Patents by Inventor Hubert Koster

Hubert Koster has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7759065
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. In some embodiments, a process comprises: amplifying one or more target nucleic acid molecules from a biological sample; ionizing and volatilizing the amplified product; and analyzing a product by mass spectrometry to determine an observed molecular mass of the product.
    Type: Grant
    Filed: May 22, 2008
    Date of Patent: July 20, 2010
    Assignee: Sequenom, Inc.
    Inventor: Hubert Köster
  • Patent number: 7668658
    Abstract: Processes and methods for creating a database of genomic samples from healthy human donors, methods that use the database to identify and correlate polymorphic genetic markers and other markers with diseases and conditions are provided.
    Type: Grant
    Filed: October 15, 2002
    Date of Patent: February 23, 2010
    Assignee: Sequenom, Inc.
    Inventors: Hubert Köster, Andreas Braun, Dirk Van Den Boom, Yip Ping, Charlie Rodi, Liyan He, Norman Chiu, Christian Jurinke
  • Publication number: 20090092977
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided.
    Type: Application
    Filed: May 22, 2008
    Publication date: April 9, 2009
    Applicant: SEQUENOM, INC.
    Inventor: Hubert Koster
  • Patent number: 7501251
    Abstract: Provided are methods for detecting a target nucleic acid in a biological sample using RNA amplification. In an embodiment, provided is a method that comprises (a) amplifying a target nucleic acid, or portion thereof, using a primer comprising a sequence that is complementary to a polynucleotide sequence in the target nucleic acid, or a complement thereof, and a sequence that encodes an RNA polymerase promoter; (b) synthesizing RNA using an RNA polymerase that recognizes the promoter; and (c) detecting the presence or absence of the resulting RNA using mass spectrometry thereby detecting the presence or absence of the target nucleic acid in the biological sample.
    Type: Grant
    Filed: October 2, 2006
    Date of Patent: March 10, 2009
    Assignee: Sequenom, Inc.
    Inventors: Hubert Köster, Andreas Braun, Dirk van den Boom, Christian Jurinke, Andreas Ruppert, Franz Hillenkamp
  • Publication number: 20090042203
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided.
    Type: Application
    Filed: June 27, 2008
    Publication date: February 12, 2009
    Applicant: SEQUENOM, INC.
    Inventor: Hubert Koster
  • Publication number: 20090023150
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.
    Type: Application
    Filed: June 27, 2008
    Publication date: January 22, 2009
    Applicant: SEQUENOM, INC.
    Inventors: Hubert Koster, Daniel P. Little, Andreas Braun, David M. Lough, Guobing Xiang, Dirk van den Boom, Christian Jurinke, Andreas Ruppert
  • Publication number: 20080248968
    Abstract: Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.
    Type: Application
    Filed: May 19, 2008
    Publication date: October 9, 2008
    Applicant: Sequenom, Inc.
    Inventors: Daniel P. Little, Maryanne J. O'Donnell-Maloney, Charles R. Cantor, Hubert Koster
  • Patent number: 7419787
    Abstract: Provided herein in certain embodiments are processes that generally include the following steps: amplifying a target nucleic acid molecule; ionizing and volatilizing the amplification product and analyzing the ionized and volatilized product by mass spectrometry. In some embodiments the amplification product is a natural nucleic acid and detection of the target nucleotide sequence by mass spectrometry indicates the presence of the target nucleotide sequence in the biological sample.
    Type: Grant
    Filed: May 11, 2006
    Date of Patent: September 2, 2008
    Assignee: Sequenom, Inc.
    Inventor: Hubert Köster
  • Patent number: 7390672
    Abstract: Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.
    Type: Grant
    Filed: June 18, 2007
    Date of Patent: June 24, 2008
    Assignee: Sequenom, Inc.
    Inventors: Daniel P. Little, Maryanne J. O'Donnell-Maloney, Charles R. Cantor, Hubert Köster
  • Publication number: 20080008874
    Abstract: Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.
    Type: Application
    Filed: June 18, 2007
    Publication date: January 10, 2008
    Applicant: Sequenom, Inc.
    Inventors: Daniel Little, Maryanne O'Donnell-Maloney, Charles Cantor, Hubert Koster
  • Patent number: 7285422
    Abstract: The invention provides methods for dispensing tools that can be employed to generate multi-element arrays of sample material on a substrate surface. The substrates surfaces can be flat or geometrically altered to include wells of receiving material. The tool can dispense a spot of fluid to a substrate surface by spraying the fluid from the pin, contacting the substrate surface or forming a drop that touches against the substrate surface. The tool can form an array of sample material by dispensing sample material in a series of steps, while moving the pin to different locations above the substrate surface to form the sample array. The invention then passes the prepared sample arrays to a plate assembly that disposes the sample arrays for analysis by mass spectrometry. To this end, a mass spectrometer is provided that generates a set of spectra signal which can be understood as indicative of the composition of the sample material under analysis.
    Type: Grant
    Filed: January 23, 1997
    Date of Patent: October 23, 2007
    Assignee: Sequenom, Inc.
