Patents by Inventor J. Michael Ramsey

J. Michael Ramsey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6822225
    Abstract: A method and apparatus is disclosed for flowing a sample gas and a reactant gas (38, 43) past a corona discharge electrode (26) situated at a first location in an ion drift chamber (24), applying a pulsed voltage waveform comprising a varying pulse component and a dc bias component to the corona discharge electrode (26) to cause a corona which in turn produces ions from the sample gas and the reactant gas, applying a dc bias to the ion drift chamber (24) to cause the ions to drift to a second location (25) in the ion drift chamber (24), detecting the ions at the second location (25) in the drift chamber (24), and timing the period for the ions to drift from the corona discharge electrode to the selected location in the drift chamber.
    Type: Grant
    Filed: September 25, 2002
    Date of Patent: November 23, 2004
    Assignee: UT-Battelle LLC
    Inventors: Jun Xu, J. Michael Ramsey, William B. Whitten
  • Publication number: 20040224397
    Abstract: Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.
    Type: Application
    Filed: June 1, 2004
    Publication date: November 11, 2004
    Inventors: Christopher T. Culbertson, Stephen C. Jacobson, Maxine A. McClain, J. Michael Ramsey
  • Publication number: 20040195099
    Abstract: Disclosed are microfluidic devices and methods for performing analyses which, in a preferred embodiment, involve sample filtration, solid phase extraction for enrichment of target analytes and separation of target analytes via open channel electrochromatography. Small sample volumes of mixed analytes are resolvable in less than one minute using the disclosed devices and methods.
    Type: Application
    Filed: April 4, 2003
    Publication date: October 7, 2004
    Inventors: Stephen C. Jacobson, J. Michael Ramsey
  • Patent number: 6790328
    Abstract: A microfluidic device for forming and/or dispensing minute volume segments of a material is described. In accordance with one aspect of the present invention, a microfluidic device and method is provided for spatially confining the material in a focusing element. The device is also capable of segmenting the confined material into minute volume segments, and dispensing a volume segment to a waste or collection channel. The device further includes means for driving the respective streams of sample and focusing fluids through respective channels into a chamber, such that the focusing fluid streams spatially confine the sample material. The device may also include additional means for driving a minute volume segment of the spatially confined sample material into a collection channel in fluid communication with the waste reservoir.
    Type: Grant
    Filed: January 12, 2001
    Date of Patent: September 14, 2004
    Assignee: UT-Battelle, LLC
    Inventors: Stephen C. Jacobson, J. Michael Ramsey
  • Patent number: 6783647
    Abstract: Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.
    Type: Grant
    Filed: October 19, 2001
    Date of Patent: August 31, 2004
    Assignee: UT-Battelle, LLC
    Inventors: Christopher T. Culbertson, Stephen C. Jacobson, Maxine A. McClain, J. Michael Ramsey
  • Publication number: 20040164238
    Abstract: A method and apparatus is disclosed for flowing a sample gas and a reactant gas (38, 43) past a corona discharge electrode (26) situated at a first location in an ion drift chamber (24), applying a pulsed voltage waveform comprising a varying pulse component and a dc bias component to the corona discharge electrode (26) to cause a corona which in turn produces ions from the sample gas and the reactant gas, applying a dc bias to the ion drift chamber (24) to cause the ions to drift to a second location (25) in the ion drift chamber (24), detecting the ions at the second location (25) in the drift chamber (24), and timing the period for the ions to drift from the corona discharge electrode to the selected location in the drift chamber.
    Type: Application
    Filed: September 25, 2002
    Publication date: August 26, 2004
    Inventors: Jun Xu, J. Michael Ramsey, William B. Whitten
  • Publication number: 20040144648
    Abstract: A microfluidic device and method for forming and dispensing minute volume segments of a material are described. In accordance with the present invention, a microfluidic device and method are provided for spatially confining the material in a focusing element. The device is also adapted for segmenting the confined material into minute volume segments, and dispensing a volume segment to a waste or collection channel. The device further includes means for driving the respective streams of sample and focusing fluids through respective channels into a chamber, such that the focusing fluid streams spatially confine the sample material. The device may also include additional means for driving a minute volume segment of the spatially confined sample material into a collection channel in fluid communication with the waste reservoir.
