Patents by Inventor Jason O. Fiering

Jason O. Fiering has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190307946
    Abstract: A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive comprising a cell activator, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel to accumulate target cells in a primary stream and non-target cells in a secondary stream. A system for microfluidic cell separation capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive comprising a cell activator, and at least one acoustic transducer coupled to the microfluidic separation channel.
    Type: Application
    Filed: June 25, 2019
    Publication date: October 10, 2019
    Inventors: Jason O. Fiering, Kenneth T. Kotz, Nathan Francis Moore
  • Publication number: 20190290829
    Abstract: A method for separating cells in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
    Type: Application
    Filed: April 28, 2017
    Publication date: September 26, 2019
    Inventors: Jason O. Fiering, Kenneth T. Kotz
  • Patent number: 10232336
    Abstract: The systems and methods described herein relate to a high-throughput flow apparatus. The apparatus is used with an array of wells, and is configured to impart a predetermined shear stress on cells cultured within each of the wells of the array of wells. The apparatus includes a plurality of mechanical tips. The plurality of mechanical tips each includes a head with a hemispheroid shape. The apparatus also includes a motor associated with at least one of plurality of mechanical tips. The motor is configured to drive the plurality of mechanical tips to impart the shear stress pattern in each of the wells.
    Type: Grant
    Filed: November 6, 2014
    Date of Patent: March 19, 2019
    Assignees: The Charles Stark Draper Laboratory, Inc., The Brigham and Women's Hospital, Inc.
    Inventors: Guillermo Garcia-Cardena, Peter Mack, Jeffrey T. Borenstein, Ahmad S. Khalil, Eli J. Weinberg, Jason O. Fiering, Ernest S. Kim, William J. Adams, Jr., Mitchell Hansberry, Stephen Bellio
  • Patent number: 10166323
    Abstract: Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: January 1, 2019
    Assignee: The Charles Stark Draper Laboratories, Inc.
    Inventors: Jason O. Fiering, Shivshanker Sundaram, Andrew Meuller
  • Publication number: 20180362908
    Abstract: Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.
    Type: Application
    Filed: August 22, 2018
    Publication date: December 20, 2018
    Inventors: Jeffrey T. Borenstein, Joseph L. Charest, Christopher M. DiBiasio, Dorit Berlin, Jenna Balestrini, Jose A. Santos, Vishal Tandon, Jason O. Fiering
  • Publication number: 20180313816
    Abstract: A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
    Type: Application
    Filed: April 27, 2018
    Publication date: November 1, 2018
    Inventors: Jason O. Fiering, Kenneth T. Kotz
  • Patent number: 9974898
    Abstract: Systems, methods, and compositions for removing organophosphate toxins from blood are disclosed herein. The compositions include a lipid-based capture particle that displays BChE that binds to the toxin. The methods include acoustically separating toxins bound to lipid-based capture particles from blood factors of whole blood.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: May 22, 2018
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventors: Tammy Spain, Jason O. Fiering, Leila Albers
  • Publication number: 20180066219
    Abstract: Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.
    Type: Application
    Filed: September 14, 2017
    Publication date: March 8, 2018
    Inventors: Jeffrey T. Borenstein, Joseph L. Charest, Christopher M. DiBiasio, Dorit Berlin, Jenna Balestrini, Jose A. Santos, Vishal Tandon, Jason O. Fiering
  • Publication number: 20170298456
    Abstract: The system described herein for bacterial identification can be used as a point-of-care or lab-based diagnostic system. In some implementations, the system can be used to detect other foreign agents within blood or other samples. The system can include disposable microfluidic cartridges that are removable from the system. The microfluidic cartridges can receive a sample, such as a blood sample, that is suspected of containing bacterial cells and separate the bacterial cells from the blood sample. Once the bacterial cells are separated from the blood, the system can introduce the recombinant detector bacteriophages into the system that can infect the bacterial cells. The system can then detect the expression of reporter genes from the recombinant detector bacteriophages.
    Type: Application
    Filed: March 27, 2017
    Publication date: October 19, 2017
    Applicant: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jason W. Holder, Jason O. Fiering
  • Patent number: 9764121
    Abstract: An implantable drug delivery apparatus for delivering a drug into a bodily fluid in a bodily cavity of a patient over a period of time includes a drug supply reservoir to supply drug into a delivery channel and an actuator for delivering the drug to a predetermined location in the bodily cavity of the patient, such as, for example, a cochlea of a human ear. The drug is loaded into the delivery channel while producing substantially negligible flow at an outlet of the delivery channel.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: September 19, 2017
    Assignees: THE CHARLES STARK DRAPER LABORATORY, INC., MASSACHUSETTS EYE AND EAR INFIRMARY
    Inventors: Jason O. Fiering, Mark J. Mescher, Erin E. Pararas, Jeffrey T. Borenstein, William F. Sewell, Sharon G. Kujawa, Michael J. McKenna, Ernest S. Kim
  • Patent number: 9748035
    Abstract: A method of forming a planar, low loss electrical component such as an inductor or transmission line is provided. A channel can be formed on a top surface of a substrate. A threading plate can be positioned on an upper surface of the channel. A wire or fiber can be introduced through the substrate, the channel, and the threading plate. The wire or fiber can then be guided into the channel using the threading plate. The substrate and the threading plate can then be removed.
