Patents by Inventor Johannes Gustaaf Ernst VAN LEEUWEN

Johannes Gustaaf Ernst VAN LEEUWEN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210348136
    Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Application
    Filed: July 21, 2021
    Publication date: November 11, 2021
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Priscilla ZWARTJENS, Viktor Marius BOER
  • Patent number: 11104886
    Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Grant
    Filed: December 4, 2017
    Date of Patent: August 31, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Gustaaf Ernst Van Leeuwen, Priscilla Zwartjens, Viktor Marius Boer
  • Publication number: 20210238235
    Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.
    Type: Application
    Filed: April 29, 2019
    Publication date: August 5, 2021
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Viktor Marius BOER, Priscilla ZWARTJENS, Jos VAN VUGT, René Marcel DE JONG
  • Publication number: 20210147816
    Abstract: The present disclosure relates a polypeptide having UGT activity, which polypeptide comprises an amino acid sequence which, when aligned with a polypeptide having UGT activity comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 35, 189, 280, 284, 285, 334 or 373, said positions being defined with reference to SEQ ID NO: 2 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having UGT activity. A polypeptide according to the disclosure may be used in a recombinant cell for the production of steviol or a steviol glycoside.
    Type: Application
    Filed: June 26, 2018
    Publication date: May 20, 2021
    Inventors: Viktor Marius BOER, Johannes Gustaaf Ernst VAN LEEUWEN
  • Publication number: 20210147815
    Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.
    Type: Application
    Filed: January 27, 2021
    Publication date: May 20, 2021
    Inventors: Viktor Marius BOER, Johannes Gustaaf Ernst VAN LEEUWEN, Priscilla ZWARTJENS, Catharina Petronella Antonia KOLEN
  • Publication number: 20210123030
    Abstract: The invention relates to a polypeptide having cellulolytic enhancing activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 60% sequence identity with the amino acid sequence of SEQ ID NO: 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44 and/or 47; (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% sequence identity to the nucleotide sequence of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which hybridises under at least high stringency conditions with the complementary strand of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; and (d) a fragment of the polypeptide of (a), (b), or (c), that has cellulolytic enhancing activity.
    Type: Application
    Filed: December 30, 2020
    Publication date: April 29, 2021
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Loes Elizabeth BEVERS
  • Publication number: 20210040491
    Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Application
    Filed: July 24, 2020
    Publication date: February 11, 2021
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Priscilla Zwartjens, Viktor Marius Boer
  • Patent number: 10913938
    Abstract: The invention relates to a polypeptide having cellulolytic enhancing activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 60% sequence identity with the amino acid sequence of SEQ ID NO: 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44 and/or 47; (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% sequence identity to the nucleotide sequence of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which hybridises under at least high stringency conditions with the complementary strand of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; and (d) a fragment of the polypeptide of (a), (b), or (c), that has cellulolytic enhancing activity.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: February 9, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Gustaaf Ernst Van Leeuwen, Loes Elizabeth Bevers
  • Publication number: 20200392475
    Abstract: The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.
    Type: Application
    Filed: August 31, 2020
    Publication date: December 17, 2020
    Inventor: Johannes Gustaaf Ernst VAN LEEUWEN
  • Patent number: 10808235
    Abstract: The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: October 20, 2020
    Assignee: DSM IP Assets B.V.
    Inventor: Johannes Gustaaf Ernst Van Leeuwen
  • Patent number: 10760085
    Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: September 1, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Gustaaf Ernst Van Leeuwen, Priscilla Zwartjens, Viktor Marius Boer
  • Patent number: 10604743
    Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.
    Type: Grant
    Filed: March 16, 2016
    Date of Patent: March 31, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Viktor Marius Boer, Johannes Gustaaf Ernst Van Leeuwen, Priscilla Zwartjens, Catharina Petronella Antonia Maria Kolen
  • Publication number: 20200032225
    Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Application
    Filed: December 4, 2017
    Publication date: January 30, 2020
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Priscilla ZWARTJENS, Viktor Marius BOER
  • Publication number: 20190345461
    Abstract: The invention relates to a polypeptide having cellulolytic enhancing activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 60% sequence identity with the amino acid sequence of SEQ ID NO: 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44 and/or 47; (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% sequence identity to the nucleotide sequence of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which hybridises under at least high stringency conditions with the complementary strand of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; and (d) a fragment of the polypeptide of (a), (b), or (c), that has cellulolytic enhancing activity.
    Type: Application
    Filed: July 27, 2017
    Publication date: November 14, 2019
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Loes Elizabeth BEVERS
  • Publication number: 20190284539
    Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Application
    Filed: October 26, 2017
    Publication date: September 19, 2019
    Applicant: DSM IP ASSETS B.V.
    Inventors: Viktor Marius BOER, Priscilla ZWARTJENS, Johannes Gustaaf Ernst VAN LEEUWEN
  • Patent number: 10316306
    Abstract: The present invention relates to an alpha amylase enzyme and to its use in the food, feed and non-food industry.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: June 11, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Hanna Elisabet Abbas, Jan Metske Van Der Laan, Johannes Gustaaf Ernst Van Leeuwen
  • Publication number: 20190078105
    Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Application
    Filed: October 5, 2016
    Publication date: March 14, 2019
    Inventors: Johannes Gustaaf Ernst VAN LEEUWEN, Priscilla ZWARTJENS, Viktor Marius BOER
  • Publication number: 20190002859
    Abstract: The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.
    Type: Application
    Filed: December 8, 2016
    Publication date: January 3, 2019
    Inventor: Johannes Gustaaf Ernst VAN LEEUWEN
  • Publication number: 20190002860
    Abstract: The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.
    Type: Application
    Filed: December 8, 2016
    Publication date: January 3, 2019
    Inventors: Hein Jacob WIJMA, Dirk Barend JANSSEN, Johannes Gustaaf Ernst VAN LEEUWEN
  • Publication number: 20180362945
    Abstract: The application relates to a polypeptide having beta-glucosidase activity, its method of production and its uses.
    Type: Application
    Filed: December 8, 2016
    Publication date: December 20, 2018
    Inventor: Johannes Gustaaf Ernst VAN LEEUWEN