Patents by Inventor IVANA BILUSIC-VILAGOS

IVANA BILUSIC-VILAGOS has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Conditional CRISPR sgRNA expression
    Patent number: 11884917
    Abstract: An expression cassette for conditional expression of a single guide RNA (sgRNA) of a CRISPR/Cas9 system, the cassette includes a promoter, an sgRNA sequence, and a sequence flanked by at least a pair of recombinase recognition sites, wherein recombinase activated re-combination at the pair of recombinase recognition sites is capable of excising said flanked sequence, whereby either i) at least one of said recombinase recognition sites is located within the sgRNA sequence and said flanked sequence contains an transcription disruption sequence or ii) said flanked sequence is at least a part of the promoter or of the sgRNA sequence; methods of conditional expression of sgRNA and a reaction product of the conditional expression, i.e. sgRNA with a recombination site remnant.
    Type: Grant
    Filed: March 17, 2017
    Date of Patent: January 30, 2024
    Assignees: IMBA—INSTITUT FÜR MOLEKULARE BIOTECHNOLOGIE GMBH, CAMPUS SCIENCE SUPPORT FACILITIES GMBH
    Inventors: Ulrich Elling, Krzysztof Chylinski, Maria Hubmann, Monika Borowska, Ivana Bilusic-Vilagos
  • CONDITIONAL CRISPR SGRNA EXPRESSION
    Publication number: 20190085325
    Abstract: An expression cassette for conditional expression of a single guide RNA (sgRNA) of a CRISPR/Cas9 system, the cassette includes a promoter, an sgRNA sequence, and a sequence flanked by at least a pair of recombinase recognition sites, wherein recombinase activated re-combination at the pair of recombinase recognition sites is capable of excising said flanked sequence, whereby either i) at least one of said recombinase recognition sites is located within the sgRNA sequence and said flanked sequence contains an transcription disruption sequence or ii) said flanked sequence is at least a part of the promoter or of the sgRNA sequence; methods of conditional expression of sgRNA and a reaction product of the conditional expression, i.e. sgRNA with a recombination site remnant.
    Type: Application
    Filed: March 17, 2017
    Publication date: March 21, 2019
    Inventors: Ulrich ELLING, Krzysztof CHYLINSKI, Maria HUBMANN, Monika BOROWSKA, IVANA BILUSIC-VILAGOS