Abstract: It is an object of the present disclosure to provide a method for reducing the influence of baseline disturbances in a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR using a fluorescent dye, with a simple method. The present disclosure provides a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR, the method including: (1) amplifying the target nucleic acid in the dry blood filter paper by applying thermal cycles to a sample solution containing a dry blood filter paper punch piece and a PCR reagent, wherein the PCR reagent includes a fluorescently labeled probe; (2) optically detecting the fluorescence intensity of the sample solution for each of the thermal cycles; and (3) performing quantitative analysis of the target nucleic acid using data after a predetermined number of cycles of the optically detected data.

Abstract: A problem to be solved by the invention is to improve the sensitivity of measurement of an analyte in a sample by a sandwich immunoassay. The sensitivity can be improved significantly in a sandwich immunoassay using a first antibody and a second antibody when one or both of the first antibody and the second antibody are a mixture of a monoclonal antibody recognizing a linear epitope and a monoclonal antibody recognizing a conformational epitope.

Abstract: An object of the invention is to provide a primer for detecting and quantifying homozygous deletion of the SMN1 gene, the deletion of which causes SMA, using a dried blood spot in a filter paper and a method related to specific detection/quantification of the SMN1 gene using the primer.

Abstract: The present invention provides a cell culture vessel and a jig for fixing the cell culture vessel in position. More specifically, the cell culture vessel of the present invention is provided with a vessel portion having an annular sealed culture space when viewed from overhead and a port portion connecting the inner portion and outer portion of the culture space. The vessel portion is provided with a first vessel wall serving as the upper side during culturing and a second vessel wall serving as the lower side during culturing. Preferably at least the first vessel wall has flexibility.