Patents by Inventor Jay D. Keasling

Jay D. Keasling has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Genetically modified host cells and use of same for producing isoprenoid compounds
    Patent number: 9809829
    Abstract: The present invention provides genetically modified eukaryotic host cells that produce isoprenoid precursors or isoprenoid compounds. A subject genetically modified host cell comprises increased activity levels of one or more of mevalonate pathway enzymes, increased levels of prenyltransferase activity, and decreased levels of squalene synthase activity. Methods are provided for the production of an isoprenoid compound or an isoprenoid precursor in a subject genetically modified eukaryotic host cell. The methods generally involve culturing a subject genetically modified host cell under conditions that promote production of high levels of an isoprenoid or isoprenoid precursor compound.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: November 7, 2017
    Assignee: The Regents of the University of California
    Inventors: Jay D. Keasling, James Kirby, Eric M. Paradise
  • PRODUCTION OF FATTY ACIDS AND DERIVATIVES THEREOF
    Publication number: 20170275651
    Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.
    Type: Application
    Filed: March 7, 2017
    Publication date: September 28, 2017
    Inventors: Jay D. Keasling, Zhihao Hu, Chris Somerville, George Church, David Berry, Lisa C. Friedman, Andreas Schirmer, Shane Brubaker, Stephen B. DEL CARDAYRE
  • COMPOSITIONS AND METHODS FOR SINGLE-MOLECULE CONSTRUCTION OF DNA
    Publication number: 20170218416
    Abstract: The present invention provides for compositions and methods for single-molecule construction of DNA. The present invention provides for a method comprising: (a) providing a reaction chamber comprising a solid support bound to a single starter double-stranded (ds) DNA molecule comprising a free end, (b) introducing one or more extension molecules and one or more enzymes capable of joining a payload region of an extension molecule to the free end of starter dsDNA molecule to the reaction chamber wherein the extension molecule comprises an cleavable linker.
    Type: Application
    Filed: May 18, 2015
    Publication date: August 3, 2017
    Applicant: The Regents of the University of California
    Inventors: Daniel H. Arlow, William J. Holtz, Lane J. Weaver, Jay D. Keasling
  • Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound
    Patent number: 9637766
    Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: May 2, 2017
    Assignee: The Regents of the University of California
    Inventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling
  • Methods for increasing production of 3-methyl-2-butenol using fusion proteins
    Patent number: 9631210
    Abstract: The present invention relates to methods and compositions for increasing production of 3-methyl-2-butenol in a de novo synthetic pathway in a genetically modified host cell using isopentenyl disphosphate (IPP) as a substrate.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: April 25, 2017
    Assignee: The Regents of the University of California
    Inventors: Howard Chou, Jay D. Keasling
  • Production of fatty acids and derivatives thereof
    Patent number: 9598706
    Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: March 21, 2017
    Assignee: REG LIFE SCIENCES, LLC
    Inventors: Jay D. Keasling, Zhihao Hu, Chris Somerville, George Church, David Berry, Lisa C. Friedman, Andreas Schirmer, Shane Brubaker, Stephen B. Del Cardayre
  • HYBRID POLYKETIDE SYNTHASES
    Publication number: 20170015990
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.
    Type: Application
    Filed: May 2, 2016
    Publication date: January 19, 2017
    Inventors: Jeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
  • Host cells and methods for producing diacid compounds
    Publication number: 20170016034
    Abstract: The present invention provides for a genetically modified host cell and related methods and materials for the biocatalytic production of an ?,?-dicarboxylic acids (DCAs) and/or mono-methyl ester derivatives of dicarboxylic acids (DCAMMEs).
    Type: Application
    Filed: March 10, 2016
    Publication date: January 19, 2017
    Applicant: The Regents of the University of California
    Inventors: Robert W. Haushalter, Jay D. Keasling
  • Synthetic Polypeptide Having A Xylose Import Activity
    Publication number: 20170015714
    Abstract: This disclosure provides methods and compositions related to microbial gene expression. In one aspect, a synthetic polypeptide having a xylose import activity.
