Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: A flow-through pressure regulator includes a retainer that secures a diaphragm relative to a seat, and includes an intermediate portion, an end portion and a base portion. The intermediate portion extends along a longitudinal axis and is fixed with respect to the seat. The end portion extends from the intermediate portion and includes a concave surface facing the seat. The regulator includes multiple flow diffusers such that the end portion includes at least one aperture having a flow axis that is oblique to the longitudinal axis so as to reduce noise due to fluid flow and/or the seat forms a passage having an increasing cross-sectional area from an inlet to an outlet.

Abstract: A filter module for an automotive fuel supply system includes a housing, a filter mounted in the housing, a pressure regulator conduit mounted in the housing, and a pressure regulator. The housing includes a module inlet and a module outlet. The filter is in fluid communication with the module inlet and the module outlet. The pressure regulator conduit includes a regulator inlet in fluid communication with the filter, regulator outlet, and a receptacle in fluid communication with the regulator inlet and the regulator outlet. The pressure regulator extends into the void between the first surface of the receptacle and the second surface of the receptacle.

Abstract: An automatic pump testing device is configured with controller, a pump testing station, a supply tank and a collection tank. In accordance with one aspect of the present invention, a pump testing device is configured to test various types of hand actuated pumps. In accordance with another aspect of the present invention, the pump testing device is configured to test various end of life modes, length of life, and other pump performance information, including pump performance information measured over time.

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: A pressure regulation assembly of a fuel supply includes a receptacle, an inlet connectable to the fuel supply and in fluid communication with the receptacle, an outlet in fluid communication with the receptacle, and a pressure regulator mounted in the receptacle to selectively open fluid communication between the inlet and the outlet. The receptacle includes a first surface surrounding an axis, and a second surface surrounding the axis and the first surface to provide a void between the first surface and the second surface. The pressure regulator extends into the void between the first surface of the receptacle and the second surface of the receptacle and includes a third surface surrounding the axis and a fourth surface surrounding the axis and the third surface. A first seal engages the first surface of the receptacle and the third surface of the pressure regulator. A second seal engages the second surface of the receptacle and the fourth surface of the pressure regulator.

Abstract: A pump module for mounting in a fuel tank of an automotive fuel supply system includes a housing, a fuel pump mounted in the housing, a pressure regulator conduit mounted in the housing, and a pressure regulator. The housing includes a module inlet and a module outlet in fluid communication with the module inlet. The fuel pump is in fluid communication with the module inlet. The pressure regulator conduit is in fluid communication with the fuel pump. The pressure regulator conduit includes a regulator inlet, regulator outlet, and a receptacle in fluid communication with the regulator inlet and the regulator outlet. The receptacle includes a first surface surrounding an axis, and a second surface surrounding the axis and the first surface to provide a void between the first surface and the second surface. The pressure regulator is mounted in the receptacle to selectively open fluid communication between the regulator inlet and the regulator outlet.

Abstract: A pump module for mounting in a fuel tank of an automotive fuel supply system includes a housing, a fuel pump mounted in the housing, a pressure regulator conduit mounted in the housing, and a pressure regulator. The housing includes a module inlet and a module outlet in fluid communication with the module inlet. The fuel pump is in fluid communication with the module inlet. The pressure regulator conduit is in fluid communication with the fuel pump. The pressure regulator conduit includes a regulator inlet, regulator outlet, and a receptacle in fluid communication with the regulator inlet and the regulator outlet. The receptacle includes a first surface surrounding an axis, and a second surface surrounding the axis and the first surface to provide a void between the first surface and the second surface. The pressure regulator is mounted in the receptacle to selectively open fluid communication between the regulator inlet and the regulator outlet.

Abstract: A filter module for an automotive fuel supply system includes a housing, a filter mounted in the housing, a pressure regulator conduit mounted in the housing, and a pressure regulator. The housing includes a module inlet and a module outlet. The filter is in fluid communication with the module inlet and the module outlet. The pressure regulator conduit includes a regulator inlet in fluid communication with the filter, regulator outlet, and a receptacle in fluid communication with the regulator inlet and the regulator outlet. The receptacle includes a first surface surrounding an axis, and a second surface surrounding the axis and the first surface to provide a void between the first surface and the second surface. The pressure regulator is mounted in the receptacle to selectively open fluid communication between the regulator inlet and the regulator outlet. The pressure regulator extends into the void between the first surface of the receptacle and the second surface of the receptacle.

Abstract: A flow-through pressure regulator includes a retainer that secures a diaphragm relative to a seat, and includes an intermediate portion, an end portion and a base portion. The intermediate portion extends along a longitudinal axis and is fixed with respect to the seat. The end portion extends from the intermediate portion and includes a concave surface facing the seat. The regulator includes multiple flow diffusers such that the end portion includes at least one aperture having a flow axis that is oblique to the longitudinal axis so as to reduce noise due to fluid flow and/or the seat forms a passage having an increasing cross-sectional area from an inlet to an outlet.