    Inventors: Daniel P. Little, Maryanne J. O'Donnell-Maloney, Charles R. Cantor, Hubert Köster
  • Publication number: 20070202514
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.
    Type: Application
    Filed: October 2, 2006
    Publication date: August 30, 2007
    Applicant: Sequenom, Inc.
    Inventors: Hubert Koster, Andreas Braun, Dirk Van Den Boom, Christian Jurinke, Andreas Ruppert, Franz Hillenkamp
  • Patent number: 7232688
    Abstract: Methods for dispensing tools that can be employed to generate multi-element arrays of sample material on a substrate surface. The resulting substrates are also provided. The substrates surfaces can be flat or geometrically altered to include wells of receiving material. The tool can dispense a spot of fluid to a substrate surface by spraying the fluid from the pin, contacting the substrate surface or forming a drop that touches against the substrate surface. The tool can form an array of sample material by dispensing sample material in a series of steps, while moving the pin to different locations above the substrate surface to form the sample array. The prepared sample arrays are passed to a plate assembly that disposes the sample arrays for analysis by mass spectrometry. To this end, a mass spectrometer is provided that generates a set of spectra signals that are indicative of the composition of the sample material under analysis.
    Type: Grant
    Filed: July 30, 1999
    Date of Patent: June 19, 2007
    Assignee: Sequenom, Inc.
    Inventors: Daniel P. Little, Maryanne J. O'Donnell-Maloney, Charles R. Cantor, Hubert Köster
  • Patent number: 7198893
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.
    Type: Grant
    Filed: October 10, 2000
    Date of Patent: April 3, 2007
    Assignee: Sequenom, Inc.
    Inventors: Hubert Köster, Andreas Braun, Dirk van den Boom, Christian Jurinke, Andreas Ruppert, Franz Hillenkamp, Daniel P. Little
  • Publication number: 20060223105
    Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided.
    Type: Application
    Filed: May 11, 2006
    Publication date: October 5, 2006
    Inventor: Hubert Koster
  • Patent number: 7094943
    Abstract: Multifunctional liquid phase carriers (LPCs) and methods of using LPCs for the preparation of biopolymers are provided. The LPCs are highly symmetrical compounds that possess more than two points of attachment for biopolymer synthesis. The LPCs have the formula Sp(X1)n, where Sp is a highly symmetrical moiety such that all X1 groups are equivalent. X1 is a functional group that is suitable for biopolymer synthesis, including OH, SH, NH2, COOH and the like. Biopolymers that may be produced using the methods provided include oligonucleotides, peptides, protein nucleic acids (PNAs) and oligosaccharides. Analogs of the biopolymers may also be prepared using the methods.
    Type: Grant
    Filed: April 27, 1998
    Date of Patent: August 22, 2006
    Inventors: Hubert Köster, Ralf Wörl
  • Patent number: 7074563
    Abstract: Provided herein are mass spectrometric methods for detecting a mutation in a target nucleic acid. In an embodiment, provided is a method which comprises digesting a target nucleic acid molecule; capturing digested fragments on a solid support that comprises oligonucleotides complementary thereto; and detecting hybrids and the molecular weights of captured fragments by mass spectrometry, thereby identifying mutations in the target nucleic acid molecule.
    Type: Grant
    Filed: February 24, 2003
    Date of Patent: July 11, 2006
    Assignee: Sequenom, Inc.
    Inventor: Hubert Köster
  • Patent number: 7038103
    Abstract: Multifunctional liquid phase carriers (LPCs) and methods of using LPCs for the preparation of biopolymers are provided. The LPCs are highly symmetrical compounds that possess more than two points of attachment for biopolymer synthesis. The LPCs have the formula Sp(X1)n, where Sp is a highly symmetrical moiety such that all X1 groups are equivalent. X1 is a functional group that is suitable for biopolymer synthesis, including OH, SH, NH2, COOH and the like. Biopolymers that may be produced using the methods provided include oligonucleotides, peptides, protein nucleic acids (PNAs) and oligosaccharides. Analogs of the biopolymers may also be prepared using the methods.
    Type: Grant
    Filed: January 18, 2000
    Date of Patent: May 2, 2006
    Assignee: Hubert Koster
    Inventors: Hubert Köster, Ralf Wörl
  • Publication number: 20060063193
    Abstract: Methods for detecting target nucleic acid molecules in a sample are provided. The methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes include a double-stranded portion, a single-stranded portion and a variable sequence within the single-stranded portion, where the single-stranded region of the probes includes a sequence complementary or homologous to a sequence of the target nucleic acid to be detected. The molecular weights of the hybridized nucleic acids of the set are determined by mass spectroscopy, and from the molecular weights of the hybridized probes, the presence of the target nucleic acid is detected by the presence of its sequence in the sample.
    Type: Application
    Filed: October 25, 2005
    Publication date: March 23, 2006
    Inventors: Dong-Jing Fu, Charles Canton, Hubert Koster, Cassandra Smith
  • Patent number: RE41005
    Abstract: Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to “flat” surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.
    Type: Grant
    Filed: October 17, 2002
    Date of Patent: November 24, 2009
    Assignee: Sequenom, Inc.
    Inventors: Hubert Koster, David M. Lough