    Type: Grant
    Filed: September 10, 2014
    Date of Patent: August 29, 2017
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventors: Amy Duwel, Joshua Nation, Jason O. Fiering, Doug White
  • Patent number: 9731062
    Abstract: Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.
    Type: Grant
    Filed: August 29, 2012
    Date of Patent: August 15, 2017
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventors: Jason O. Fiering, Shivshankar Sundaram
  • Publication number: 20170136168
    Abstract: Systems, methods, and compositions for removing organophosphate toxins from blood are disclosed herein. The compositions include a lipid-based capture particle that displays BChE that binds to the toxin. The methods include acoustically separating toxins bound to lipid-based capture particles from blood factors of whole blood.
    Type: Application
    Filed: November 28, 2016
    Publication date: May 18, 2017
    Inventors: Tammy Spain, Jason O. Fiering, Leila Albers
  • Patent number: 9651166
    Abstract: A microfluidic valve system includes a substrate, a valve seat a compliant membrane and a mechanically actuable displacement element. The substrate includes first and second channels embedded within it and includes a first layer of material and a second layer of material. The valve seat is in fluid communication with the first and second channels. Portions of the second layer of material form sidewalls of the second channel and the valve seat. The mechanically actuable displacement element applies a mechanical force to the compliant membrane to bring the compliant membrane into sealable contact with the valve seat, thereby closing the valve system.
    Type: Grant
    Filed: February 26, 2015
    Date of Patent: May 16, 2017
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Mark J. Mescher, Jason O. Fiering, Erin E. Swan
  • Patent number: 9645060
    Abstract: Devices and methods are disclosed herein for separating a supernate from a suspension. The apparatus consists of a sample zone, a controllable gate, and an analysis zone. The sample zone holds the suspension. The analysis zone passively transports a supernate formed from the suspension by capillary transport. A controllable gate prevents the suspension in the sample zone from flowing into the analysis zone. The controllable gate can be triggered after the supernate has separated from the suspension to allow the supernate to flow into the analysis zone.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: May 9, 2017
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventor: Jason O. Fiering
  • Patent number: 9504780
    Abstract: Systems, methods, and compositions for removing organophosphate toxins from blood are disclosed herein. The compositions include a lipid-based capture particle that displays BChE that binds to the toxin. The methods include acoustically separating toxins bound to lipid-based capture particles from blood factors of whole blood.
    Type: Grant
    Filed: January 30, 2014
    Date of Patent: November 29, 2016
    Assignee: THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventors: Tammy Spain, Jason O. Fiering, Leila Albers
  • Patent number: 9377422
    Abstract: Systems and methods are disclosed herein for a microfluidic calorimeter apparatus. A microfluidic calorimeter system includes a calorimetry apparatus and a processor in connection with the apparatus. The apparatus includes a microfluidic laminar flow channel connected to two inlets for flowing fluid into the laminar flow channel. Below the laminar flow channel is a plurality of microscale temperature sensors at known positions in the channel. The processor is in connection with the discrete temperature sensors and determines a calorimetry measurement based on local temperatures derived from data output by the microscale temperature sensors and the respective positions of the sensors in the channel.
    Type: Grant
    Filed: January 13, 2012
    Date of Patent: June 28, 2016
    Assignees: The Charles Stark Draper Laboratory, Inc., Northeastern University
    Inventors: Jason O. Fiering, Dale Larson, Gregory Kowalski, Mehmet Sen
  • Patent number: 9293821
    Abstract: In various embodiments, an actuating mechanism is employed to displace a conductor disposed within a fluidic channel, thereby reconfiguring an electronic component.
    Type: Grant
    Filed: July 8, 2010
    Date of Patent: March 22, 2016
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Amy E. Duwel, Jason O. Fiering, Douglas W. White, Mark J. Mescher, Joseph M. Bauer, John R. Lachapelle, Bryan Mclaughlin, Frans Spaepen
  • Publication number: 20160008532
    Abstract: Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.
    Type: Application
    Filed: March 10, 2014
    Publication date: January 14, 2016
    Inventors: Jason O. Fiering, Shivshanker Sundaram, Andrew Meuller
  • Publication number: 20160003229
    Abstract: A variable, closed-loop apparatus for regulating a microfluidic flow that employs a low-power deflection assembly, which is surface-mounted over a flexible membrane overlying a chamber integrated into a microfabricated platform. A flexible membrane, movable between two positions, sealingly overlies the chamber. One of the positions of the membrane restricts the flow through the chamber to a greater degree than the other position. A deflection assembly disposed on the substrate over the membrane unidirectionally deflects the membrane, thereby regulating the flow through the chamber.
    Type: Application
    Filed: July 9, 2015
    Publication date: January 7, 2016
    Inventors: Mark J. Mescher, Jason O. Fiering, Christopher E. Dube