Ruchlak, Christoph Heinze, Horst Wolff
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Patent number: 4009536Abstract: A trigger mechanism for firing a firearm comprises an electromagnet including a coil and a movable armature actuated by the coil. The armature is connected to the firing pin so as to move the firing pin when the coil is energized by actuating switch means which connects the coil to a source of electrical energy. The switch means is actuated by a trigger lever or button which closes a normally open switch.Type: GrantFiled: January 29, 1975Date of Patent: March 1, 1977Assignee: Carl Walther SportwaffenfabrikInventor: Horst Wolff