Abstract: Provided in the present disclosure is a preparation method of paeoniflorin-6?-O-benzene sulfonate. The method uses crude paeoniflorin instead of paeoniflorin as a raw material, which more complies with actual requirements of an industrial production process of paeoniflorin-6?-O-benzene sulfonate, and uses an organometallic catalyst, an acid-binding agent and chemically active benzenesulfonyl chloride to react with paeoniflorin. Compared with the prior art, the method significantly increases the yield of crude paeoniflorin-6?-O-benzene sulfonate which is separated and purified by means of column chromatography and then refined to obtain paeoniflorin-6?-O-benzenesulfonate with a content of at least 98%, thereby realizing industrial production of the paeoniflorin-6?-O-benzene sulfonate.

Abstract: GFRAL extracellular domains comprising domains D2 and D3 are disclosed. The disclosure further relates to methods and compositions for screening and evaluating the activity of a GFRAL ligand, such as a GDF15 peptide, using the GFRAL extracellular domains provided herein. Also disclosed are methods and compositions for treating obesity, reducing appetite, and/or reducing body weight using the GFRAL extracellular domains provided herein.