Abstract: An aperture device for a flow cytometer is described. The aperture device includes a first aperture, and a second aperture spaced apart from the first aperture by a distance. The distance between the first and second apertures and a size of the first and second apertures are adjustable to create an optimal light intensity fringe pattern for analyzing a particle having a preselected type or characteristic at an interrogation zone of the flow cytometer.

Abstract: Aspects of the present disclosure include flow cytometers configured to compensate for optical noise caused by operational change of a laser. Flow cytometers according to certain embodiments include a laser assessor configured to assess operational change of a laser. In embodiments, the laser assessor includes a reference detector and a mirror positioned between the reference detector and the flow cell that is configured to reflect forward scattered light to a forward scatter detector and allow non-scattered laser light to pass through to the reference detector. Methods for assessing laser functionality and, where desired, dynamically adjusting flow cytometer data, based on laser reference data from the laser assessor are also provided.

Abstract: A portable weather station, including an lower body portion; an upper body portion disposed on the lower body portion in a spaced apart relationship thereby forming an open channel between the upper body portion and the lower body portion; and a plurality of weather condition sensors wherein a first set of one or more of the plurality of weather condition sensors is mounted on the upper body portion of the portable weather station and a second set of one or more of the plurality of weather condition sensors is mounted on the lower body portion of the portable weather station.

Abstract: A portable weather station, including an lower body portion; an upper body portion disposed on the lower body portion in a spaced apart relationship thereby forming an open channel between the upper body portion and the lower body portion; and a plurality of weather condition sensors wherein a first set of one or more of the plurality of weather condition sensors is mounted on the upper body portion of the portable weather station and a second set of one or more of the plurality of weather condition sensors is mounted on the lower body portion of the portable weather station.

Abstract: Systems are provided for delivering a calibrated ultraviolet radiation pulse at a treatment plane during a laser-ablation treatment of a patient's eye. Exemplary systems include an ultraviolet radiation source, and a fluorescent plate positioned to receive an initial ultraviolet radiation pulse produced by the ultraviolet radiation source. The fluorescent plate generates a visible light pulse in response to the initial ultraviolet radiation pulse.

Abstract: Embodiments of the present invention generally describe systems, devices, and methods for directly measuring pulse profiles during pulse delivery. In some embodiment, the pulse profiles may be measured while the pulse is delivered to ablate a material. Embodiments, may calculate ablation spot parameters based on the pulse profiles and may refine one or more subsequent laser pulses based on deviations from the calculated ablation spot parameters from desired ablation spot parameters. In some embodiments, a fluence profiler is provided. The fluence profiler may measure a pulse profile of a laser pulse from a portion of the laser pulse. The fluence profiler may utilize a UV radiation energy sensor device and a camera-based imager. The measurements from the UV radiation energy sensor device and the camera-based imager may be combined and scaled to provide a measured pulse profile that corresponds to the delivered pulse.

Abstract: A portable weather station, including an lower body portion; an upper body portion disposed on the lower body portion in a spaced apart relationship thereby forming an open channel between the upper body portion and the lower body portion; and a plurality of weather condition sensors wherein a first set of one or more of the plurality of weather condition sensors is mounted on the upper body portion of the portable weather station and a second set of one or more of the plurality of weather condition sensors is mounted on the lower body portion of the portable weather station.

Abstract: A laser pulse energy control system which includes a laser source and a beam divider positioned to receive a calibration laser pulse produced by the laser source. The beam divider reflects a first portion of the calibration laser pulse along a first optical path toward a first plane and transmits a second portion of the calibration laser pulse along a second optical path toward a second plane. An energy meter determines an energy of the first portion of the calibration laser pulse at the first plane and a fluence profiler determines a fluence profile of the second portion of the calibration laser pulse at the second plane. The processor controls an energy of an ablation laser pulse produced by the laser source based on the fluence profile of the second portion of the calibration laser pulse and the energy of the first portion of the calibration laser pulse.

Abstract: Systems are provided for delivering a calibrated ultraviolet radiation pulse at a treatment plane during a laser-ablation treatment of a patient's eye. Exemplary systems include an ultraviolet radiation source, and a fluorescent plate positioned to receive an initial ultraviolet radiation pulse produced by the ultraviolet radiation source. The fluorescent plate generates a visible light pulse in response to the initial ultraviolet radiation pulse.

