Patents by Inventor J. Keith Joung

J. Keith Joung has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10415059
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: September 17, 2019
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Morgan Maeder, Yanfang Fu
  • Patent number: 10378027
    Abstract: Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 13, 2019
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Morgan Maeder
  • Publication number: 20190177374
    Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and
    Type: Application
    Filed: February 22, 2019
    Publication date: June 13, 2019
    Inventors: J. Keith Joung, Jeffry D. Sander
  • Patent number: 10273271
    Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and
    Type: Grant
    Filed: May 17, 2016
    Date of Patent: April 30, 2019
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander
  • Publication number: 20190106687
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Application
    Filed: August 22, 2018
    Publication date: April 11, 2019
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Publication number: 20190071657
    Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.
    Type: Application
    Filed: October 9, 2018
    Publication date: March 7, 2019
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Vikram Pattanayak
  • Patent number: 10202589
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Grant
    Filed: March 3, 2016
    Date of Patent: February 12, 2019
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Publication number: 20190010481
    Abstract: Engineered CRISPR from Prevotella and Francisella 1 (Cpf1) nucleases with improved targeting range and enhanced on-target activity, and their use in genomic engineering, epigenomic engineering, base editing, genome targeting, genome editing, and in vitro diagnostics.
    Type: Application
    Filed: April 23, 2018
    Publication date: January 10, 2019
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Alexander Sousa
  • Publication number: 20180340189
    Abstract: Many studies have shown that CRISPR-Cas nucleases can tolerate up to five mismatches and still cleave; it is hard to predict the effects of any given single or combination of mismatches on activity. Taken together, these nucleases can show significant off-target effects but it can be challenging to predict these sites. Described herein are methods for increasing the specificity of genome editing using the CRISPR/Cas system, e.g., using RNA-guided Foki Nucleases (RFNs), e.g., FokI-Cas9 or Foki-dCas9-based fusion proteins.
    Type: Application
    Filed: June 8, 2018
    Publication date: November 29, 2018
    Inventors: J. Keith Joung, Shengdar Tsai
  • Patent number: 10138476
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: November 27, 2018
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Shengdar Tsai
  • Patent number: 10138493
    Abstract: Embodiments disclosed herein provide artificial expression systems comprising the zinc-finger containing transcription factors and engineered promoters to modulate expression of genes of interest. Engineered zinc-finger transcription factors that interact with engineered promoters constitute synthetic and regulatable expression systems which facilitate the modulation of gene expression as desired.
    Type: Grant
    Filed: August 25, 2017
    Date of Patent: November 27, 2018
    Assignees: TRUSTEES OF BOSTON UNIVERSITY, THE GENERAL HOSPITAL CORPORATION
    Inventors: Ahmad S. Khalil, Divya Israni, Minhee Park, J. Keith Joung, Jeffry D. Sander
  • Patent number: 10119133
    Abstract: CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: November 6, 2018
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Jeffry D. Sander, Yan-fang Fu, Morgan Maeder
  • Patent number: 10093910
    Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.
    Type: Grant
    Filed: December 5, 2016
    Date of Patent: October 9, 2018
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Vikram Pattanayak
  • Publication number: 20180282714
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Application
    Filed: March 26, 2018
    Publication date: October 4, 2018
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Publication number: 20180265920
    Abstract: Sensitive, unbiased methods for genome-wide detection of potential off-target nuclease cleavage sites in DNA, e.g., in cell type-specific genomic DNA samples.
    Type: Application
    Filed: May 31, 2018
    Publication date: September 20, 2018
    Inventors: J. Keith Joung, Shengdar Tsai
  • Publication number: 20180245071
    Abstract: Sensitive, unbiased methods for genome-wide detection of potential CRISPR-Cas9 off-target cleavage sites from cell type-specific genomic DNA samples.
    Type: Application
    Filed: December 22, 2017
    Publication date: August 30, 2018
    Inventors: J. Keith Joung, Shengdar Tsai
  • Publication number: 20180230494
    Abstract: The present invention relates to methods to improve the absolute rate of homology-directed repair (HDR) and/or to improve the relative rate of HDR compared with non-homologous end joining (NHEJ).
    Type: Application
    Filed: October 1, 2015
    Publication date: August 16, 2018
    Inventors: J. Keith JOUNG, Benjamin KLEINSTIVER, Jason Michael GEHRKE, Shengdar TSAI, James ANGSTMAN, Rebecca Tayler COTTMAN
  • Publication number: 20180216088
    Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.
    Type: Application
    Filed: March 26, 2018
    Publication date: August 2, 2018
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Vikram Pattanayak
  • Publication number: 20180208921
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Application
    Filed: January 12, 2018
    Publication date: July 26, 2018
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Publication number: 20180187168
    Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array or zinc finger, and a catalytic domain comprising a sequence that catalyzes hydroxylation of methylated cytosines in DNA, and methods of use thereof.
    Type: Application
    Filed: February 12, 2018
    Publication date: July 5, 2018
    Inventors: J. Keith Joung, Morgan Maeder, James Angstman