Abstract: A method to image an in vivo object in an eye includes illuminating an object in the eye by a light source; configuring one or more detectors to receive light from a conjugate plane behind a confocal plane of the object, the conjugate plane acting as a light screen; receiving at the one or more detectors a backscattered light from the light source which has been refracted at least in part by the object before being backscattered from the light screen to provide a detector data; and processing the detector data over a time period by a computer to generate information about the object.

Abstract: A method for imaging vertebrate translucent retinal structures includes: imaging a translucent retinal structure at a first imaging plane in the retina with a light source focused at such first imaging plane, and detecting reflected light with a non-confocal off-axis detector, wherein the detector is axially displaced from a plane conjugate to the first imaging plane to a plane conjugate to a reflective layer deeper in the retina along a path of illumination from the light source.

Abstract: An image processing method for determining and reporting a velocity of a plurality of objects in a space-time image includes: providing an imager to acquire a plurality of images of a line; receiving a plurality of images; generating a space-time image from the plurality of images of the line; applying a mask to a subset image of the space-time image to provide a masked subset area; generating a rotational look up table of intensity values of pixels of columns and rows of the masked subset area; generating a radon transform for each subset image; computing a velocity of a plurality of objects in the subset image based on the radon transform; repeating the step of applying a mask to the step of computing a velocity; and displaying the velocity of a plurality of objects overlaying the space-time image substantially in real-time as the plurality of images are acquired.

Abstract: An ophthalmic imaging system for imaging an axial and lateral point-spread of light distribution pattern of light reflected from a surface of an animal or human eye includes an ophthalmic imaging apparatus adapted to generate an illumination light of a surface of the eye. A detection arm includes a digital micromirror device (DMD) having an array of mirror facets. A first detector is disposed to receive a first portion of the axial and lateral point-spread of light distribution of light, and one or a plurality of additional detectors are disposed to receive a light from one or a plurality of different portions of the axial and lateral point-spread of light distribution pattern of light. A detection arm, a method for imaging axial and lateral point-spread of light distribution components, and a method for auto-centering a distribution pattern in an imaging plane of a DMD device are also described.

Abstract: A method to image an in vivo object in an eye includes illuminating an object in the eye by a light source; configuring one or more detectors to receive light from a conjugate plane behind a confocal plane of the object, the conjugate plane acting as a light screen; receiving at the one or more detectors a backscattered light from the light source which has been refracted at least in part by the object before being backscattered from the light screen to provide a detector data; and processing the detector data over a time period by a computer to generate information about the object.

Abstract: A method for imaging vertebrate translucent retinal structures includes imaging a translucent retinal structure at a first imaging plane in the retina with a light source focused at such first imaging plane, and detecting reflected light with a non-confocal off-axis detector, wherein the detector is axially displaced from a plane conjugate to the first imaging plane to a plane conjugate to a reflective layer deeper in the retina along a path of illumination from the light source. The method may employ scanning ophthalmic instruments, and enables an enhancement in contrast and collected intensity by axially displacing the image detector away from the object of interest to positions closer to conjugate to layers of strong retinal reflection, like the photoreceptors or the interface between the choroid and the sclera, that act as a diffusive screen.

Abstract: An image processing method for determining and reporting a velocity of a plurality of objects in a space-time image includes: providing an imager to acquire a plurality of images of a line; receiving a plurality of images; generating a space-time image from the plurality of images of the line; applying a mask to a subset image of the space-time image to provide a masked subset area; generating a rotational look up table of intensity values of pixels of columns and rows of the masked subset area; generating a radon transform for each subset image; computing a velocity of a plurality of objects in the subset image based on the radon transform; repeating the step of applying a mask to the step of computing a velocity; and displaying the velocity of a plurality of objects overlaying the space-time image substantially in real-time as the plurality of images are acquired.

Abstract: An ophthalmic imaging system for imaging an axial and lateral point-spread of light distribution pattern of light reflected from a surface of an animal or human eye includes an ophthalmic imaging apparatus adapted to generate an illumination light of a surface of the eye. A detection arm includes a digital micromirror device (DMD) having an array of mirror facets. A first detector is disposed to receive a first portion of the axial and lateral point-spread of light distribution of light, and one or a plurality of additional detectors are disposed to receive a light from one or a plurality of different portions of the axial and lateral point-spread of light distribution pattern of light. A detection arm, a method for imaging axial and lateral point-spread of light distribution components, and a method for auto-centering a distribution pattern in an imaging plane of a DMD device are also described.

Abstract: A method for creating a virtual 2D image of an object moving in a blood vessel includes the steps of: providing an imaging apparatus capable of acquiring 1D images. The imaging apparatus is communicatively coupled to a computer. Multiple 1D images of a substantially fixed footprint on a surface of an organ are acquired over a period of time. A virtual 2D image showing the object moving in the blood vessel is formed by combining by computer the multiple 1D images. The virtual 2D image can be displayed and/or saved to a non-volatile memory. A system to perform the method is also described. Another method for creating a virtual 1D image of an object moving in a blood vessel is also described.

Abstract: A method for creating a virtual 2D image of an object moving in a blood vessel includes the steps of: providing an imaging apparatus capable of acquiring 1D images. The imaging apparatus is communicatively coupled to a computer. Multiple 1D images of a substantially fixed footprint on a surface of an organ are acquired over a period of time. A virtual 2D image showing the object moving in the blood vessel is formed by combining by computer the multiple 1D images. The virtual 2D image can be displayed and/or saved to a non-volatile memory. A system to perform the method is also described. Another method for creating a virtual 1D image of an object moving in a blood vessel is also described.