Abstract: A method for fabricating a cornea includes affixing a frame to at least one cell culture insert comprising a generally cylindrical structure having a proximal end and a distal end, a base disposed at the proximal end, and a porous membrane disposed between the proximal end and the distal end; affixing a dome-shaped member to the porous membrane within the frame, the dome-shaped member comprising a crown, a dome base, and a surface connecting the crown and the dome base; depositing a material comprising a matrix-forming compound on the frame such that the crown and at least a portion of the surface of the dome-shaped member is coated with the material comprising the matrix-forming compound; and removing the dome-shaped member to produce a fabricated cornea attached to the frame. A system for fabricating a cornea and a cornea scaffold are also described herein.

Abstract: A method for fabricating a cornea includes affixing a frame to at least one cell culture insert comprising a generally cylindrical structure having a proximal end and a distal end, a base disposed at the proximal end, and a porous membrane disposed between the proximal end and the distal end; affixing a dome-shaped member to the porous membrane within the frame, the dome-shaped member comprising a crown, a dome base, and a surface connecting the crown and the dome base; depositing a material comprising a matrix-forming compound on the frame such that the crown and at least a portion of the surface of the dome-shaped member is coated with the material comprising the matrix-forming compound; and removing the dome-shaped member to produce a fabricated cornea attached to the frame. A system for fabricating a cornea and a cornea scaffold are also described herein.

Abstract: Methods and systems to isolate microvessels using an enriched or purified enzyme to dissociate tissue are described. The systems and methods include a second digestion to digest a top layer, from a first digestion and first centrifuge operation, with the enriched or purified enzyme to generate a second fat-enzyme solution, a second centrifuge operation, and isolation of the microvessels from pellets generated by the first and second centrifuge operations. The systems and methods may include washing the second fat-enzyme solution with an enzyme inhibitor in a post-digestion wash.

Abstract: Systems and methods are disclosed for machine classifiers that employ enhanced machine learning. The machine classification may be automated, based on the input of human classifiers, or a combination of both. The selection of human classifiers is determined by a classifier ranking or scoring process. In addition, data generated by the ranking or scoring process can be used to train the machine classifiers to more accurately classify data.

Abstract: The present disclosure relates to compositions and methods for the treatment of blood vessel disease. More specifically, the compositions and methods of the present disclosure make use of a population of stromal vascular fraction cells. In some embodiments, the population of stromal vascular fraction cells comprises at least one macrophage.

Abstract: Systems and methods are disclosed for machine classifiers that employ enhanced machine learning. The machine classification may be automated, based on the input of human classifiers, or a combination of both. The selection of human classifiers is determined by a classifier ranking or scoring process. In addition, data generated by the ranking or scoring process can be used to train the machine classifiers to more accurately classify data.

Abstract: The present invention provides greetings card or similar flat pack folding sheet self-supporting display article that comprises: at least one sheet that is flat in an initial state but when folded to its operational configuration defines a rocking base that has a platform or summit supported by at least one rocker and the article having an upstanding card portion or panel serving as a display panel bearing text or graphic images that extends upright through the platform or summit.

Abstract: Microtubules are excellent candidates for the fabrication of nanostructures, including nanowires. A method for controlled nucleation and growth of microtubules on substrates (e.g., gold on a silicon wafer) is provided. The substrate is functionalized with a nucleating agent for microtubule growth. The method can be employed to generate nanoscale structures on substrates or between substrates by additional attachment of MT capture agents which function to capture the ends of growing MT to form connecting MT structures. The method can be used to form 2- and 3-D structures on or between substrates and can function to establish interconnects between nanoscale devices or molecular electronic devices and electrodes. A specific method for metallization of biological macromolecules and structures in provides which can be beneficially applied to metallized the MT formed by the growth and capture method. The metallization method is biologically benign and is particularly useful for copper metallization of MTs.

Abstract: This application discloses methods and materials for preparing functional microvascular beds in the laboratory. These prevascularized constructs can be used to vascularize engineered tissue constructs or to revascularize damaged or diseased tissues or organs following implantation. The prevascularized constructs may also deliver genetically engineered gene products to the bloodstream.