Patents by Inventor Jean-Luc Maloisel
Jean-Luc Maloisel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11807666Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.Type: GrantFiled: November 29, 2022Date of Patent: November 7, 2023Assignee: Cytiva BioProcess R&D ABInventors: Martin Hall, Karolina Busson, Jean-Luc Maloisel, Helena Skoglar
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Patent number: 11718643Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five- or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.Type: GrantFiled: November 11, 2013Date of Patent: August 8, 2023Assignee: Cytiva BioProcess R&D ABInventors: Jean-Luc Maloisel, Gustav Rodrigo, Bjorn Noren, Virendra Kumbhar
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Publication number: 20230191368Abstract: The invention discloses a separation matrix comprising a plurality of multimodal ligands covalently coupled to a support, wherein said support is a membrane comprising nonwoven polymer fibers and wherein said ligands are capable of interacting with a target biomacromolecule. Further, the invention discloses separation methods using the separation matrix.Type: ApplicationFiled: April 16, 2021Publication date: June 22, 2023Inventors: Jean-Luc Maloisel, Jimmy Hedin Dahlström, Marc Hummersone, Ola Lind, Sara Häggblad Sahlberg, Jon Lundqvist, Tania Ahmad
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Publication number: 20230091082Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.Type: ApplicationFiled: November 29, 2022Publication date: March 23, 2023Inventors: Martin Hall, Karolina Busson, Jean-Luc Maloisel, Helena Skoglar
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Patent number: 11542300Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of plasmaproteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.Type: GrantFiled: April 4, 2016Date of Patent: January 3, 2023Assignee: Cytiva BioProcess R&D ABInventors: Martin Hall, Karolina Busson, Jean-Luc Maloisel, Helena Skoglar
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Publication number: 20220362744Abstract: A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 ?m and the said porous particles have a gel phase distribution coefficient, expressed as KD for dextran of molecular weight 110 kDa, of 0.5-0.9.Type: ApplicationFiled: June 29, 2022Publication date: November 17, 2022Inventors: Ronnie Palmgren, Jean-Luc Maloisel, Gustav Rodrigo, Tomas Bjorkman
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Patent number: 11439926Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: GrantFiled: December 3, 2019Date of Patent: September 13, 2022Assignee: Cytiva BioProcess R&D ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Publication number: 20220266170Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: ApplicationFiled: May 10, 2022Publication date: August 25, 2022Inventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Patent number: 11285460Abstract: The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.Type: GrantFiled: February 18, 2015Date of Patent: March 29, 2022Assignee: CYTIVA BIOPROCESS R&D ABInventors: Jean-Luc Maloisel, Karolina Busson, Karol Lacki, Bjorn Noren, Helena Skoglar
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Patent number: 11053283Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.Type: GrantFiled: April 17, 2019Date of Patent: July 6, 2021Assignee: Cytiva BioProcess R&D ABInventors: Jean-Luc Maloisel, Ola Lind, Bjorn Noren, Ronnie Palmgren
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Patent number: 10864512Abstract: The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles (1) having a core region (2) and a shell region (3), wherein: a) said shell region is accessible to a target biomacromolecule; b) said core region is less accessible to the target biomacromolecule than the shell region; and c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.Type: GrantFiled: March 30, 2017Date of Patent: December 15, 2020Assignee: Cytiva BioProcess R&D ABInventors: Jean-Luc Maloisel, Tobias Söderman, Ola Lind, Ida Eklind
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Patent number: 10815269Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five-or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.Type: GrantFiled: August 29, 2018Date of Patent: October 27, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Jean-Luc Maloisel, Gustav Rodrigo, Bjorn Noren, Virendra Kumbhar
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Patent number: 10751645Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.Type: GrantFiled: January 29, 2018Date of Patent: August 25, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Carina Engstrand, Annika Forss, Gunnar Glad, Bo-Lennart Johansson, Hans J Johansson, Jean-Luc Maloisel
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Patent number: 10662261Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.Type: GrantFiled: March 27, 2018Date of Patent: May 26, 2020Assignee: GE Healthcare BioProcess R&D ABInventors: Mattias Algotsson, Lars C. Andersson, Eggert Brekkan, Lee Hedemyr, Jean-Luc Maloisel, Ronnie Palmgren
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Publication number: 20200101398Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: ApplicationFiled: December 3, 2019Publication date: April 2, 2020Applicant: GE Healthcare BioProcess R&D ABInventors: JAN BERGSTROM, GUNNAR GLAD, BO-LENNART JOHANSSON, JEAN-LUC MALOISEL, NILS NORRMAN, TOBIAS E. SODERMAN
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Publication number: 20190381480Abstract: A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 ?m and the said porous particles have a gel phase distribution coefficient, expressed as KD for dextran of molecular weight 110 kDa, of 0.5-0.9.Type: ApplicationFiled: February 15, 2018Publication date: December 19, 2019Inventors: Ronnie Palmgren, Jean-Luc Maloisel, Gustav Rodrigo, Tomas Bjorkman
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Patent number: 10493380Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.Type: GrantFiled: June 15, 2016Date of Patent: December 3, 2019Assignee: GE Healthcare BioProcess R&D ABInventors: Jan Bergstrom, Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman, Tobias E. Soderman
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Patent number: 10465153Abstract: The invention discloses a separation matrix with a porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which comprise at least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.Type: GrantFiled: November 26, 2014Date of Patent: November 5, 2019Assignee: GE HEALTHCARE BIOPROCESS R&D ABInventors: Jean-Luc Maloisel, Tobias Soderman, Ola Lind
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Publication number: 20190241608Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.Type: ApplicationFiled: April 17, 2019Publication date: August 8, 2019Applicant: GE Healthcare BioProcess R&D ABInventors: Jean-Luc Maloisel, Ola Lind, Bjorn Noren, Ronnie Palmgren
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Patent number: 10266563Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.Type: GrantFiled: June 4, 2015Date of Patent: April 23, 2019Assignee: GE Healthcare Bio-Process R&D ABInventors: Jean-Luc Maloisel, Ola Lind, Bjorn Noren, Ronnie Palmgren