Patents by Inventor Jarrod Provins

Jarrod Provins has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240218438
    Abstract: Disclosed herein is a method of causing enzymatic digestion of in vitro synthesised nucleic acid (especially DNA), the method comprising the steps of: (a) combining reagents, in the presence of a temporarily substantially inactive nuclease, to form in vitro synthesised nucleic acid; and (b) subsequently permitting or causing the substantially inactive nuclease to regain substantial nuclease activity after a period of time has elapsed sufficient to allow detection of the in vitro synthesised nucleic acid, such that the in vitro synthesised nucleic acid is digested by the nuclease.
    Type: Application
    Filed: May 27, 2022
    Publication date: July 4, 2024
    Inventors: Victor Perez, Alexis Quintilliani, Bryan Kraynack, Daiwei Shen, Jarrod Provins
  • Publication number: 20240026433
    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) using a nicking enzyme to cause a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands to as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to shu
    Type: Application
    Filed: April 20, 2023
    Publication date: January 25, 2024
    Inventors: Daiwei Shen, Bryan Kraynack, Victor Perez, Jarrod Provins
  • Publication number: 20230340579
    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) causing a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands so as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to a reduction of at least 2°
    Type: Application
    Filed: January 26, 2023
    Publication date: October 26, 2023
    Inventors: Jarrod Provins, Daiwei Shen, Bryan Kraynack
  • Patent number: 11655496
    Abstract: A method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridization event in which the primers hybridize to the target, which hybridization event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) using a nicking enzyme to cause a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands so as to form newly synthesized nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesized nucleic acid.
    Type: Grant
    Filed: January 2, 2019
    Date of Patent: May 23, 2023
    Assignee: LumiraDx UK Ltd.
    Inventors: Daiwei Shen, Bryan Kraynack, Victor Perez, Jarrod Provins
  • Patent number: 11591643
    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, wherein the temperature at which the method is performed is non-isothermal and subject to a reduction of at least 2° C. during amplification process steps. The present invention provides an improved nucleic acid amplification technique having one or more advantages over existing techniques including, for example, decreased reaction time, increased yield, and decreased non-specific amplification products.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 28, 2023
    Assignee: LumiraDx UK Ltd.
    Inventors: Jarrod Provins, Daiwei Shen, Bryan Kraynack
  • Publication number: 20220403459
    Abstract: Disclosed is a composition comprising a nicking enzyme and a water-soluble rubidium salt, and a method of performing a reaction catalysed by a nicking enzyme including the presence of a water-soluble rubidium salt in the reaction.
    Type: Application
    Filed: June 9, 2020
    Publication date: December 22, 2022
    Inventors: Jarrod PROVINS, Victor PEREZ, Daiwei SHEN, Anthony FARINA, Bryan KRAYNACK
  • Publication number: 20210388425
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Application
    Filed: August 11, 2021
    Publication date: December 16, 2021
    Inventors: Brian K. Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Publication number: 20210292826
    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) using a nicking enzyme to cause a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands to as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to shu
    Type: Application
    Filed: January 2, 2019
    Publication date: September 23, 2021
    Applicant: LUMIRADX UK LTD
    Inventors: Daiwei SHEN, Bryan KRAYNACK, Victor PEREZ, Jarrod PROVINS
  • Publication number: 20210155965
    Abstract: A method includes combining a polynucleotide and an amplification reagent mixture to form a reaction mixture, wherein the reaction mixture comprises reversibly bound divalent ions in solution, and adjusting the pH of the reaction mixture to release the reversibly bound divalent ions, thereby initiating amplification of the polynucleotide.
    Type: Application
    Filed: December 3, 2020
    Publication date: May 27, 2021
    Inventors: Honghua Zhang, Jarrod Provins, Richard Roth
  • Patent number: 10927393
    Abstract: A method includes combining a polynucleotide and an amplification reagent mixture to form a reaction mixture, wherein the reaction mixture comprises reversibly bound divalent ions in solution, and adjusting the pH of the reaction mixture to release the reversibly bound divalent ions, thereby initiating amplification of the polynucleotide.
    Type: Grant
    Filed: June 7, 2013
    Date of Patent: February 23, 2021
    Assignee: Ionian Technologies, LLC
    Inventors: Honghua Zhang, Jarrod Provins, Richard Roth
  • Patent number: 10851406
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: December 1, 2020
    Assignee: Ionian Technologies, LLC
    Inventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Publication number: 20190323072
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Application
    Filed: January 9, 2019
    Publication date: October 24, 2019
    Inventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Publication number: 20190226015
    Abstract: Disclosed is a method of performing a non-isothermal nucleic acid amplification reaction, the method comprising the steps of: (a) mixing a target sequence with one or more complementary single stranded primers in conditions which permit a hybridisation event in which the primers hybridise to the target, which hybridisation event, directly or indirectly, leads to the formation of a duplex structure comprising two nicking sites disposed at or near opposite ends of the duplex; and performing an amplification process by; (b) causing a nick at each of said nicking sites in the strands of the duplex; (c) using a polymerase to extend the nicked strands so as to form newly synthesised nucleic acid, which extension with the polymerase recreates nicking sites; (d) repeating steps (b) and (c) as desired so as to cause the production of multiple copies of the newly synthesised nucleic acid; characterised in that the temperature at which the method is performed is non-isothermal, and subject to a reduction of at least 2°
    Type: Application
    Filed: June 30, 2017
    Publication date: July 25, 2019
    Applicant: LUMIRADX UK LTD
    Inventors: Jarrod PROVINS, Daiwei SHEN, Bryan KRAYNACK
  • Publication number: 20180023118
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Application
    Filed: May 22, 2017
    Publication date: January 25, 2018
    Inventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Publication number: 20180023130
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Application
    Filed: March 23, 2017
    Publication date: January 25, 2018
    Inventors: Brian K. Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Patent number: 9617586
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Grant
    Filed: July 14, 2008
    Date of Patent: April 11, 2017
    Assignee: Ionian Technologies, Inc.
    Inventors: Brian K. Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard B. Roth, Jeffrey Mandell
  • Patent number: 9562264
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: February 7, 2017
    Assignee: Ionian Technologies, Inc.
    Inventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Patent number: 9562263
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: February 7, 2017
    Assignee: Ionian Technologies, Inc.
    Inventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Publication number: 20140093883
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Application
    Filed: October 30, 2013
    Publication date: April 3, 2014
    Applicant: Ionian Technologies, Inc.
    Inventors: Brian K. Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
  • Publication number: 20140072978
    Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.
    Type: Application
    Filed: October 30, 2013
    Publication date: March 13, 2014
    Applicant: Ionian Technologies, Inc.
    Inventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell