Patents by Inventor Irina Morozova
Irina Morozova has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11674174Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: GrantFiled: January 6, 2020Date of Patent: June 13, 2023Assignees: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, ROCHE SEQUENCING SOLUTIONS, INC.Inventors: Sergey Kalachikov, Jingyue Ju, Irina Morozova, Michael Dorwart
-
Publication number: 20220372061Abstract: The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.Type: ApplicationFiled: July 9, 2020Publication date: November 24, 2022Applicant: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, Shiv Kumar, James Russo, Xiaoxu Li, Xin Chen, Minchen Chien, Steffen Jockusch, Xuanting Wang, Irina Morozova, Chuanjuan Tao, Sergey Kalachikov, Shundi Shi
-
Publication number: 20220364167Abstract: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-strandedType: ApplicationFiled: July 7, 2022Publication date: November 17, 2022Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Jingyue Ju, Shiv Kumar, James J. Russo, Steffen Jockusch, Zengmin Li, Xiaoxu Li, Sergey M. Kalachikov, Irina Morozova
-
Patent number: 11384391Abstract: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded.Type: GrantFiled: April 4, 2017Date of Patent: July 12, 2022Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Jingyue Ju, Shiv Kumar, James J. Russo, Steffen Jockusch, Zengmin Li, Xiaoxu Li, Sergey M. Kalachikov, Irina Morozova
-
Publication number: 20220177959Abstract: Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.Type: ApplicationFiled: March 5, 2020Publication date: June 9, 2022Applicant: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, Carl W. Fuller, Shiv Kumar, Sergey Kalachikov, Xiaoxu Li, Irina Morozova, James Russo, Steffen Jockusch
-
Patent number: 11225687Abstract: This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.Type: GrantFiled: April 4, 2017Date of Patent: January 18, 2022Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Jingyue Ju, Shiv Kumar, James J. Russo, Steffen Jockusch, Zengmin Li, Xiaoxu Li, Sergey Kalachikov, Irina Morozova
-
Publication number: 20210199641Abstract: The present application discloses novel and inventive methods, apparatuses, and the required molecules to detect compounds (analytes) utilizing antibodies or other protein-binding molecules and nanopore-detectable tags using specially designed nanopores, coupled with ionic current readout, to provide a single molecule electronic detection solution, offering unique opportunities for multiplexing and quantitation.Type: ApplicationFiled: January 22, 2021Publication date: July 1, 2021Applicant: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, James J. Russo, Sergey Kalachikov, Chuanjuan Tao, Shiv Kumar, Irina Morozova, Xiaoxu Li
-
Publication number: 20210139976Abstract: The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.Type: ApplicationFiled: March 14, 2019Publication date: May 13, 2021Applicant: The Trustees of Columbia University in the City of New YorkInventors: Jingyue Ju, Xiaoxu Li, Shiv Kumar, Xin Chen, James J. Russo, Minchen Chien, Steffen Jockusch, Chuanjuan Tao, Xuanting Wang, Sergey Kalachikov, Irina Morozova, Shundi Shi
-
Publication number: 20200248250Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides arc provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: January 6, 2020Publication date: August 6, 2020Applicants: The Trustees of Columbia University in the City of New York, Roche Sequencing Solutions, Inc.Inventors: Sergey Kalachikov, Jingyue Ju, Irina Morozova, Michael Dorwart
-
Patent number: 10526647Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: GrantFiled: March 3, 2017Date of Patent: January 7, 2020Assignees: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, ROCHE SEQUENCING SOLUTIONS, INC.Inventors: Sergey Kalachikov, Jingyue Ju, Irina Morozova, Michael Dorwart
-
Publication number: 20190153527Abstract: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-strandedType: ApplicationFiled: April 4, 2017Publication date: May 23, 2019Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Jingyue Ju, Shiv Kumar, James J. Russo, Steffen Jockusch, Zengmin Li, Xiaoxu Li, Sergey M. Kalachikov, Irina Morozova
-
Publication number: 20190112650Abstract: This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.Type: ApplicationFiled: April 4, 2017Publication date: April 18, 2019Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORKInventors: Jingyue Ju, Shiv Kumar, James J. Russo, Steffen Jockusch, Zengmin Li, Xiaoxu Li, Sergey Kalachikov, Irina Morozova
-
Publication number: 20170241948Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: March 3, 2017Publication date: August 24, 2017Applicants: The Trustees of Columbia University in the City of New York, Genia Technologies, Inc.Inventors: Sergey Kalachikov, Jingyue Ju, Irina Morozova, Michael Dorwart