Abstract: A pressure regulation assembly of a fuel supply includes a receptacle, an inlet connectable to the fuel supply and in fluid communication with the receptacle, an outlet in fluid communication with the receptacle, and a pressure regulator mounted in the receptacle to selectively open fluid communication between the inlet and the outlet. The receptacle includes a first surface surrounding an axis, and a second surface surrounding the axis and the first surface to provide a void between the first surface and the second surface. The pressure regulator extends into the void between the first surface of the receptacle and the second surface of the receptacle and includes a third surface surrounding the axis and a fourth surface surrounding the axis and the third surface. A first seal engages the first surface of the receptacle and the third surface of the pressure regulator. A second seal engages the second surface of the receptacle and the fourth surface of the pressure regulator.

Abstract: A fuel component assembly having a fuel component disposed between a base and a cover, which prevent fluid communication to an exterior thereof. The fuel component defines at least one aperture, the base supports the fuel component and defines a passage in communication with the at least one aperture. The cover is located proximate the base. A securement having a projection and the receiver that engage to form a fluid tight seal. The projection is radially disposed about a longitudinal axis of the fuel component, and the receiver continuously engages the projection. The projection being disposed on one of the cover and the base, and the receiver being disposed on the other of the cover and the base. The fuel component assembly allows for a method of encapsulating a fuel component within a fuel supply.

Abstract: A fuel component assembly having a fuel component disposed between a base and a cover, which prevent fluid communication to an exterior thereof. The fuel component defines at least one aperture, the base supports the fuel component and defines a passage in communication with the at least one aperture. The cover is located proximate the base. A securement having a projection and the receiver that engage to form a fluid tight seal. The projection is radially disposed about a longitudinal axis of the fuel component, and the receiver continuously engages the projection. The projection being disposed on one of the cover and the base, and the receiver being disposed on the other of the cover and the base. The fuel component assembly allows for a method of encapsulating a fuel component within a fuel supply.

Abstract: A fuel component assembly having a fuel component disposed between a base and a cover, which prevent fluid communication to an exterior thereof. The fuel component defines at least one aperture, the base supports the fuel component and defines a passage in communication with the at least one aperture. The cover is located proximate the base. A securement having a projection and the receiver that engage to form a fluid tight seal. The projection is radially disposed about a longitudinal axis of the fuel component, and the receiver continuously engages the projection. The projection being disposed on one of the cover and the base, and the receiver being disposed on the other of the cover and the base. The fuel component assembly allows for a method of encapsulating a fuel component within a fuel supply.

Abstract: An internal fuel filter for a pressure regulator having a body that encloses a fuel flow path. A first support includes a first surface adapted to be sealingly surrounded by the body. A second support is spaced from the first support along an axis. At least one rib is disposed between and contiguous with the first and second supports, wherein one of the rib and the second support includes a second surface adapted to sealingly surrounded the body. A filter element extends between the first support and the second support and surrounds the axis.

Abstract: A fuel component assembly having a fuel component disposed between a base and a cover, which prevent fluid communication to an exterior thereof. The fuel component defines at least one aperture, the base supports the fuel component and defines a passage in communication with the at least one aperture. The cover is located proximate the base. A securement having a projection and the receiver that engage to form a fluid tight seal. The projection is radially disposed about a longitudinal axis of the fuel component, and the receiver continuously engages the projection. The projection being disposed on one of the cover and the base, and the receiver being disposed on the other of the cover and the base. The fuel component assembly allows for a method of encapsulating a fuel component within a fuel supply.

Abstract: A connector assembly and a method of disconnecting the connector assembly. The connector assembly comprises a first part and a second part. The first part extends along a longitudinal axis and has a protrusion that extends radially outward from the longitudinal axis. The second part receives the first part and has a wall surrounding the longitudinal axis. The wall has an inner surface generally confronting the longitudinal axis, an outer surface generally facing opposite the inner surface, a channel cooperatively receiving the protrusion; and an arm portion. The arm portion has a first surface defining a portion of the inner surface, a second surface defining a portion of the outer surface, and a bulge extending from the arm portion into the channel. The bulge engages the protrusion and maintains the first and second parts in a connected configuration.

Abstract: A connector assembly and a method of disconnecting the connector assembly. The connector assembly comprises a first part and a second part. The first part extends along a longitudinal axis and has a protrusion that extends radially outward from the longitudinal axis. The second part receives the first part and has a wall surrounding the longitudinal axis. The wall has an inner surface generally confronting the longitudinal axis, an outer surface generally facing opposite the inner surface, a channel cooperatively receiving the protrusion; and an arm portion. The arm portion has a first surface defining a portion of the inner surface, a second surface defining a portion of the outer surface, and a bulge extending from the arm portion into the channel. The bulge engages the protrusion and maintains the first and second parts in a connected configuration.

Abstract: An internal fuel filter for a pressure regulator having a body that encloses a fuel flow path. A first support includes a first surface adapted to be sealingly surrounded by the body. A second support is spaced from the first support along an axis. At least one rib is disposed between and contiguous with the first and second supports, wherein one of the rib and the second support includes a second surface adapted to sealingly surrounded the body. A filter element extends between the first support and the second support and surrounds the axis.

Abstract: The fuel filter/regulator assembly comprises a cylindrical body open at one end and closed at its opposite end by an annular wall having a pocket for receiving the regulator. The annular fuel filter is received in the body and has an inner end cap configured to bear against and receive an upper portion of the regulator. A further end cap closes the open end of the body and bears against the fuel filter element. A fuel inlet is provided and all components of the housing are formed of stainless steel. In another form, a wave spring is employed to accommodate stack-up tolerances of the fuel filter.