    Type: Application
    Filed: July 14, 2016
    Publication date: January 19, 2017
    Applicant: The Regents of the University of California
    Inventors: Aindrila Mukhopadhyay, Amanda Reider Apel, Mario Ouellet, Jay D. Keasling
  • Metabolic engineering of the shikimate pathway
    Patent number: 9540652
    Abstract: The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: January 10, 2017
    Assignee: The Regents of the University of California
    Inventors: Darmawi Juminaga, Jay D. Keasling
  • Host Cell Modified to Produce 2-Pyrrolidone
    Publication number: 20160304922
    Abstract: The present invention provides for a genetically modified host cell comprising a 2-pyrrolidone synthase, or an enzymatically active fragment thereof, heterologous to the host cell.
    Type: Application
    Filed: April 14, 2016
    Publication date: October 20, 2016
    Applicant: The Regents of the University of California
    Inventors: Jingwei Zhang, Jay D. Keasling
  • Host Cells and Methods for Producing 1-Deoxyxylulose 5-phosphate (DXP) and/or a DXP Derived Compound
    Publication number: 20160298153
    Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.
    Type: Application
    Filed: June 17, 2016
    Publication date: October 13, 2016
    Applicant: The Regents of the University of California
    Inventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling
  • Modified host cells with efflux pumps
    Patent number: 9428726
    Abstract: The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: August 30, 2016
    Assignee: The Regents of the University of California
    Inventors: Mary J. Dunlop, Jay D. Keasling, Aindrila Mukhopadhyay
  • Yeast Cell Modified to Overproduce Fatty Acid and Fatty Acid-Derived Compounds
    Publication number: 20160215308
    Abstract: The present invention provides for a genetically modified yeast host cell capable of producing one or more fatty acids, or fatty acid-derived compounds, or a mixture thereof, comprising: (a) increased expression of acetyl-CoA carboxylase (such as ACC1), (b) increased expression of one or more fatty acid synthases (such as FAS1 and FAS2), and (c) optionally reduced expression of one or more enzymes involved in or in the ?-oxidation pathway (such as peroxisomal transporters PXA1 and PXA2, and ?-oxidation enzymes POX1, POX2, and POX3).
    Type: Application
    Filed: January 25, 2016
    Publication date: July 28, 2016
    Applicant: The Regents of the University of California
    Inventors: Weerawat Runguphan, Jay D. Keasling
  • Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound
    Patent number: 9382553
    Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phosphate and/or ribulose 5-phosphate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: July 5, 2016
    Assignee: The Regents of the University of California
    Inventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling
  • Fusion proteins useful for producing pinene
    Patent number: 9376691
    Abstract: The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: June 28, 2016
    Assignee: The Regents of the University of California
    Inventors: Pamela P. Peralta-Yahya, Jay D. Keasling
  • Hybrid polyketide synthases
    Patent number: 9334514
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.
    Type: Grant
    Filed: October 31, 2011
    Date of Patent: May 10, 2016
    Assignee: The Regents of the University of California
    Inventors: Jeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
  • PRODUCING ALPHA-OLEFINS USING POLYKETIDE SYNTHASES
    Publication number: 20160068827
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an ?-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the ?-olefin.
    Type: Application
    Filed: September 14, 2015
    Publication date: March 10, 2016
    Inventors: Jeffrey L. Fortman, Leonard Katz, Eric J. Steen, Jay D. Keasling
  • Growth Arrested Cells Useful for Producing Compounds
    Publication number: 20150353939
    Abstract: The present invention provides for a genetically modified host cell in a growth-arrested state producing an antibiotic.
    Type: Application
    Filed: May 11, 2015
    Publication date: December 10, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Gregory E. Bokinsky, Jay D. Keasling
  • Synthetic Transcription Factor and Uses Thereof
    Publication number: 20150353614
    Abstract: The present invention provides for a synthetic transcription factor (TF) comprising a first peptide capable of binding a target ligand, a second peptide capable of binding a target DNA, and a peptide linker linking the first and second peptides. The present invention also provide for a system for modulating the mutagenesis frequency of a host cell. The host cell has a mutator rate (R) which is inversely proportional to a phenotypic trait (P).
    Type: Application
    Filed: June 10, 2015
    Publication date: December 10, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Howard H. Chou, Jay D. Keasling, Sergey Zotchev