Patents by Inventor J. Keith Joung

J. Keith Joung has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210309701
    Abstract: Drug-inducible, tunable, and multiplexable Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 (Cpf1)-based activators, and methods of use thereof.
    Type: Application
    Filed: April 23, 2018
    Publication date: October 7, 2021
    Inventors: Yu Gyoung Tak, Benjamin Kleinstiver, J. Keith Joung
  • Publication number: 20210292795
    Abstract: The present invention relates to methods to improve the absolute rate of homology-directed repair (HDR) and/or to improve the relative rate of HDR compared with non-homologous end joining (NHEJ).
    Type: Application
    Filed: May 26, 2021
    Publication date: September 23, 2021
    Inventors: J. Keith JOUNG, Benjamin KLEINSTIVER, Jason Michael GEHRKE, Shengdar TSAI, James ANGSTMAN, Rebecca Tayler COTTMAN
  • Publication number: 20210269788
    Abstract: Engineered CRISPR from Prevotella and Francisella 1 (Cpf1) nucleases with improved targeting range and enhanced on-target activity, and their use in genomic engineering, epigenomic engineering, base editing, genome targeting, genome editing, and in vitro diagnostics.
    Type: Application
    Filed: April 29, 2021
    Publication date: September 2, 2021
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Alexander Sousa
  • Patent number: 11098326
    Abstract: Many studies have shown that CRISPR-Cas nucleases can tolerate up to five mismatches and still cleave; it is hard to predict the effects of any given single or combination of mismatches on activity. Taken together, these nucleases can show significant off-target effects but it can be challenging to predict these sites. Described herein are methods for increasing the specificity of genome editing using the CRISPR/Cas system, e.g., using RNA-guided FokI Nucleases (RFNs), e.g., FokI-Cas9 or FokI-dCas9-based fusion proteins.
    Type: Grant
    Filed: January 24, 2020
    Date of Patent: August 24, 2021
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Shengdar Tsai
  • Patent number: 11060078
    Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.
    Type: Grant
    Filed: January 6, 2020
    Date of Patent: July 13, 2021
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Vikram Pattanayak
  • Patent number: 11041155
    Abstract: Described herein are engineered CCCTC-binding factor (CTCF) variants that can bind to mutant CTCF binding sequences and method of using the same.
    Type: Grant
    Filed: May 17, 2019
    Date of Patent: June 22, 2021
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Rebecca Tayler Cottman
  • Patent number: 11028429
    Abstract: Sensitive, unbiased methods for genome-wide detection of potential off-target nuclease cleavage sites in DNA, e.g., in cell type-specific genomic DNA samples.
    Type: Grant
    Filed: May 31, 2018
    Date of Patent: June 8, 2021
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Shengdar Tsai
  • Patent number: 11021718
    Abstract: The present invention relates to methods to improve the absolute rate of homology-directed repair (HDR) and/or to improve the relative rate of HDR compared with non-homologous end joining (NHEJ).
    Type: Grant
    Filed: October 1, 2015
    Date of Patent: June 1, 2021
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Benjamin Kleinstiver, Jason Michael Gehrke, Shengdar Tsai, James Angstman, Rebecca Tayler Cottman
  • Publication number: 20210155984
    Abstract: Methods and compositions for performing highly sensitive in vitro assays to define substrate preferences and off-target sites of nucleic-acid binding, modifying, and cleaving agents.
    Type: Application
    Filed: November 30, 2020
    Publication date: May 27, 2021
    Inventors: J. Keith Joung, Vikram Pattanayak, Karl Petri, Kanae Esther Sasaki
  • Publication number: 20210155907
    Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array or zinc finger, and a catalytic domain comprising a sequence that catalyzes hydroxylation of methylated cytosines in DNA, and methods of use thereof.
    Type: Application
    Filed: January 14, 2021
    Publication date: May 27, 2021
    Inventors: J. Keith Joung, Morgan Maeder, James Angstman
  • Publication number: 20210130850
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Application
    Filed: November 16, 2020
    Publication date: May 6, 2021
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Publication number: 20210102213
    Abstract: Described herein are engineered CCCTC-binding factor (CTCF) variants that can bind to mutant CTCF binding sequences and method of using the same.
    Type: Application
    Filed: December 10, 2020
    Publication date: April 8, 2021
    Inventors: Rebecca Tayler Cottman, J. Keith Joung
  • Publication number: 20210071248
    Abstract: Methods and compositions for performing highly sensitive in vitro assays to define substrate preferences and off-target sites of nucleic-acid binding, modifying, and cleaving agents.
    Type: Application
    Filed: April 17, 2020
    Publication date: March 11, 2021
    Inventors: J. Keith Joung, Vikram Pattanayak, Karl Petri
  • Patent number: 10894950
    Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array or zinc finger, and a catalytic domain comprising a sequence that catalyzes hydroxylation of methylated cytosines in DNA, and methods of use thereof.
    Type: Grant
    Filed: February 12, 2018
    Date of Patent: January 19, 2021
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Morgan Maeder, James Angstman
  • Publication number: 20200399620
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Application
    Filed: September 2, 2020
    Publication date: December 24, 2020
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Publication number: 20200399621
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Application
    Filed: September 4, 2020
    Publication date: December 24, 2020
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Patent number: 10844403
    Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: November 24, 2020
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, James Angstman, Jeffry D. Sander, Morgan Maeder, Shengdar Tsai
  • Publication number: 20200354704
    Abstract: Methods and constructs for RNA-guided targeting of transcriptional activators to specific genomic loci.
    Type: Application
    Filed: July 27, 2020
    Publication date: November 12, 2020
    Inventors: J. Keith Joung, Morgan Maeder
  • Patent number: 10808233
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: October 20, 2020
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Publication number: 20200308571
    Abstract: Engineered adenine base editor (ABE) variants with reduced RNA editing activity, and methods of using the same.
    Type: Application
    Filed: February 4, 2020
    Publication date: October 1, 2020
    Inventors: J. Keith Joung, Julian Grunewald, Ronghao Zhou