Abstract: A polynucleotide having promoter activity of a malate dehydrogenase gene (mdh gene), a transcription expression cassette, recombinant expression vector and recombinant host cell containing the polynucleotide having the promoter activity, a method for constructing a promoter mutant, a method for regulating the transcription of a target gene, a method for preparing a protein, and method for producing a target compound. The polynucleotide having the promoter activity is a mutant of an mdh gene promoter, and compared with a promoter of a wild-type mdh gene, the promoter activity of the mutant is significantly improved. After operably linking the mutant to a target gene, the expression efficiency of the target gene can be significantly improved, thereby effectively improving the yield and transformation rate of a target compound.

Abstract: A protein having an L-proline efflux function and the use thereof are provided. A method for producing L-proline or hydroxyproline by means of using a protein ThrE is used for producing L-proline by means of enhancing the activity of a polypeptide, having an L-proline efflux function, in an L-proline-producing strain. Alternatively, the method is used for producing hydroxyproline by means of weakening the activity of a polypeptide, having an L-proline efflux function, in L-proline-producing host cells and enhancing the activity of a proline hydroxylase.

Abstract: Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

Abstract: Provided are a polypeptide with aspartate kinase activity and the use thereof in the production of an amino acid. Specifically, provided are a novel polypeptide with aspartate kinase activity, a recombinant polypeptide, a polynucleotide, a nucleic acid construct, a recombinant expression vector, a recombinant host cell, and a method for producing an amino acid. The polypeptide with aspartate kinase activity is a mutant mutated at one or more positions corresponding to positions 293, 294 and 307 of the amino acid sequence as shown in SEQ ID NO: 1. Compared with a polypeptide having the sequence as shown in SEQ ID NO: 1, the mutant polypeptide removes the feedback inhibition of lysine on aspartate kinase, has high aspartate kinase activity, and can be used for the stable and efficient production of lysine and derivatives thereof.

Abstract: Provided are a mutant of a Corynebacterium glutamicum glutamate dehydrogenase gene promoter and applications thereof. The mutant has improved promoter activity compared to a wild-type promoter. Hence, it can be used to enhance the expression of a target gene, for example, operably ligating the mutant with a glutamate dehydrogenase gene, and the expression intensity of the glutamate dehydrogenase can be enhanced, thereby improving the amino acid production efficiency of a recombinant strain.

Abstract: Disclosed are a mutant of a pyruvate carboxylase gene promoter of Corynebacterium glutamicum and applications thereof. The mutant has improved promoter activity compared with a wild-type promoter, and can be used for enhancing expression of a target gene, for example, operably ligating the mutant to a pyruvate carboxylase gene enhances the expression intensity of the pyruvate carboxylase, thereby improving the production efficiency of amino acids of the strain.

Abstract: Provided are Corynebacterium glutamicum that produces L-lysine, a method for constructing the L-lysine producing strain, and a method for producing L-lysine from the strain. The lysine yield and glucose conversion of the L-lysine producing strain are improved, thereby reducing the production cost.

Abstract: A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA.

Abstract: The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.

Abstract: A photoimageable composition for preparing a polymeric binder having of one or more of monomer unit copolymers, a photoactive compound, and one or more organometallic compounds is provided. The copolymers include one or more hydroxyl or carboxyl functional groups that react with the organometallic compound to form a crosslinked network upon curing. The photoimageable compositions may be particularly useful in forming a pixel-defining layer of an electronic device. such as an organic light emitting diode. In particular, photoimageable compositions in accordance with embodiments of the present invention are insoluble in the developer prior to exposure to radiation, soluble in the developer following radiation exposure, and have relatively high glass transition temperatures (Tg) making them useful in forming a pixel-defining layer.

Abstract: Provided in the present invention is an asparaginic acid kinase, and the 340th amino acid residue in the position of the amino acid sequence of the asparaginic acid kinase corresponding to the amino acid sequence shown in SEQ ID NO: 2 is a non-aspartic acid. The asparaginic acid of the present invention can efficiently relieve the feedback inhibition of L-lysine, and can be effectively used for the production of L-lysine. Also provided in the present invention are host cells comprising genes coding the asparaginic acid kinase and a method for producing L-lysine using the host cells or the asparaginic acid kinase. The asparaginic acid kinase of the present invention or the host cells comprising the asparaginic acid kinase of the present invention is also used to produce L-threonine, L-methionine, L-isoleucine or L-valine. Also provided in the present invention is a method of producing L-aspartyl-4-yl phosphoric acid using the asparaginic acid kinase or the host cells.

