Abstract: A cell separation process for the isolation of pathogenic cells in a small concentration in a biological fluid specimen, including treating the biological specimen to modify in a differential manner selected rare cells and other cells and causing differential migration of cells that reacted during treatment versus cells that did not react during treatment.

Abstract: A cell separation process for the isolation of pathogenic cells in a small concentration in a biological fluid specimen, including treating the biological specimen to modify in a differential manner selected rare cells and other cells and causing differential migration of cells that reacted during treatment versus cells that did not react during treatment.

Abstract: A process for magnetic immunoseparation of cells, in particular bacteria, fetal cells, stock cells of bone marrow and circulating cancerous cells, of the type consisting in affixing the target cells on paramagnetic balls and in causing a magnetic field to act on a sample containing the affixed cells, the free cells and the surplus paramagnetic balls, in order to isolate the paramagnetic balls, in that the sample is caused to circulate in a tube the section of which is much less than the length over which the magnetic field is applied.

Abstract: A method for counting cells and microorganisms in a fluid medium, particularly in food and biological fluids, by labelling with a colored or fluorescent label which selectively labels the microorganisms and cells to be detected, and counting the labelled microorganisms and cells, wherein said colored or fluorescent label is a stoichiometric label, particularly a DNA or RNA label, the microorganisms and cells are concentrated at the same time as or prior to counting by means of a fluid medium concentration step, and counting is achieved by measuring the overall brightness of the concentrated medium and comparing it with a calibration curve.

Abstract: The present invention relates to a procedure for counting faintly luminescent particles, and relates specifically to a procedure for counting cells and microorganisms marked with a colored or fluorescent stain or marker that selectively marks the microorganisms and cells to be counted, characterized by the fact that the said procedure is implemented with the aid of a photon-accumulator camera and includes the acquisition of at least one low-resolution image I, the threshold processing of the said low-resolution image in order to obtain at least one digital image Ib, at least one dimensional filtration stage, and the counting of the number N of objects belonging to a given class of sizes in the digital image Ib.

Abstract: The present invention relates to a process of automatic analysis of elements in weak concentration on a support of objects of low occurrence, having a high-contrast surface state, consisting of illuminating the observation zone with a first light source (5) emitting in a first wavelength range, and a second source (4) emitting in the excitation spectrum of the objects (23, 32) of low occurrence, proceeding to focus image acquisition means and after focusing triggering the accumulation of photons emitted by the objects (23, 32) of low occurrence illuminated with a source (4) emitting in the excitation spectrum of said objects (23, 32), characterized in that one proceeds to modify the focusing of the image aquisition means until maximization of the contrast of a zone (30) in said first wavelength range, said zone (30) being dissociatable from the observation zone illuminated from the second light source.

Abstract: A stage (1) for fixing at least one porous or non-porous medium (27) that carries one or more samples to be observed and to be analyzed by means of a microscope. The stage includes a hole (10) for receiving a plate (20; 28) for supporting the medium (27) and thus for observing the samples carried thereby. The stage is designed for pressing the medium (27) against the support plate (20; 28) by suction. The invention is applicable to the biological industry for rapid and indexed analysis under a microscope of filters and other media carrying multiple samples.

Abstract: A filter device for a plurality of liquid or gaseous fluid samples containing particles to be filtered, the device comprising a stand, at least one filter, upstream and downstream clamping blocks cooperating wit the filter and designed to be fixed together with an interposed perforated sealing gasket and are configured to be inserted through an opening in the stand, forced filtering mechanism and filtrate evacuation mechanism. The upstream clamping block is a removable independent tank block for enabling pretreating of samples introduced in wells thereof.

Abstract: A filter apparatus for filtering a plurality of liquid or gas fluid samples containing particles to be filtered, such as bacteria or cells. The apparatus includes a filter; a device for simultaneously and independently filtering fluid samples on the filter; and apparatus for force filtering the samples. The device for simultaneously and independently filtering fluid samples includes at least one gasket including a plurality of through perforations held clamped in a sealed manner against one face of the filter by appropriate clamps. The clamps have perforations located for being brought into alignment with the perforations through the gasket and for cooperating therewith to put an upstream face of the gasket into communication, via the filter, with a plurality of independent inlets for samples of fluid to be filtered, and to put a downstream face of the gasket into communication with a plurality of likewise independent outlets for the fluid samples.

Abstract: The present invention provides a flexible and transparent ribbon having two continuous longitudinal shoulders disposed along the edges of a face of the ribbon opposite that which is intended to support a plurality of samples to be processed. These shoulders are intended for magnetic, optical, computer or other coding for providing very accurate location, on the order of 10 microns, of elementary sample portions. A space is provided between the shoulders for containing by capillarity a film of appropriate (reactive, rinsing, etc. . . ) liquid. This ribbon may also be provided with micro-wells and is intended to be contained in a cassette of the type used for video tape recording purposes, having jaws and connected to an image acquisition device and to a microprocessor for control purposes and data processing.

Abstract: An automatic device for analyzing and cloning cellular cultures and bacteriological analysis, comprising an enclosure divided into two compartments, the first compartment of which contains: a circular transparent plate comprising several microwells disposed in one or more circular concentric rows, at least one special microscopic observation device, more especially of the phase contrast type, a device for the aspiration/injection of cellular medium or cells guaranteeing non contamination, a motor unit for providing the rotational as well as vertical and horizontal movements of said plate, with very accurate positioning in the three directions of movement, whereas the second compartment surrounds the first one and contains the regulation devices, and a cooling block containing the nutritive medium supply reservoirs. The automatic device is controlled by a microprocessor. The device of the present invention may be applied to the analysis and cloning of cellular cultures as well as for bacteriological analysis.

Abstract: The present invention relates to a modular apparatus for cell culture, coising in the same sealed enclosure (2) with isotherm walls (3):at least one device (1, R, E) for storing culture containers,at least one temperature regulation device (7),a closed circuit filtered laminar flow sterilization device (11, 12, 13, 14),a device for supplying and regulating gas, such as air, CO.sub.2, vapor and/or similar, and in that the enclosure possibly contains at least one additional device for carrying out any one of the conventional cell culture operations, or any other specific operation, and defining a modular work post chosen depending on the needs.The apparatus may also comprise an automatic and programmable handling arm (B) and a microprocessor for controlling and processing the data.