Abstract: A portable weather station, including an lower body portion; an upper body portion disposed on the lower body portion in a spaced apart relationship thereby forming an open channel between the upper body portion and the lower body portion; and a plurality of weather condition sensors wherein a first set of one or more of the plurality of weather condition sensors is mounted on the upper body portion of the portable weather station and a second set of one or more of the plurality of weather condition sensors is mounted on the lower body portion of the portable weather station.

Abstract: Embodiments generally describe systems, devices, and methods for focusing and calibrating beam profilers. A test object is provided that may include an internal housing rotatable within an external housing. The internal housing may house a light source, a collimator, a filter, and/or a diffuser. A plate may be mounted to the internal housing and may include a plurality of markings. In some embodiments, to focus a beam profiler, the test object may be positioned adjacent the converter plate of a beam profiler. Marker images may be captured and a focus quality may be assessed therefrom. A position of the converter, objective, and/or camera of the beam profiler may be adjusted based on the focus quality. To calibrate, images of the markings in several rotational positions may be captured and used for calibration. The markings may be rotated to several positions by rotating the internal housing relative to the external housing.

Abstract: Embodiments generally describe systems, devices, and methods for focusing and calibrating beam profilers. A test object is provided that may include an internal housing rotatable within an external housing. The internal housing may house a light source, a collimator, a filter, and/or a diffuser. A plate may be mounted to the internal housing and may include a plurality of markings. In some embodiments, to focus a beam profiler, the test object may be positioned adjacent the converter plate of a beam profiler. Marker images may be captured and a focus quality may be assessed therefrom. A position of the converter, objective, and/or camera of the beam profiler may be adjusted based on the focus quality. To calibrate, images of the markings in several rotational positions may be captured and used for calibration. The markings may be rotated to several positions by rotating the internal housing relative to the external housing.

Abstract: Embodiments generally describe systems, devices, and methods for focusing and calibrating beam profilers. A test object is provided that may include an internal housing rotatable within an external housing. The internal housing may house a light source, a collimator, a filter, and/or a diffuser. A plate may be mounted to the internal housing and may include a plurality of markings. In some embodiments, to focus a beam profiler, the test object may be positioned adjacent the converter plate of a beam profiler. Marker images may be captured and a focus quality may be assessed therefrom. A position of the converter, objective, and/or camera of the beam profiler may be adjusted based on the focus quality. To calibrate, images of the markings in several rotational positions may be captured and used for calibration. The markings may be rotated to several positions by rotating the internal housing relative to the external housing.

Abstract: Embodiments of the present invention generally describe systems, devices, and methods for directly measuring pulse profiles during pulse delivery. In some embodiment, the pulse profiles may be measured while the pulse is delivered to ablate a material. Embodiments, may calculate ablation spot parameters based on the pulse profiles and may refine one or more subsequent laser pulses based on deviations from the calculated ablation spot parameters from desired ablation spot parameters. In some embodiments, a fluence profiler is provided. The fluence profiler may measure a pulse profile of a laser pulse from a portion of the laser pulse. The fluence profiler may utilize a UV radiation energy sensor device and a camera-based imager. The measurements from the UV radiation energy sensor device and the camera-based imager may be combined and scaled to provide a measured pulse profile that corresponds to the delivered pulse.

Abstract: Embodiments generally describe systems, devices, and methods for focusing and calibrating beam profilers. A test object is provided that may include an internal housing rotatable within an external housing. The internal housing may house a light source, a collimator, a filter, and/or a diffuser. A plate may be mounted to the internal housing and may include a plurality of markings. In some embodiments, to focus a beam profiler, the test object may be positioned adjacent the converter plate of a beam profiler. Marker images may be captured and a focus quality may be assessed therefrom. A position of the converter, objective, and/or camera of the beam profiler may be adjusted based on the focus quality. To calibrate, images of the markings in several rotational positions may be captured and used for calibration. The markings may be rotated to several positions by rotating the internal housing relative to the external housing.