Abstract: The present invention provides a polypeptide complex on the basis of Titin-Telethonin beta-pleated sheet structure as a polypeptide or protein drug carrier, a method of using the polypeptide complex, and a fusion protein complex thereof. The polypeptide complex is capable of maintaining the activity of polypeptide or protein drugs and prolonging the half-life period simultaneously.

Abstract: An interposer for coupling an optical conduit to an optical component, said interposer comprising: (a) an optical component; (b) a first lens component having a first lens; (c) a second lens component having a second lens, said first and second lenses being configured to define an expanded-beam coupling therebetween; (d) at least one reflective surface optically coupled with said second lens; (e) a first optical path at least partially defined between said optical component and said first lens to accommodate a diverging light beam from said optical component to said first lens; (f) a second optical path at least partially defined between said second lens and said at least one reflective surface to accommodate a converging light beam from said second lens and said at least one reflective surface; and (g) a separable interface along said second optical path or at said expanded-beam coupling.

Abstract: An interposer for coupling an optical conduit to an optical component, said interposer comprising: (a) an optical component; (b) a first lens component having a first lens; (c) a second lens component having a second lens, said first and second lenses being configured to define an expanded-beam coupling therebetween; (d) at least one reflective surface optically coupled with said second lens; (e) a first optical path at least partially defined between said optical component and said first lens to accommodate a diverging light beam from said optical component to said first lens; (f) a second optical path at least partially defined between said second lens and said at least one reflective surface to accommodate a converging light beam from said second lens and said at least one reflective surface; and (g) a separable interface along said second optical path or at said expanded-beam coupling.

Abstract: Expanded beam (EB) connector includes a fiber holder having an alignment channel that is configured to receive an optical fiber. The alignment channel has a channel opening and extends from the channel opening to a channel end face. The EB connector also includes an optical substrate having a three-dimensional (3D) waveguide that includes a waveguide core and a cladding. The optical substrate includes the channel end face. The waveguide core extends lengthwise between first and second coupling faces of the waveguide core. The first coupling face is at least a portion of the channel end face. The first coupling face is configured to optically couple to the optical fiber disposed within the alignment channel. The second coupling face defines an exterior of the optical substrate. The waveguide core is shaped to change a mode field diameter and a numerical aperture of light propagating between the first and second coupling faces.

Abstract: An electrochromic test port provides an actively tunable system for building an optical test port for an optical waveguide with enhanced SNR properties over conventional approaches.

Abstract: Expanded beam (EB) connector includes a fiber holder having an alignment channel that is configured to receive an optical fiber. The alignment channel has a channel opening and extends from the channel opening to a channel end face. The EB connector also includes an optical substrate having a three-dimensional (3D) waveguide that includes a waveguide core and a cladding. The optical substrate includes the channel end face. The waveguide core extends lengthwise between first and second coupling faces of the waveguide core. The first coupling face is at least a portion of the channel end face. The first coupling face is configured to optically couple to the optical fiber disposed within the alignment channel. The second coupling face defines an exterior of the optical substrate. The waveguide core is shaped to change a mode field diameter and a numerical aperture of light propagating between the first and second coupling faces.

Abstract: Array connector includes a connector body having a mating side. The connector body includes a plurality of substrate layers that are stacked side-by-side and have respective mating edges that form the mating side. The substrate layers form a plurality of interfaces in which each interface is defined between adjacent substrate layers. The adjacent substrate layers of each interface are shaped to form a plurality of channels. The array connector also includes communication lines that are disposed within corresponding channels of the connector body such that the communication lines extend along the interfaces. The communication lines are at least one of wire conductors or optical fibers. The communication lines have respective mating terminals that are positioned proximate to the mating side and form a terminal array.

Abstract: Probe assembly includes a modular device configured to detect external signals or emit energy. The modular device has a device array that includes at least one of electrical contacts or optical fiber ends. The probe assembly also includes a cable assembly that is configured to communicatively couple the modular device to a computing system and transmit data signals therethrough. The cable assembly includes an array connector having a connector body that includes a mating side and channels extending through the mating side and the connector body. The cable assembly includes a plurality of communication lines that are disposed within corresponding channels of the connector body. The communication lines have respective end faces that are positioned proximate to the mating side to form a terminal array. The terminal array is aligned with and coupled to the device array of the modular device.

Abstract: The invention provides a composition comprising at least the following A and B: A) a polymer comprising, in polymerized from, at least one “monomer that comprises at least one hydroxyl group;” and B) an organometal compound comprising at least one metal selected from Ti, Zr, Hf, Co, Mn, Zn, or combinations thereof, and wherein the organometal compound is present in an amount greater than 5 weight percent, based on the sum weight of A and B.