    Type: Application
    Filed: January 16, 2004
    Publication date: July 29, 2004
    Inventors: Stephen C. Jacobson, J. Michael Ramsey
  • Publication number: 20040137445
    Abstract: A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip is fabricated using standard photolithographic procedures chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.
    Type: Application
    Filed: May 9, 2003
    Publication date: July 15, 2004
    Inventor: J. Michael Ramsey
  • Patent number: 6685809
    Abstract: A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microchannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
    Type: Grant
    Filed: February 4, 1999
    Date of Patent: February 3, 2004
    Assignee: UT-Battelle, LLC
    Inventors: Stephen C. Jacobson, J. Michael Ramsey, Christopher T. Culbertson, William B. Whitten, Robert S. Foote
  • Publication number: 20040007464
    Abstract: A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.
    Type: Application
    Filed: May 9, 2003
    Publication date: January 15, 2004
    Inventor: J. Michael Ramsey
  • Publication number: 20040009517
    Abstract: A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip fabricated using standard photolithographic procedures chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.
    Type: Application
    Filed: May 9, 2003
    Publication date: January 15, 2004
    Inventor: J. Michael Ramsey
  • Publication number: 20040007463
    Abstract: A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
    Type: Application
    Filed: February 24, 2003
    Publication date: January 15, 2004
    Inventors: J. Michael Ramsey, Stephen C. Jacobson
  • Publication number: 20030226755
    Abstract: A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.
    Type: Application
    Filed: April 30, 2003
    Publication date: December 11, 2003
    Inventor: J. Michael Ramsey
  • Publication number: 20030226753
    Abstract: A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary, electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.
    Type: Application
    Filed: April 30, 2003
    Publication date: December 11, 2003
    Inventor: J. Michael Ramsey
  • Patent number: 6660480
    Abstract: A method and apparatus for analyzing nucleic acids includes immobilizing nucleic probes at specific sites within a microchannel structure and moving target nucleic acids into proximity to the probes in order to allow hybridization and fluorescence detection of specific target sequences.
    Type: Grant
    Filed: March 5, 2002
    Date of Patent: December 9, 2003
    Assignee: UT-Battelle, LLC
    Inventors: J. Michael Ramsey, Robert S. Foote
  • Publication number: 20030205470
    Abstract: A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.
    Type: Application
    Filed: April 30, 2003
    Publication date: November 6, 2003
    Inventor: J. Michael Ramsey
  • Publication number: 20030205469
    Abstract: A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.
    Type: Application
    Filed: April 30, 2003
    Publication date: November 6, 2003
    Inventor: J. Michael Ramsey
  • Publication number: 20030150733
    Abstract: A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.
    Type: Application
    Filed: October 1, 2002
    Publication date: August 14, 2003
    Inventor: J. Michael Ramsey
  • Publication number: 20030086333
    Abstract: A device for electrohydrodynamically (EHD) mixing fluids includes a mixing channel, the mixing channel having at least one supply channel fluidicly connected thereto for transport of fluid into the mixing channel. At least two electrodes are provided, at least one of the electrodes for charging at least a portion of the fluid in the mixing channel. The electrodes impose an electric field in the mixing channel to induce EHD mixing of the fluid in the mixing channel. A method for EHD mixing of fluids applies an electric field to a mixing channel to induce EHD mixing.
    Type: Application
    Filed: November 5, 2001
    Publication date: May 8, 2003
    Inventors: Constantinos Tsouris, David W. DePaoli, Christopher T. Culbertson, Stephen C. Jacobson, J. Michael Ramsey
  • Publication number: 20030075446
    Abstract: Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.
    Type: Application
    Filed: October 19, 2001
    Publication date: April 24, 2003
    Inventors: Christopher T. Culbertson, Stephen C. Jacobson, Maxine A. McClain, J. Michael Ramsey